FAM71A (NM_153606) Human Tagged ORF Clone

CAT#: RC206147

FAM71A (Myc-DDK-tagged)-Human family with sequence similarity 71, member A (FAM71A)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_153606" in other vectors (4)

Reconstitution Protocol

USD 553.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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Rabbit Polyclonal Anti-FAM71A Antibody
    • 100 ul

USD 539.00

Other products for "FAM71A"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FAM71A
Synonyms GARI-L4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206147 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGCTGATTTTCTGCTGCCGTATTATACGGCCCAGAGTGGCTCCAGCATGAGCATGTTCGACACCA
CCATGGGGAAACTGCAGCGACAACTGTACAAGGGGGAGTACGATATATTCAAGTATGCACCGATATTTGA
GAGCGACTTTATCCAGATCACCAAAAGGGGAGAAGTGATTGATGTGCACAACCGTGTCCGTATGGTGACC
ATGGGCATTGCACGTACCAGCCCCATCCTCCCACTCCCAGATGTCATGCTACTGGCACGACCGGCCACCG
GCTGCGAAGAGTATGCTGGACATGGCCAGGCCACCAAGAGAAAAAAACGCAAGGCAGCAAAGAACTTAGA
GCTCACCAGGCTTCTGCCCCTGAGGTTTGTACGGATCTCTGTTCAAGACCATGAGAAACAACAGCTGCGC
CTGAAGTTCGCCACTGGCAGATCTTGCTATCTGCAATTGTGTCCCGCTCTTGACACACGGGATGACCTCT
TTGCCTATTGGGAAAAACTAATTTACCTCTTGCGGCCACCCATGGAGAGTAACAGCAGTACCTGTGGCAT
TCCAGCTGAAGACATGATGTGGATGCCTGTGTTTCAGGAAGACAGGAGGAGCCTGGGAGCCGTGAACCTT
CAAGGAAAGGGGGATCAGGACCAGGTCAGCATCCAAAGCCTCCACATGGTCTCTGAGGTGTGTGGGGCCA
CCTCTGCTGCTTATGCTGGAGGGGAGGGACTCCAAAATGACTTTAACAAACCCACTAATGTGCTCAATGC
ATCCATCCCCAAAACATCTACAGAACTTGCTGAGGAGCCAGCAACAGGGGGGATTAAAGAGGCAGCAGCA
GCAGGGGCAGCTGCAGGGGCAGCAACAGGCACCGTAGCAGGTGCCTTGAGTGTGGCAGCAGCCAATTCTG
CCCCTGGACAGGTGAGCGCAGCCATAGCTGGGGCGGCCACCATCGATGCAGGAGGAAACAAAGGCAACAT
GGCCCTTGCAGGCACTGCCAGCATGGCTCCAAACAGCACGAAGGTGGCTGTGGCAGGGGCTGCAGGCAAG
TCCTCAGAGCATGTTTCCAGCGCATCCATGAGCCTTTCCCGAGAGGGCAGTGTGAGCCTGGCCATTGCAG
GAGTAGTACTGACCAGCAGGACAGCTGCAGAAGCAGACATGGATGCAGCAGCGGGACCTCCCGTCTCCAC
CCGGCAGAGCAAGAGCAGCCTGAGTGGACAGCATGGAAGGGAGCGAACCCAGGCCAGCGCTGAAGGCTGC
AAGGAGGGGAGGGAAAGAAGGGAAAAGGACAGGGCTCTCGGAAGGAGTTCCCATCGCCGCAGGACAGGTG
AAAGCCGCCACAAAACAAGGGGAGACAAGATTGCCCAAAAGTCCTCCAGCAGGTCCTCATTCAGCCACAG
AGCCAATAGAGATGACAAAAAGGAGAAAGGCTGTGGCAACCCGGGGAGCAGCAGGCACAGGGACTCGCAT
AAAGGTGTCAGCCACACGCCCATCTCAAAGGAGTCCAGGACCTCTCACAAATCTGGGAGGAGCTTATGGA
CCACCAGTTCCGGTTCCAGCAAGGGACTTGGCAGGGTCAGCTCTTTCCTGAGGAACGTCAGAGCCAACCT
TACTACAAAAGTAGTGGGCACACCACATGGCAGAGATGTGGACGTCATGGCTAAGATGGCGGAGAGGAGC
ACCAACGTGGCCATCGCCGAGACAGCAGAGGGTGGCCAGGGGCTGGAGATGGTTGGTTCTATGACACCGG
ACATCATGGAGACAGTGACCTTTGAAGCCCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206147 protein sequence
Red=Cloning site Green=Tags(s)

MNADFLLPYYTAQSGSSMSMFDTTMGKLQRQLYKGEYDIFKYAPIFESDFIQITKRGEVIDVHNRVRMVT
MGIARTSPILPLPDVMLLARPATGCEEYAGHGQATKRKKRKAAKNLELTRLLPLRFVRISVQDHEKQQLR
LKFATGRSCYLQLCPALDTRDDLFAYWEKLIYLLRPPMESNSSTCGIPAEDMMWMPVFQEDRRSLGAVNL
QGKGDQDQVSIQSLHMVSEVCGATSAAYAGGEGLQNDFNKPTNVLNASIPKTSTELAEEPATGGIKEAAA
AGAAAGAATGTVAGALSVAAANSAPGQVSAAIAGAATIDAGGNKGNMALAGTASMAPNSTKVAVAGAAGK
SSEHVSSASMSLSREGSVSLAIAGVVLTSRTAAEADMDAAAGPPVSTRQSKSSLSGQHGRERTQASAEGC
KEGRERREKDRALGRSSHRRRTGESRHKTRGDKIAQKSSSRSSFSHRANRDDKKEKGCGNPGSSRHRDSH
KGVSHTPISKESRTSHKSGRSLWTTSSGSSKGLGRVSSFLRNVRANLTTKVVGTPHGRDVDVMAKMAERS
TNVAIAETAEGGQGLEMVGSMTPDIMETVTFEAH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_153606
ORF Size 1782 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_153606.2
RefSeq Size 2332 bp
RefSeq ORF 1785 bp
Locus ID 149647
UniProt ID Q8IYT1
Cytogenetics 1q32.3
MW 63.3 kDa
Gene Summary This gene encodes a protein that interacts with the Rab2B small GTPase and may be important for integrity of the Golgi body. A knockdown of this gene induces fragmentation of the Golgi, similar to the effect seen with a knockdown of the Rab2B small GTPase. The encoded protein has an N-terminal Rab-binding domain specific for Rab2B. [provided by RefSeq, Feb 2017]

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