CATSPER1 (NM_053054) Human Tagged ORF Clone

CAT#: RC206133

CATSPER1 (Myc-DDK-tagged)-Human cation channel, sperm associated 1 (CATSPER1)

Product Images

Circular map for RC206133

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CATSPER1
• Synonyms: CATSPER; SPGF7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206133 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAAAACTCAGTGCCTGAAAAGGCTCAGAATGAGGCAGACACAAATAATGCAGATAGGTTCTTTC
  GCTCTCACTCATCACCCCCACACCACAGGCCAGGCCACAGCAGAGCTCTCCACCATTACGAGTTGCACCA
  TCACGGCGTGCCCCACCAACGTGGTGAATCTCACCACCCTCCGGAGTTCCAAGACTTCCACGACCAAGCC
  TTGTCCTCCCATGTCCACCAATCTCACCACCACAGCGAGGCACGGAATCACGTCAGAGCCCATGGCCCCA
  CAGGCTTTGGTCTGGCTCCCTCTCAAGGCGCCGTCCCCTCCCACCGTTCCTACGGTGAGGACTACCATGA
  TGAGCTCCAACGTGATGGCAGGAGGCATCATGATGGGTCCCAATACAGTGGGTTCCATCAGCAGAGTGAC
  TCCCATTACCATAGGGGGTCTCACCATGGCAGACCCCAATATCTCGGTGAGAATTTATCCCACTATTCCT
  CTGGCGTGCCCCACCACGGTGAGGCTTCCCACCATGGTGGGTCCTACCTCCCCCATGGACCCAATCCCTA
  CAGTGAGTCCTTCCACCACAGCGAGGCTTCCCACCTTAGCGGGCTCCAACACGATGAGTCCCAGCATCAC
  CAAGTCCCCCACCGTGGCTGGCCCCACCATCACCAAGTCCACCACCATGGCAGGTCCCGTCATCATGAAG
  CCCACCAGCATGGAAAGTCTCCTCATCACGGAGAGACCATTTCCCCTCATTCCTCTGTGGGGTCCTACCA
  GCGTGGGATATCTGACTATCACAGCGAGTACCACCAAGGTGATCACCACCCCAGTGAGTACCACCATGGC
  GACCATCCCCACCACACACAGCACCACTACCACCAGACCCACCGGCACCGAGACTACCATCAGCACCAAG
  ACCACCACGGCGCGTATCATTCCAGTTACCTCCATGGCGACTACGTCCAGAGCACTTCCCAACTCTCTAT
  CCCACACACATCCCGGAGCCTGATTCACGATGCCCCCGGCCCTGCTGCTTCTCGTACAGGAGTCTTCCCC
  TATCACGTAGCACACCCACGGGGCTCGGCTCACAGCATGACTCGGTCCTCCAGCACAATCCGCTCACGTG
  TCACCCAGATGTCCAAAAAAGTCCATACCCAGGATATCTCCACCAAACATTCAGAAGACTGGGGCAAAGA
  AGAAGGGCAATTTCAGAAACGCAAAACCGGCCGGCTCCAGCGGACCCGCAAGAAGGGACACTCTACCAAT
  CTCTTCCAGTGGCTGTGGGAAAAGCTAACCTTCCTCATTCAGGGCTTCCGGGAAATGATCCGGAACCTGA
  CCCAATCCTTGGCCTTTGAAACTTTCATCTTCTTCGTTGTCTGCCTCAACACCGTCATGCTGGTGGCCCA
  GACCTTCGCTGAAGTCGAGATCCGGGGCGAGTGGTACTTCATGGCCTTGGACTCCATATTCTTCTGCATC
  TACGTGGTGGAAGCCCTGCTCAAGATCATCGCCCTGGGCCTCTCGTACTTCTTTGACTTCTGGAACAATT
  TGGACTTCTTCATTATGGCCATGGCCGTGCTGGACTTCTTGCTGATGCAGACCCACTCCTTCGCCATCTA
  CCACCAAAGCCTCTTCCGGATCCTCAAGGTCTTCAAGAGCCTGCGGGCCCTGAGGGCAATCCGGGTCCTG
  CGGAGGCTCAGCTTCCTGACCAGCGTCCAGGAAGTGACAGGGACCCTGGGCCAGTCCTTGCCGTCCATCG
  CAGCCATCCTCATCCTCATGTTTACCTGCCTCTTCCTCTTCTCCGCGGTCCTCCGGGCACTGTTCCGCAA
  ATCTGACCCCAAGCGCTTCCAGAACATCTTCACCACCATCTTCACCCTCTTCACCTTGCTCACGCTGGAT
  GACTGGTCCCTCATCTACATGGACAGCCGTGCCCAGGGCGCCTGGTACATCATTCCCATCCTCATAATTT
  ACATCATCATCCAGTACTTCATCTTCCTCAACCTGGTGATTACTGTCCTGGTGGATAGCTTCCAGACGGC
  GCTGTTCAAAGGCCTTGAGAAAGCGAAGCAGGAGAGGGCCGCCCGGATCCAAGAGAAGCTGCTGGAAGAC
  TCACTGACGGAGCTCAGAGCTGCAGAGCCCAAAGAGGTGGCGAGTGAAGGCACCATGCTGAAGCGGCTCA
  TCGAGAAAAAGTTTGGGACCATGACTGAGAAGCAGCAGGAGCTCCTGTTCCATTACCTGCAGCTGGTGGC
  AAGCGTGGAGCAGGAGCAGCAGAAGTTCCGCTCCCAGGCAGCCGTCATCGATGAGATTGTGGACACCACA
  TTTGAGGCTGGAGAAGAGGACTTCAGGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206133 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQA
  LSSHVHQSHHHSEARNHVRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYSGFHQQSD
  SHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHH
  QVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHG
  DHPHHTQHHYHQTHRHRDYHQHQDHHGAYHSSYLHGDYVQSTSQLSIPHTSRSLIHDAPGPAASRTGVFP
  YHVAHPRGSAHSMTRSSSTIRSRVTQMSKKVHTQDISTKHSEDWGKEEGQFQKRKTGRLQRTRKKGHSTN
  LFQWLWEKLTFLIQGFREMIRNLTQSLAFETFIFFVVCLNTVMLVAQTFAEVEIRGEWYFMALDSIFFCI
  YVVEALLKIIALGLSYFFDFWNNLDFFIMAMAVLDFLLMQTHSFAIYHQSLFRILKVFKSLRALRAIRVL
  RRLSFLTSVQEVTGTLGQSLPSIAAILILMFTCLFLFSAVLRALFRKSDPKRFQNIFTTIFTLFTLLTLD
  DWSLIYMDSRAQGAWYIIPILIIYIIIQYFIFLNLVITVLVDSFQTALFKGLEKAKQERAARIQEKLLED
  SLTELRAAEPKEVASEGTMLKRLIEKKFGTMTEKQQELLFHYLQLVASVEQEQQKFRSQAAVIDEIVDTT
  FEAGEEDFRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053054
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053054.4
• RefSeq Size: 2634 bp
• RefSeq ORF: 2343 bp
• Locus ID: 117144
• UniProt ID: Q8NEC5
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 90.2 kDa
• Gene Summary: Calcium ions play a primary role in the regulation of sperm motility. This gene belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. The protein family features a single repeat with six membrane-spanning segments and a predicted calcium-selective pore region. [provided by RefSeq, Jul 2008]