TEX10 (NM_017746) Human Tagged ORF Clone

CAT#: RC205997

TEX10 (Myc-DDK-tagged)-Human testis expressed 10 (TEX10), transcript variant 1

Product Images

Circular map for RC205997

• 

  Western blot validation of overexpression lysate (Cat# LY413572) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205997 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TEX10
• Synonyms: bA208F1.2; Ipi1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205997 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAAAAAAGAAAACGCCAACATGATTTTCAAAAAGTAAAATTGAAAGTTGGTAAAAAGAAGCCCA
  AGTTACAAAATGCTACTCCTACAAACTTTAAAACAAAGACTATACATCTGCCTGAGCAACTCAAAGAGGA
  TGGAACACTTCCAACAAACAATAGAAAACTTAACATAAAGGATTTGCTGTCACAGATGCATCACTACAAT
  GCTGGGGTTAAACAAAGTGCTCTTCTTGGACTTAAAGACCTTTTGTCTCAATACCCATTTATAATTGATG
  CACACCTTTCAAACATATTAAGTGAAGTGACTGCTGTGTTTACAGATAAAGATGCTAATGTACGATTAGC
  AGCAGTTCAACTTCTTCAATTCCTGGCCCCCAAAATACGAGCTGAACAAATTTCTCCATTTTTTCCTTTG
  GTAAGTGCCCATCTCTCTAGTGCCATGACTCACATTACTGAAGGAATTCAGGAGGACTCTTTAAAAGTTT
  TGGACATTCTGCTGGAACAGTACCCAGCTCTAATTACTGGCCGTAGCAGCATATTGCTTAAGAATTTTGT
  AGAACTTATTTCTCATCAGCAGCTGTCCAAAGGACTGATAAATAGAGACAGATCCCAGTCCTGGATACTT
  TCTGTAAATCCTAATCGGAGACTCACTTCTCAGCAATGGAGGCTGAAAGTCTTAGTGAGACTCAGTAAAT
  TCCTTCAGGCCTTGGCAGATGGATCCAGTAGGTTGAGAGAAAGTGAAGGACTTCAGGAACAGAAAGAAAA
  TCCCCATGCCACTAGCAACTCCATTTTTATCAACTGGAAGGAACATGCCAACGACCAGCAACACATCCAG
  GTTTATGAAAATGGGGGTTCACAGCCAAATGTCAGTTCACAGTTCAGGCTACGGTATCTGGTTGGAGGAC
  TGAGTGGTGTGGATGAAGGCCTGTCATCTACTGAAAACCTGAAAGGATTTATTGAGATAATAATTCCATT
  GCTAATTGAATGCTGGGTTGAAGCTGTACCTCCACAACTAGCTACTCCTGTTGGGAATGGTATAGAACGA
  GAACCTCTACAGGTTATGCAGCAAGTTCTTAATATTATTTCCCTTCTGTGGAAACTCTCTAAACAACAGG
  ATGAAACCCATAAATTGGAGTCATGGCTTCGAAAGAACTACCTTATTGATTTTAAACACCATTTTATGAG
  TCGTTTTCCATATGTCTTAAAAGAAATAACCAAGCACAAAGGGAAAGAGCCAAATAAAAGCATCAAGCAT
  TGCACAGTTCTCTCCAATAACATAGATCATCTCTTACTGAATTTAACACTGTCTGATATCATGGTCTCCC
  TGGCAAATGCGTCAACTTTGCAGAAGGATTGCAGTTGGATAGAAATGATAAGGAAATTTGTAACAGAGAC
  CCTTGAAGATGGCTCTAGGCTAAATAGTAAGCAACTGAACAGATTGCTGGGAGTATCCTGGAGGTTAATG
  CAAATACAGCCAAACAGAGAGGACACAGAGACTCTTATTAAGGCAGTTTATACATTATATCAGCAGAGGG
  GCCTTATCCTTCCAGTTCGGACTTTGTTATTGAAGCTTTTCAGTAAAATCTATCAGACAGAAGAACTGAG
  ATCTTGTAGATTCAGATATCGTAGTAAAGTGTTATCCCGTTGGCTGGCTGGCTTACCATTGCAACTTGCT
  CATCTTGGCTCCCGAAATCCTGAGCTCTCTACACAGCTTATCGATATCATTCATACCGCTGCAGCACGAG
  CAAATAAAGAATTACTAAAAAGTTTACAAGCTACTGCCCTCCGAATTTATGATCCACAAGAAGGTGCTGT
  GGTGGTTCTCCCTGCAGACTCTCAGCAGCGTTTGGTTCAGCTTGTATATTTCCTACCCAGTCTGCCGGCT
  GATTTGCTTTCTCGGTTAAGTCGTTGCTGTATTATGGGAAGACTCAGTTCAAGTTTGGCTGCCATGCTTA
  TCGGGATACTGCACATGAGATCATCATTTTCTGGGTGGAAGTATTCAGCTAAAGACTGGTTGATGAGTGA
  TGTAGACTATTTCAGCTTCTTATTTTCCACACTTACAGGGTTTTCGAAAGAGGAGTTGACTTGGCTTCAG
  AGCCTTCGAGGAGTTCCTCATGTCATCCAGACACAGCTTTCCCCTGTGCTTCTCTACCTTACAGATTTGG
  ATCAATTTTTACACCACTGGGATGTAACAGAGGCAGTTTTTCACAGTTTATTGGTTATTCCTGCCCGAAG
  TCAGAACTTTGACATCTTGCAAAGTGCCATCAGTAAGCATTTGGTTGGGTTGACTGTAATTCCTGACAGC
  ACGGCTGGCTGTGTTTTTGGTGTTATCTGTAAGCTCCTGGATCATACTTGTGTAGTTAGTGAGACTCTAC
  TGCCATTTCTGGCTTCTTGTTGCTACAGTCTTCTTTATTTTCTGCTCACTATAGAGAAAGGGGAAGCAGA
  ACATCTAAGAAAGAGGGACAAGCTGTGGGGGGTCTGTGTCTCCATCCTGGCTCTCTTGCCTCGAGTCCTC
  AGGTTGATGCTGCAGAGCCTGCGGGTGAACAGAGTTGGGCCTGAGGAGCTGCCTGTTGTGGGCCAGCTGC
  TTCGACTGCTGCTTCAGCATGCACCCCTCAGGACTCATATGTTGACCAATGCGATCTTGGTGCAGCAGAT
  CATCAAGAATATCACGACATTGAAGAGTGGAAGTGTTCAGGAACAGTGGCTCACAGACTTACATTACTGC
  TTTAACGTGTATATCACTGGGCATCCCCAAGGGCCCAGTGCACTGGCTACAGTGTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205997 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTKKRKRQHDFQKVKLKVGKKKPKLQNATPTNFKTKTIHLPEQLKEDGTLPTNNRKLNIKDLLSQMHHYN
  AGVKQSALLGLKDLLSQYPFIIDAHLSNILSEVTAVFTDKDANVRLAAVQLLQFLAPKIRAEQISPFFPL
  VSAHLSSAMTHITEGIQEDSLKVLDILLEQYPALITGRSSILLKNFVELISHQQLSKGLINRDRSQSWIL
  SVNPNRRLTSQQWRLKVLVRLSKFLQALADGSSRLRESEGLQEQKENPHATSNSIFINWKEHANDQQHIQ
  VYENGGSQPNVSSQFRLRYLVGGLSGVDEGLSSTENLKGFIEIIIPLLIECWVEAVPPQLATPVGNGIER
  EPLQVMQQVLNIISLLWKLSKQQDETHKLESWLRKNYLIDFKHHFMSRFPYVLKEITKHKGKEPNKSIKH
  CTVLSNNIDHLLLNLTLSDIMVSLANASTLQKDCSWIEMIRKFVTETLEDGSRLNSKQLNRLLGVSWRLM
  QIQPNREDTETLIKAVYTLYQQRGLILPVRTLLLKLFSKIYQTEELRSCRFRYRSKVLSRWLAGLPLQLA
  HLGSRNPELSTQLIDIIHTAAARANKELLKSLQATALRIYDPQEGAVVVLPADSQQRLVQLVYFLPSLPA
  DLLSRLSRCCIMGRLSSSLAAMLIGILHMRSSFSGWKYSAKDWLMSDVDYFSFLFSTLTGFSKEELTWLQ
  SLRGVPHVIQTQLSPVLLYLTDLDQFLHHWDVTEAVFHSLLVIPARSQNFDILQSAISKHLVGLTVIPDS
  TAGCVFGVICKLLDHTCVVSETLLPFLASCCYSLLYFLLTIEKGEAEHLRKRDKLWGVCVSILALLPRVL
  RLMLQSLRVNRVGPEELPVVGQLLRLLLQHAPLRTHMLTNAILVQQIIKNITTLKSGSVQEQWLTDLHYC
  FNVYITGHPQGPSALATVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017746
• ORF Size: 2787 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017746.4
• RefSeq Size: 3129 bp
• RefSeq ORF: 2790 bp
• Locus ID: 54881
• UniProt ID: Q9NXF1
• Cytogenetics: 9q31.1
• MW: 105.5 kDa
• Gene Summary: Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit (PubMed:21326211).[UniProtKB/Swiss-Prot Function]