WDR11 (NM_018117) Human Tagged ORF Clone

CAT#: RC205895

WDR11 (Myc-DDK-tagged)-Human WD repeat domain 11 (WDR11)

Product Images

Circular map for RC205895

• 

  Western blot validation of overexpression lysate (Cat# LY413304) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205895 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR11
• Synonyms: BRWD2; DR11; HH14; SRI1; WDR15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205895 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCCCTACACAGTGAACTTCAAGGTGTCGGCGCGCACCCTCACGGGGGCCCTCAACGCCCACAACA
  AGGCGGCGGTGGACTGGGGCTGGCAAGGTTTAATTGCTTATGGATGTCATTCACTTGTGGTAGTGATTGA
  TTCCATTACTGCCCAAACTCTTCAAGTTTTAGAAAAGCATAAAGCTGATGTTGTAAAGGTTAAATGGGCC
  AGGGAAAACTATCACCATAACATTGGCTCACCATATTGCTTACGGTTAGCTTCTGCTGATGTCAATGGGA
  AGATCATCGTCTGGGATGTAGCAGCAGGAGTAGCTCAGTGTGAGATCCAAGAGCATGCCAAGCCTATCCA
  GGATGTTCAGTGGTTGTGGAATCAAGATGCTTCCCGCGATTTACTGCTTGCTATCCACCCGCCAAATTAC
  ATTGTGCTCTGGAATGCCGACACTGGCACCAAACTATGGAAGAAGAGCTATGCAGATAACATTCTTTCTT
  TTTCTTTTGACCCTTTTGATCCCTCACATTTAACTTTGCTTACCAGCGAGGGTATTGTTTTCATCTCAGA
  CTTCTCCCCATCCAAGCCTCCCTCAGGCCCTGGGAAAAAAGTTTACATATCCAGCCCACACTCTAGCCCA
  GCTCATAACAAGCTGGCCACAGCCACAGGTGCCAAGAAAGCTCTAAATAAAGTAAAAATTTTAATCACTC
  AAGAGAAACCTAGTGCTGAATTCATAACTCTCAATGATTGCCTTCAGTTGGCATACCTGCCTTCAAAAAG
  GAATCACATGTTGTTGCTCTATCCTCGAGAGATTTTAATCCTTGACCTTGAGGTGAATCAGACAGTGGGT
  GTGATTGCAATAGAACGCACAGGAGTTCCATTTTTACAGGTAATACCCTGCTTTCAGCGTGATGGTTTAT
  TTTGTCTACATGAAAATGGTTGTATAACTTTACGTGTTCGAAGATCTTATAATAACATTTTTACCACTTC
  AAATGAGGAACCAGATCCAGATCCAGTTCAGGAGCTTACCTATGATTTACGAAGCCAGTGTGATGCAATC
  AGGGTGACAAAAACCGTCCGTCCCTTCAGTATGGTGTGCTGTCCTGTCAATGAGAATGCAGCCGCCCTCG
  TAGTGAGTGATGGCAGGGTCATGATATGGGAACTCAAGTCTGCAGTTTGTAATCGAAATTCACGGAACAG
  TAGTTCTGGTGTGTCACCTTTATATTCACCAGTGTCTTTCTGTGGAATTCCTGTAGGAGTGCTACAGAAT
  AAACTCCCAGACCTTTCCTTAGATAACATGATTGGGCAAAGTGCAATTGCTGGGGAAGAACATCCCAGAG
  GTTCAATTCTGCGGGAAGTGCACCTCAAGTTCCTGCTGACGGGACTGCTTTCAGGACTGCCCGCACCACA
  GTTTGCTATTCGTATGTGTCCACCGTTGACCACAAAAAACATCAAGATGTATCAGCCACTGCTGGCTGTT
  GGTACAAGTAATGGTTCTGTCCTGGTGTACCATCTCACCAGTGGTCTGCTACACAAAGAGTTAAGCATCC
  ACTCATGTGAAGTCAAGGGTATTGAATGGACAAGTTTGACTAGTTTTCTTTCTTTTGCTACCTCAACACC
  AAACAATATGGGATTAGTGAGAAATGAACTTCAACTGGTTGATCTTCCAACAGGTAGGAGCATTGCTTTT
  CGTGGTGAAAGAGGCAATGATGAATCTGCCATCGAAATGATTAAAGTATCTCATTTGAAGCAGTATTTGG
  CAGTCGTATTCAGAGATAAACCCCTGGAGCTATGGGATGTTAGGACTTGTACCCTTCTTAGAGAGATGTC
  CAAAAACTTCCCTACAATAACTGCTTTGGAGTGGTCACCATCTCACAACTTGAAGAGCCTGAGAAAGAAG
  CAACTTGCTACTCGAGAGGCCATGGCCCGCCAGACCGTAGTCTCAGACACAGAGCTGAGTATTGTTGAAT
  CATCTGTGATCAGCTTGCTGCAGGAGGCAGAAAGTAAATCTGAACTTAGTCAGAACATCTCTGCCCGGGA
  ACATTTTGTATTTACCGATATTGATGGCCAAGTGTATCATCTCACTGTTGAAGGAAACTCAGTAAAAGAC
  AGTGCTCGGATTCCACCAGATGGAAGTATGGGTAGTATTACCTGCATCGCTTGGAAAGGTGATACATTAG
  TGCTTGGAGATATGGATGGAAATTTAAATTTCTGGGACTTGAAAGGCAGAGTATCCAGAGGAATACCCAC
  ACACCGAAGTTGGGTGAGGAAGATTCGTTTTGCTCCTGGTAAAGGAAATCAAAAATTAATAGCAATGTAC
  AATGATGGAGCTGAAGTGTGGGATACTAAAGAGGTTCAGATGGTGAGCAGTTTAAGAAGTGGCAGAAATG
  TGACCTTTCGTATATTGGATGTGGACTGGTGTACGTCAGATAAAGTGATCTTGGCCTCAGATGATGGGTG
  CATCAGAGTCCTAGAGATGTCTATGAAGTCTGCGTGCTTTAGAATGGATGAACAAGAGTTAACCGAGCCT
  GTGTGGTGCCCCTATCTCCTTGTTCCAAGGGCCTCTCTTGCCTTGAAAGCCTTCTTATTACACCAGCCTT
  GGAATGGACAGTATTCTTTGGACATTTCTCATGTTGACTATCCAGAAAATGAAGAAATAAAGAATCTCCT
  CCAAGAACAGTTGAATTCATTGTCTAATGACATAAAGAAACTGTTGCTTGATCCAGAATTCACTCTCTTG
  CAGAGGTGCCTGCTTGTTTCAAGGCTCTATGGTGATGAATCGGAGCTGCACTTCTGGACTGTCGCTGCCC
  ACTACCTGCACAGCTTATCCCAGGAAAAGTCAGCCAGCACAACAGCTCCTAAAGAAGCTGCTCCTCGAGA
  CAAACTGAGCAACCCACTGGATATATGCTATGACGTGCTCTGTGAAAATGCCTACTTTCAGAAATTTCAG
  CTAGAAAGGGTTAATCTACAGGAAGTGAAACGGTCAACTTATGATCATACAAGGAAATGTACAGACCAGC
  TACTGCTCTTGGGTCAAACAGACAGAGCTGTGCAGTTGCTGTTGGAAACAAGTGCAGATAACCAGCATTA
  TTACTGTGATTCACTGAAAGCCTGTTTAGTCACTACTGTCACCTCGTCAGGCCCCTCTCAGAGCACCATT
  AAGTTGGTGGCAACGAATATGATTGCCAATGGCAAATTGGCAGAGGGCGTTCAGTTGCTCTGCCTGATAG
  ATAAGGCTGCAGACGCCTGCCGCTACCTGCAGACATACGGCGAGTGGAATCGGGCTGCATGGCTGGCAAA
  AGTCCGTTTGAATCCTGAGGAGTGTGCCGATGTTTTAAGGCGGTGGGTTGACCACCTTTGTTCTCCACAA
  GTCAATCAGAAATCAAAGGCTCTCCTGGTTCTCCTCTCTCTGGGCTGCTTTTTTAGCGTGGCAGAGACGC
  TTCACAGCATGAGATACTTTGATAGAGCAGCCTTATTTGTGGAAGCTTGCCTCAAGTATGGAGCATTTGA
  AGTCACTGAGGACACAGAGAAACTCATCACTGCTATATATGCAGATTATGCCCGGAGTTTGAAGAACCTC
  GGTTTTAAGCAGGGAGCAGTTCTCTTTGCTTCAAAAGCCGGAGCAGCTGGCAAAGACTTATTGAATGAGC
  TTGAGTCCCCCAAGGAAGAACCCATTGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205895 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPYTVNFKVSARTLTGALNAHNKAAVDWGWQGLIAYGCHSLVVVIDSITAQTLQVLEKHKADVVKVKWA
  RENYHHNIGSPYCLRLASADVNGKIIVWDVAAGVAQCEIQEHAKPIQDVQWLWNQDASRDLLLAIHPPNY
  IVLWNADTGTKLWKKSYADNILSFSFDPFDPSHLTLLTSEGIVFISDFSPSKPPSGPGKKVYISSPHSSP
  AHNKLATATGAKKALNKVKILITQEKPSAEFITLNDCLQLAYLPSKRNHMLLLYPREILILDLEVNQTVG
  VIAIERTGVPFLQVIPCFQRDGLFCLHENGCITLRVRRSYNNIFTTSNEEPDPDPVQELTYDLRSQCDAI
  RVTKTVRPFSMVCCPVNENAAALVVSDGRVMIWELKSAVCNRNSRNSSSGVSPLYSPVSFCGIPVGVLQN
  KLPDLSLDNMIGQSAIAGEEHPRGSILREVHLKFLLTGLLSGLPAPQFAIRMCPPLTTKNIKMYQPLLAV
  GTSNGSVLVYHLTSGLLHKELSIHSCEVKGIEWTSLTSFLSFATSTPNNMGLVRNELQLVDLPTGRSIAF
  RGERGNDESAIEMIKVSHLKQYLAVVFRDKPLELWDVRTCTLLREMSKNFPTITALEWSPSHNLKSLRKK
  QLATREAMARQTVVSDTELSIVESSVISLLQEAESKSELSQNISAREHFVFTDIDGQVYHLTVEGNSVKD
  SARIPPDGSMGSITCIAWKGDTLVLGDMDGNLNFWDLKGRVSRGIPTHRSWVRKIRFAPGKGNQKLIAMY
  NDGAEVWDTKEVQMVSSLRSGRNVTFRILDVDWCTSDKVILASDDGCIRVLEMSMKSACFRMDEQELTEP
  VWCPYLLVPRASLALKAFLLHQPWNGQYSLDISHVDYPENEEIKNLLQEQLNSLSNDIKKLLLDPEFTLL
  QRCLLVSRLYGDESELHFWTVAAHYLHSLSQEKSASTTAPKEAAPRDKLSNPLDICYDVLCENAYFQKFQ
  LERVNLQEVKRSTYDHTRKCTDQLLLLGQTDRAVQLLLETSADNQHYYCDSLKACLVTTVTSSGPSQSTI
  KLVATNMIANGKLAEGVQLLCLIDKAADACRYLQTYGEWNRAAWLAKVRLNPEECADVLRRWVDHLCSPQ
  VNQKSKALLVLLSLGCFFSVAETLHSMRYFDRAALFVEACLKYGAFEVTEDTEKLITAIYADYARSLKNL
  GFKQGAVLFASKAGAAGKDLLNELESPKEEPIEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018117
• ORF Size: 3672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018117.9
• RefSeq Size: 4746 bp
• RefSeq ORF: 3675 bp
• Locus ID: 55717
• UniProt ID: Q9BZH6
• Cytogenetics: 10q26.12
• Domains: WD40
• MW: 136.7 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene is located in the chromosome 10q25-26 region, which is frequently deleted in gliomas and tumors of other tissues, and is disrupted by the t(10;19) translocation rearrangement in glioblastoma cells. The gene location suggests that it is a candidate gene for the tumor suppressor locus. [provided by RefSeq, Jul 2008]