ST3GAL6 (NM_006100) Human Tagged ORF Clone

CAT#: RC205750

ST3GAL6 (Myc-DDK-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 6 (ST3GAL6)

Product Images

Circular map for RC205750

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  Western blot validation of overexpression lysate (Cat# LY416864) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205750 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ST3GAL6 protein (Cat# TP305750). The protein was produced from HEK293T cells transfected with ST3GAL6 cDNA clone (Cat# RC205750) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ST3GAL6
• Synonyms: SIAT10; ST3GALVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205750 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGGGTATCTTGTGGCCATATTCCTGAGTGCTGTCTTCCTCTATTATGTACTGCATTGCATATTAT
  GGGGAACGAATGTCTATTGGGTGGCACCTGTGGAAATGAAACGGAGAAATAAGATCCAGCCTTGTTTATC
  AAAGCCAGCTTTTGCCTCTCTGCTGAGGTTTCATCAGTTTCACCCTTTTCTGTGTGCGGCTGATTTTAGA
  AAGATTGCTTCCTTGTATGGTAGCGATAAGTTTGATTTGCCCTATGGGATGAGAACATCAGCGGAATATT
  TTCGACTTGCTCTTTCAAAACTGCAGAGTTGTGATCTCTTTGATGAGTTTGACAACATACCCTGTAAAAA
  GTGTGTGGTGGTTGGTAATGGAGGAGTTTTGAAGAATAAGACATTAGGAGAAAAAATCGACTCCTATGAT
  GTAATAATAAGAATGAATAATGGTCCTGTTTTAGGACATGAAGAAGAAGTTGGGAGAAGGACAACCTTCC
  GACTTTTTTATCCAGAATCTGTTTTTTCAGATCCTATTCACAATGACCCTAATACGACAGTGATTCTCAC
  TGCTTTTAAGCCACATGATTTAAGGTGGCTGTTGGAATTGTTGATGGGTGACAAAATAAACACTAATGGT
  TTTTGGAAGAAACCAGCCTTAAACCTGATTTATAAACCTTATCAAATCCGAATATTAGATCCTTTCATTA
  TCAGAACAGCAGCTTATGAACTGCTTCATTTTCCAAAAGTGTTTCCCAAAAATCAGAAACCTAAACACCC
  AACAACAGGAATTATTGCCATCACATTGGCGTTTTACATATGTCACGAAGTTCACCTAGCTGGTTTTAAA
  TACAACTTTTCTGACCTCAAGAGTCCTTTGCACTACTATGGGAATGCCACCATGTCTTTGATGAATAAGA
  ACGCGTATCACAATGTGACTGCAGAGCAGCTCTTTTTGAAGGACATTATAGAAAAAAACCTCGTAATCAA
  CTTGACTCAAGAT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205750 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRGYLVAIFLSAVFLYYVLHCILWGTNVYWVAPVEMKRRNKIQPCLSKPAFASLLRFHQFHPFLCAADFR
  KIASLYGSDKFDLPYGMRTSAEYFRLALSKLQSCDLFDEFDNIPCKKCVVVGNGGVLKNKTLGEKIDSYD
  VIIRMNNGPVLGHEEEVGRRTTFRLFYPESVFSDPIHNDPNTTVILTAFKPHDLRWLLELLMGDKINTNG
  FWKKPALNLIYKPYQIRILDPFIIRTAAYELLHFPKVFPKNQKPKHPTTGIIAITLAFYICHEVHLAGFK
  YNFSDLKSPLHYYGNATMSLMNKNAYHNVTAEQLFLKDIIEKNLVINLTQD
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006100
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006100.3
• RefSeq Size: 3571 bp
• RefSeq ORF: 996 bp
• Locus ID: 10402
• UniProt ID: Q9Y274
• Cytogenetics: 3q12.1
• Domains: Glyco_transf_29
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• MW: 38.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the sialyltransferase family. Members of this family are enzymes that transfer sialic acid from the activated cytidine 5'-monophospho-N-acetylneuraminic acid to terminal positions on sialylated glycolipids (gangliosides) or to the N- or O-linked sugar chains of glycoproteins. This protein has high specificity for neolactotetraosylceramide and neolactohexaosylceramide as glycolipid substrates and may contribute to the formation of selectin ligands and sialyl Lewis X, a carbohydrate important for cell-to-cell recognition and a blood group antigen. [provided by RefSeq, Apr 2016]