CDC25C (NM_001790) Human Tagged ORF Clone

CAT#: RC205641

CDC25C (Myc-DDK-tagged)-Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 1

Product Images

Circular map for RC205641

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  Western blot validation of overexpression lysate (Cat# LY419747) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205641 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDC25C protein (Cat# TP305641). The protein was produced from HEK293T cells transfected with CDC25C cDNA clone (Cat# RC205641) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC25C
• Synonyms: CDC25; PPP1R60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205641 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTA
  ATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTAG
  AACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCCCAAAATGT
  TGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTCAGCTTACCACTTCTGCAGACCTTG
  ATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGACCAGCA
  CCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAG
  AGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGACAGTGAAATGA
  AATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGGAGAAGACCAGGC
  AGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAGGTGAGCAGAAGTGGC
  CTATATCGCTCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGG
  ACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATG
  TTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGG
  CACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGAAGT
  ATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAGGGTCTGATTGAGAAGTTTTATGT
  CATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTCAG
  GAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCG
  TGTTCCACTGTGAATTCTCCTCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTC
  TCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTCTTT
  CCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAGT
  TGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCT
  GGTGAAGGACATGAGCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205641 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKC
  CLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRK
  RDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSG
  LYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQG
  HLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQ
  EELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFF
  PEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001790
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001790.2, NP_001781.1
• RefSeq Size: 2191 bp
• RefSeq ORF: 1422 bp
• Locus ID: 995
• UniProt ID: P30307
• Cytogenetics: 5q31.2
• Domains: RHOD
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• MW: 53.3 kDa
• Gene Summary: This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]