CDC25C (NM_001790) Human Untagged Clone

CAT#: SC109054

CDC25C (untagged)-Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC25C
• Synonyms: CDC25; PPP1R60
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109054 sequence for NM_001790 edited (data generated by NextGen Sequencing)
  ATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGT
  TTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACC
  GTCTGTCCAGATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTA
  AGCATTTTGTCTGGAGGAACCCCAAAACGTTGCCTCGATCTTTCGAATCTTAGCAGTGGG
  GAGATAACTGCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGAT
  TCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGACCAGCACCTAATGAAA
  TGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAG
  AGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGAC
  AGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCA
  AACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAA
  GATCAAGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAAC
  TTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAA
  GTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAG
  ACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGG
  CACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAA
  GATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAGGGT
  CTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACAC
  ATCCAGGGAGCCTTAAACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAG
  CCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCC
  TCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAG
  TATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTCTTT
  CCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCAC
  AAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTG
  CGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGA
  
  Clone variation with respect to NM_001790.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001790 unedited
  CCGTCAGAAGTGTATACGACTCATATAGGCGGCCGCGNAATCGGCACGAGGCTTTGGCCT
  TCTGCTCAGGGAAGACTCTGAGTCCGACGTTGGCCTACCCAGTCGGAAGGCAGAGCTGCA
  ATCTAGTTAACTACCTCCTTTCCCCTAGATTTCCTTTCATTCTGCTCAAGTCTTCGCCTG
  TGTCCGATCCCTATCTACTTTCTCTCCTCTTGTAGGCAAGCCTCAGACTCCAGGCTTGAG
  CTAGGTTTTGTTTTTCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCAT
  CCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAA
  TGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTAGAA
  CTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCC
  CAAAACGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTCAGCTTA
  CCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGC
  ATTTAGCTGGGATGAATCATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTT
  GTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTTCAT
  CTGCAAATAAAGAAAATGACAATGGAAACTTGNTGGACAGTGAAATGAAATATTTGGGCA
  GTCCCATTACTACTGNTCCCAAATTGGATAGAAATCCAAACCTAGGAGAAGACCAGGCAG
  AAGAGATTTCAGATGAATTATGGGAGTTTTCCCTTGAAGATCAAGAGCCACAGTGAGCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001790 unedited
  TATGGCCGCGGCCGCAATCTAGAGTCGAGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAAAAACATCTTTTAATAATCTTGGGGTTG
  GCCCTCCAAAAGGCCCCTTTTTGCTCAAGGGTTTTGCAGGGGCTTTTGGGGACTTGTTAA
  CAACCCAGGGCTGGAAAGGTATCATGGGCTCATGTCCTTCACCAAAAGGGGAATTTGGTC
  CCCCAAGTGGCGGTCCCCTTTCTGGACTTTGGTTTGGGTTCGACACCCCAACAACTCAAT
  CTTGGGGGCCCGAAGAAGCATAAGGCAAAAACTCTGGGGGTCACACAGTTCCCTATATTC
  TGGAAAAAAATCTCTGTAACCCCCTTTAAAGAAATATAACTCTGGGGAAAACAAAGCAGG
  ATACTGGGTCAAAAACCTGTCCTTTTTACCCAAACAGGGGGACATTTGGGGGCCCCTCTC
  TGAGGAGAATTCACAGGGGAACACCATGATTATTCTCTTCTGGGGGGCCAAAGGGACCAA
  GGGCTTCTTCAAAAAGAAATTAAACAGGTCTTCCTGACTATATAAAGTTAAAGCTCCCTG
  GAAGGGGCCCCCCCGAACCCATATGGGTAGCGACCATCAATGACCTAAAACTTTTCAATC
  AAACCCTGGAACTTCCCCCACAGTAAAGCAACCCCCTGGTTCTGGGTTGACATACTTCAG
  AACTTGGTGTTTCCCTGACACGGTTGGCAGCGCACATACCTTTGAAAAAACACCCATCAA
  GTGCCCCTGGTTAAAAACCTCCTCCCACCTCTGAGTGAAAGTATGCCCCCACAGAGACTG
  CCTTCTTTAACACTAACCCTTCCTGACCTTCCCTTGGCCAAAAAAAACTTTTTTTTACTT
  A
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001790
• Insert Size: 1860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001790.2, NP_001781.1
• RefSeq Size: 2115 bp
• RefSeq ORF: 1422 bp
• Locus ID: 995
• UniProt ID: P30307
• Cytogenetics: 5q31.2
• Domains: RHOD
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) encodes isoform a. Variants 1 and 3 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.