SENP5 (NM_152699) Human Tagged ORF Clone

CAT#: RC205592

SENP5 (Myc-DDK-tagged)-Human SUMO1/sentrin specific peptidase 5 (SENP5)

Product Images

Circular map for RC205592

• 

  Western blot validation of overexpression lysate (Cat# LY403484) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205592 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SENP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205592 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAACAGAGGAAAATTCTATGGAGGAAAGGAATCCACTTAGCCTTTTCTGAGAAATGGAATACTG
  GGTTTGGAGGCTTTAAGAAGTTTTATTTTCACCAACACTTGTGCATTCTGAAAGCTAAGCTGGGAAGGCC
  AGTTACTTGGAATAGACAGTTGAGACATTTCCAGGGTAGAAAGAAAGCTCTTCAAATCCAGAAAACGTGG
  ATCAAGGATGAACACCTTTGTGCTAAGACCAAGTTCAATGTGGCTACTCAAAATGTTAGTACTTTGTCCT
  CTAAAGTGAAAAGAAAGGACGCTAAACACTTCATTTCCTCCTCAAAGACTCTCCTGAGACTCCAAGCAGA
  GAAGCTGTTGTCATCAGCAAAGAATTCTGACCATGAATACTGCAGAGAGAAAAATCTCTTGAAGGCAGTT
  ACTGACTTTCCATCAAATAGTGCTTTAGGTCAGGCCAATGGTCACAGACCTAGGACAGACCCACAACCTT
  CTGACTTTCCCATGAAGTTCAATGGGGAGAGCCAAAGTCCAGGTGAGAGTGGCACGATTGTGGTCACCTT
  GAACAACCATAAGAGAAAGGGCTTTTGTTACGGCTGCTGCCAAGGGCCGGAGCACCACAGGAATGGGGGA
  CCCTTGATTCCAAAAAAGTTCCAACTTAACCAACATAGAAGGATAAAATTATCTCCTCTTATGATGTATG
  AGAAATTATCCATGATTAGATTTCGGTACAGGATTCTCAGATCCCAGCACTTCAGAACCAAAAGCAAGGT
  TTGCAAGCTAAGAAAAGCCCAGCGAAGCTGGGTACAGAAAGTCACTGGGGACCATCAAGAGACCCGTAGG
  GAGAACGGTGAGGGTGGCAGTTGCAGCCCATTTCCTTCCCCAGAACCTAAAGACCCTTCTTGTCGGCATC
  AGCCGTACTTTCCAGATATGGACAGCAGTGCTGTGGTGAAGGGGACGAACTCTCATGTGCCTGATTGCCA
  CACTAAAGGAAGCTCTTTCTTGGGCAAGGAGCTTAGTTTAGACGAAGCATTCCCTGACCAACAGAATGGC
  AGTGCCACAAACGCCTGGGACCAGTCATCCTGTTCTTCTCCTAAGTGGGAGTGTACAGAGCTGATTCATG
  ACATCCCCTTACCAGAACATCGTTCTAATACCATGTTCATTTCAGAAACTGAAAGAGAAATTATGACTCT
  GGGTCAGGAAAATCAGACAAGTTCTGTCAGTGATGACAGAGTAAAACTGTCAGTGTCTGGAGCAGATACA
  TCTGTGAGTAGCGTAGATGGGCCTGTGTCCCAAAAGGCTGTTCAAAATGAGAACTCATACCAGATGGAGG
  AGGATGGATCTCTCAAGCAGAGCATTCTTAGTTCTGAGTTGCTGGACCACCCTTACTGTAAAAGTCCACT
  GGAGGCTCCCTTGGTGTGCAGTGGACTCAAACTAGAAAATCAAGTAGGAGGTGGAAAGAACAGTCAGAAA
  GCCTCTCCAGTGGATGATGAACAGCTGTCAGTCTGTCTTTCTGGATTCCTAGATGAGGTTATGAAGAAGT
  ATGGCAGTTTGGTTCCACTCAGTGAAAAAGAAGTCCTTGGAAGATTAAAAGATGTCTTTAATGAAGACTT
  TTCTAATAGAAAACCATTTATCAATAGGGAAATAACAAACTATCGGGCCAGACATCAAAAATGTAACTTC
  CGTATCTTCTATAATAAACACATGCTGGATATGGACGACCTGGCGACTCTGGATGGTCAGAACTGGCTGA
  ATGACCAGGTCATTAATATGTATGGTGAGCTGATAATGGATGCAGTCCCAGACAAAGTTCACTTCTTCAA
  CAGCTTTTTTCATAGACAGCTGGTAACCAAAGGATATAATGGAGTAAAAAGATGGACTAAAAAGGTGGAT
  TTGTTTAAAAAGAGTCTTCTGTTGATTCCTATTCACCTGGAAGTCCACTGGTCTCTCATTACTGTGACAC
  TCTCTAATCGAATTATTTCATTTTATGATTCCCAAGGCATTCATTTTAAGTTTTGTGTAGAGAATATAAG
  AAAGTATTTGCTGACTGAAGCCAGAGAAAAAAATAGACCTGAATTTCTTCAGGGTTGGCAGACTGCTGTT
  ACGAAGTGTATTCCACAACAGAAAAACGACAGTGACTGTGGAGTCTTTGTGCTCCAGTACTGCAAGTGCC
  TCGCCTTAGAGCAGCCTTTCCAGTTTTCACAAGAAGACATGCCCCGAGTGCGGAAGAGGATTTACAAGGA
  GCTATGTGAGTGCCGGCTCATGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205592 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKQRKILWRKGIHLAFSEKWNTGFGGFKKFYFHQHLCILKAKLGRPVTWNRQLRHFQGRKKALQIQKTW
  IKDEHLCAKTKFNVATQNVSTLSSKVKRKDAKHFISSSKTLLRLQAEKLLSSAKNSDHEYCREKNLLKAV
  TDFPSNSALGQANGHRPRTDPQPSDFPMKFNGESQSPGESGTIVVTLNNHKRKGFCYGCCQGPEHHRNGG
  PLIPKKFQLNQHRRIKLSPLMMYEKLSMIRFRYRILRSQHFRTKSKVCKLRKAQRSWVQKVTGDHQETRR
  ENGEGGSCSPFPSPEPKDPSCRHQPYFPDMDSSAVVKGTNSHVPDCHTKGSSFLGKELSLDEAFPDQQNG
  SATNAWDQSSCSSPKWECTELIHDIPLPEHRSNTMFISETEREIMTLGQENQTSSVSDDRVKLSVSGADT
  SVSSVDGPVSQKAVQNENSYQMEEDGSLKQSILSSELLDHPYCKSPLEAPLVCSGLKLENQVGGGKNSQK
  ASPVDDEQLSVCLSGFLDEVMKKYGSLVPLSEKEVLGRLKDVFNEDFSNRKPFINREITNYRARHQKCNF
  RIFYNKHMLDMDDLATLDGQNWLNDQVINMYGELIMDAVPDKVHFFNSFFHRQLVTKGYNGVKRWTKKVD
  LFKKSLLLIPIHLEVHWSLITVTLSNRIISFYDSQGIHFKFCVENIRKYLLTEAREKNRPEFLQGWQTAV
  TKCIPQQKNDSDCGVFVLQYCKCLALEQPFQFSQEDMPRVRKRIYKELCECRLMD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152699
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152699.3
• RefSeq Size: 6324 bp
• RefSeq ORF: 2268 bp
• Locus ID: 205564
• UniProt ID: Q96HI0
• Cytogenetics: 3q29
• Protein Families: Druggable Genome, Protease
• MW: 86.7 kDa
• Gene Summary: The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP5, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM, Jun 2009]