FEM 1C (FEM1C) (NM_020177) Human Tagged ORF Clone

CAT#: RC205563

FEM1C (Myc-DDK-tagged)-Human fem-1 homolog c (C. elegans) (FEM1C)

Product Images

Circular map for RC205563

• 

  Western blot validation of overexpression lysate (Cat# LY412600) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205563 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FEM 1C
• Synonyms: EUROIMAGE686608; EUROIMAGE783647; FEM1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205563 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTAAAGACAGCAGTATTTAACGCAGCTCGGGATGGCAAACTCCGGCTTCTCACCAAATTGTTGG
  CAAGCAAATCCAAAGAGGAGGTTTCCTCCTTGATCTCTGAAAAAACAAATGGGGCCACGCCACTCTTGAT
  GGCCGCCAGGTATGGGCACCTTGACATGGTGGAATTCCTCCTAGAGCAATGCAGTGCCTCCATAGAAGTT
  GGGGGCTCCGTCAATTTTGATGGCGAAACCATTGAGGGGGCTCCCCCTTTATGGGCCGCTTCTGCAGCAG
  GACATCTGAAGGTGGTCCAGTCCTTGTTAAATCATGGAGCATCTGTCAACAACACGACTTTAACCAATTC
  AACTCCTCTTCGAGCTGCGTGTTTCGATGGCCATTTGGAAATAGTGAAGTACCTTGTAGAACACAAAGCT
  GATTTGGAAGTGTCAAACCGACATGGGCATACGTGCTTGATGATTTCATGTTACAAAGGACATAAAGAGA
  TTGCTCAGTATTTACTTGAAAAGGGGGCAGATGTTAATAGAAAAAGTGTCAAAGGTAATACTGCATTGCA
  TGATTGTGCAGAATCTGGAAGTTTGGACATCATGAAGATGCTTCTTATGTATTGTGCCAAGATGGAAAAG
  GATGGTTATGGAATGACTCCCCTTCTCTCAGCAAGTGTGACTGGTCACACAAATATTGTGGATTTTCTGA
  CACACCATGCACAGACCAGCAAGACAGAACGTATTAATGCTCTAGAGCTTCTGGGAGCTACATTTGTAGA
  CAAAAAAAGAGATCTGCTTGGGGCTTTGAAATACTGGAAAAAGGCAATGAACATGAGGTACAGTGATAGG
  ACTAATATTATTAGTAAACCAGTGCCACAGACACTAATAATGGCTTATGATTATGCCAAGGAAGTGAACA
  GTGCAGAAGAGCTAGAAGGTCTTATTGCTGATCCTGATGAGATGAGAATGCAGGCACTATTAATCAGAGA
  ACGTATTCTTGGTCCTTCTCATCCTGATACCTCTTACTATATTAGATATAGAGGCGCTGTCTATGCAGAC
  TCTGGAAATTTCAAACGATGCATCAACCTATGGAAGTATGCTTTGGATATGCAGCAGAGCAATTTGGATC
  CTTTAAGCCCAATGACAGCCAGCAGCTTATTATCTTTTGCAGAACTATTCTCCTTTATGCTACAGGATAG
  GGCTAAAGGCCTGCTGGGTACTACTGTTACATTTGATGATCTTATGGGCATACTTTGCAAAAGCGTCCTT
  GAAATAGAGCGAGCTATCAAACAAACTCAGTGTCCAGCTGACCCATTACAGTTAAATAAGGCCCTTTCTA
  TTATTTTGCACTTAATTTGCTTGTTAGAGAAAGTTCCTTGTACTCTAGAACAAGACCATTTCAAAAAGCA
  GACTATATACAGGTTTCTTAAGCTGCATCCAAGGGGAAAGAATAACTTCAGCCCTCTTCATCTGGCTGTG
  GACAAGAATACTACATGTGTAGGGCGGTACCCTGTTTGTAAATTTCCATCTCTACAAGTTACTGCAATAC
  TGATAGAATGTGGTGCTGATGTGAACGTCAGAGACTCGGATGACAACAGTCCCCTGCATATCGCTGCTCT
  TAACAACCATCCAGACATCATGAATCTCCTTATTAAATCAGGTGCACATTTTGATGCCACAAACTTGCAC
  AAACAAACTGCTAGTGACTTGCTGGATGAGAAGGAAATAGCTAAAAATTTAATCCAGCCTATAAATCATA
  CCACATTGCAGTGTCTTGCTGCTCGTGTCATAGTGAATCATAGAATATATTATAAAGGGCATATCCCAGA
  AAAGCTAGAGACTTTTGTTTCCCTTCATAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205563 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLKTAVFNAARDGKLRLLTKLLASKSKEEVSSLISEKTNGATPLLMAARYGHLDMVEFLLEQCSASIEV
  GGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLNHGASVNNTTLTNSTPLRAACFDGHLEIVKYLVEHKA
  DLEVSNRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLDIMKMLLMYCAKMEK
  DGYGMTPLLSASVTGHTNIVDFLTHHAQTSKTERINALELLGATFVDKKRDLLGALKYWKKAMNMRYSDR
  TNIISKPVPQTLIMAYDYAKEVNSAEELEGLIADPDEMRMQALLIRERILGPSHPDTSYYIRYRGAVYAD
  SGNFKRCINLWKYALDMQQSNLDPLSPMTASSLLSFAELFSFMLQDRAKGLLGTTVTFDDLMGILCKSVL
  EIERAIKQTQCPADPLQLNKALSIILHLICLLEKVPCTLEQDHFKKQTIYRFLKLHPRGKNNFSPLHLAV
  DKNTTCVGRYPVCKFPSLQVTAILIECGADVNVRDSDDNSPLHIAALNNHPDIMNLLIKSGAHFDATNLH
  KQTASDLLDEKEIAKNLIQPINHTTLQCLAARVIVNHRIYYKGHIPEKLETFVSLHR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020177
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020177.3
• RefSeq Size: 5813 bp
• RefSeq ORF: 1854 bp
• Locus ID: 56929
• UniProt ID: Q96JP0
• Cytogenetics: 5q22.3
• Domains: ANK
• MW: 68.7 kDa
• Gene Summary: Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.[UniProtKB/Swiss-Prot Function]