DDHD1 (NM_030637) Human Tagged ORF Clone

CAT#: RC205548

DDHD1 (Myc-DDK-tagged)-Human DDHD domain containing 1 (DDHD1), transcript variant 1

Product Images

Circular map for RC205548

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  Western blot validation of overexpression lysate (Cat# LY410760) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205548 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DDHD1 protein (Cat# TP305548). The protein was produced from HEK293T cells transfected with DDHD1 cDNA clone (Cat# RC205548) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDHD1
• Synonyms: iPLA1alpha; PA-PLA1; PAPLA1; SPG28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205548 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACCCGGGCCGCGGGTCCCCACGGAGCCCCGAGCATAACGGCCGAGGCGGCGGCGGCGGCGCCT
  GGGAGCTGGGCTCAGACGCGAGGCCAGCGTTCGGCGGCGGCGTCTGCTGCTTCGAGCACCTGCCCGGCGG
  GGACCCGGACGACGGCGACGTGCCCCTGGCCCTGCTGCGCGGGGAACCCGGGCTGCATTTGGCGCCGGGC
  ACCGACGACCACAACCACCACCTCGCGCTGGACCCCTGCCTCAGTGACGAGAACTATGACTTCAGCTCCG
  CCGAGTCGGGCTCCTCGCTGCGCTACTACAGCGAGGGTGAGAGCGGCGGCGGCGGCAGCTCCTTGTCGCT
  GCACCCGCCGCAGCAGCCTCCGCTGGTCCCGACGAACTCGGGGGGCGGCGGCGCGACAGGAGGGTCCCCC
  GGGGAAAGGAAACGTACCCGGCTTGGCGGCCCGGCGGCCCGGCACCGCTATGAGGTAGTGACGGAGCTGG
  GCCCGGAGGAGGTACGCTGGTTCTACAAGGAGGACAAGAAGACCTGGAAGCCCTTCATCGGCTACGACTC
  GCTCCGCATCGAGCTCGCCTTCCGGACCCTGCTGCAGACCACGGGTGCCCGGCCCCAGGGCGGGGACCGG
  GACGGCGACCATGTGTGCTCCCCCACGGGCCCAGCCTCCAGTTCCGGAGAAGATGACGATGAGGACCGCG
  CCTGCGGCTTCTGCCAGAGTACGACGGGGCACGAGCCGGAGATGGTGGAGCTTGTGAACATCGAGCCTGT
  GTGCGTGCGGGGCGGCCTCTACGAGGTGGATGTGACCCAAGGAGAGTGCTACCCGGTGTACTGGAACCAG
  GCTGATAAAATACCAGTAATGCGTGGACAGTGGTTTATTGACGGCACTTGGCAGCCTCTAGAAGAGGAAG
  AAAGTAATTTAATTGAGCAAGAACATCTCAATTGTTTTAGGGGCCAGCAGATGCAGGAAAATTTCGATAT
  TGAAGTGTCAAAATCCATAGATGGAAAAGATGCTGTTCATAGTTTCAAGTTGAGTCGAAACCATGTGGAC
  TGGCACAGTGTGGATGAAGTATATCTTTATAGTGATGCAACAACATCTAAAATTGCAAGAACAGTTACCC
  AAAAACTGGGATTTTCTAAAGCATCAAGTAGTGGTACCAGACTTCATAGAGGTTATGTAGAAGAAGCCAC
  ATTAGAAGACAAGCCATCACAGACTACCCATATTGTATTTGTTGTGCATGGCATTGGGCAGAAAATGGAC
  CAAGGAAGAATTATCAAAAATACAGCTATGATGAGAGAAGCTGCAAGAAAAATAGAAGAAAGGCATTTTT
  CCAACCATGCAACACATGTTGAATTTCTGCCTGTTGAGTGGCGGTCAAAACTTACTCTTGATGGAGACAC
  TGTTGATTCCATTACTCCTGACAAAGTACGAGGTTTAAGGGATATGCTGAACAGCAGTGCAATGGACATA
  ATGTATTATACTAGTCCACTTTATAGAGATGAACTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTGT
  ATTCCCTTTTCTGTTCTCGGAATCCAGACTTTGAAGAAAAAGGGGGTAAAGTCTCAATAGTATCACATTC
  CTTGGGATGTGTAATTACTTATGACATAATGACTGGCTGGAATCCAGTTCGGCTGTATGAACAGTTGCTG
  CAAAAGGAAGAAGAGTTGCCTGATGAACGATGGATGAGCTATGAAGAACGACATCTTCTTGATGAACTCT
  ATATAACTAAACGACGGCTGAAGGAAATAGAAGAACGGCTTCACGGATTGAAAGCATCATCTATGACACA
  AACACCTGCCTTAAAATTTAAGGTTGAGAATTTCTTCTGTATGGGATCCCCATTAGCAGTTTTCTTGGCG
  TTGCGTGGCATCCGCCCAGGAAATACTGGAAGTCAAGACCATATTTTGCCTAGAGAGATTTGTAACCGGT
  TACTAAATATTTTTCATCCTACAGATCCAGTGGCTTATAGATTAGAACCATTAATACTGAAACGCTACAG
  CAACATTTCACCTGTCCAGATCCACTGGTACAATACTTCAAATCCTTTACCTTATGAACATATGAAGCCA
  AGCTTTCTCAACCCAGCTAAAGAACCTACCTCAGTTTCAGAGAATGAAGGCATTTCAACCATACCAAGCC
  CTGTGACCTCACCAGTTTTGTCCCGCCGACACTATGGAGAATCTATAACAAATATAGGCAAAGCAAGCAT
  ATTAGGGGCTGCTAGCATTGGAAAGGGACTTGGAGGAATGTTGTTCTCAAGATTTGGACGTTCATCTACA
  ACACAGTCATCTGAAACATCAAAAGACTCAATGGAAGATGAGAAGAAGCCAGTTGCCTCACCTTCTGCTA
  CCACCGTAGGGACACAGACCCTTCCACATAGCAGTTCTGGCTTCCTCGATTCTGCATTGGAGTTGGATCA
  CAGGATTGATTTTGAACTCAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTCAGCTGTCACGTCGCATACT
  GCCTATTGGTCATCCTTGGATGTTGCCCTTTTTCTTTTAACCTTCATGTATAAACATGAGCACGATGATG
  ATGCAAAACCCAATTTAGATCCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205548 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNYPGRGSPRSPEHNGRGGGGGAWELGSDARPAFGGGVCCFEHLPGGDPDDGDVPLALLRGEPGLHLAPG
  TDDHNHHLALDPCLSDENYDFSSAESGSSLRYYSEGESGGGGSSLSLHPPQQPPLVPTNSGGGGATGGSP
  GERKRTRLGGPAARHRYEVVTELGPEEVRWFYKEDKKTWKPFIGYDSLRIELAFRTLLQTTGARPQGGDR
  DGDHVCSPTGPASSSGEDDDEDRACGFCQSTTGHEPEMVELVNIEPVCVRGGLYEVDVTQGECYPVYWNQ
  ADKIPVMRGQWFIDGTWQPLEEEESNLIEQEHLNCFRGQQMQENFDIEVSKSIDGKDAVHSFKLSRNHVD
  WHSVDEVYLYSDATTSKIARTVTQKLGFSKASSSGTRLHRGYVEEATLEDKPSQTTHIVFVVHGIGQKMD
  QGRIIKNTAMMREAARKIEERHFSNHATHVEFLPVEWRSKLTLDGDTVDSITPDKVRGLRDMLNSSAMDI
  MYYTSPLYRDELVKGLQQELNRLYSLFCSRNPDFEEKGGKVSIVSHSLGCVITYDIMTGWNPVRLYEQLL
  QKEEELPDERWMSYEERHLLDELYITKRRLKEIEERLHGLKASSMTQTPALKFKVENFFCMGSPLAVFLA
  LRGIRPGNTGSQDHILPREICNRLLNIFHPTDPVAYRLEPLILKRYSNISPVQIHWYNTSNPLPYEHMKP
  SFLNPAKEPTSVSENEGISTIPSPVTSPVLSRRHYGESITNIGKASILGAASIGKGLGGMLFSRFGRSST
  TQSSETSKDSMEDEKKPVASPSATTVGTQTLPHSSSGFLDSALELDHRIDFELREGLVESRYWSAVTSHT
  AYWSSLDVALFLLTFMYKHEHDDDAKPNLDPI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030637
• ORF Size: 2616 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030637.1, NP_085140.1
• RefSeq Size: 12885 bp
• RefSeq ORF: 2619 bp
• Locus ID: 80821
• UniProt ID: Q8NEL9
• Cytogenetics: 14q22.1
• MW: 97.1 kDa
• Gene Summary: This gene is a member of the intracellular phospholipase A1 gene family. The protein encoded by this gene preferentially hydrolyzes phosphatidic acid. It is a cytosolic protein with some mitochondrial localization, and is thought to be involved in the regulation of mitochondrial dynamics. Overexpression of this gene causes fragmentation of the tubular structures in mitochondria, while depletion of the gene results in mitochondrial tubule elongation. Deletion of this gene in male mice caused fertility defects, resulting from disruption in the organization of the mitochondria during spermiogenesis. In humans, mutations in this gene have been associated with hereditary spastic paraplegia (HSP), also known as Strumpell-Lorrain disease, or, familial spastic paraparesis (FSP). This inherited disorder is characterized by progressive weakness and spasticity of the legs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]