SPATA16 (NM_031955) Human Tagged ORF Clone

CAT#: RC205462

SPATA16 (Myc-DDK-tagged)-Human spermatogenesis associated 16 (SPATA16)

Product Images

Circular map for RC205462

• 

  Western blot validation of overexpression lysate (Cat# LY403133) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205462 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPATA16
• Synonyms: NYD-SP12; SPGF6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205462 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCAGGAAGCAGTAGGAGTTTGGAGAATGCAGTGAATAGGATCTATCATGATCAGCTTGTTCCAA
  AGATAAACACAAGCAAGAAAATGTCCACCTTAGCGCACCCACCTAACATCCTGGAAATGTCACAAGAGAT
  TAAGAAAAACTGTGGAGGTAAACAGGTAGAAATCACACTTGAAAGAACAAAAATGACAAAGGGCATCAAA
  GAAAAACAAAGCAATGATTTAGAGAAAGCAGCCTTTAAACGGAAGGCAGAAGGTGAAGGAAAGCCAACTA
  GAAAGAAACAGGCAAAGATAACAGAACTTGACAATCAGTTAATCACCATGCCTCTGCCTCACATCCCCTT
  AAAGAACATAATGGATGTGGAAATGAAGTTGGTCTACATTGATGAAATGGGTGTTCGCTATGAGTTTGTA
  GAGTCCTTCATGTCTACTGGGAGTCAGCCAACATGCCAAGCTGCTGAAATAGTAGACCCTTTGAGTGTAC
  ATAATTTCAGCTTTCTGCCTCAGATTGACAAATGGCTTCAGGTAGCCTTAAAGGATGCCAGCTCTTGCTA
  TAGACAAAAGAAATACGCCTTGGCAGCAGGACAGTTCAGAACAGCACTTGAGCTTTGCAGCAAAGGAGCA
  GTTCTGGGAGAACCATTTGATGCACCTGCTGAAGATATAGCAAGCGTGGCAAGTTTCATTGAGACAAAGC
  TTGTTACCTGTTATCTACGGATGAGGAAACCAGATCTTGCCCTGAATCATGCACACAGGAGCATTGTTTT
  AAACCCAGCCTATTTCCGGAATCATCTTCGTCAAGCAACAGTGTTTAGATGTCTGGAGAGGTATTCAGAG
  GCTGCCCGGAGTGCCATGATTGCTGACTACATGTTCTGGCTTGGTGGAGGAAGGGAAGAGAGCATAAGCA
  AGCTCATCAAACTGTATTGGCAGGCCATGATTGAGGAAGCCATCACCAGAGCTGAATCTTTCTCGGTTAT
  GTACACACCATTTGCGACAAAAATAAGAGCTGATAAAATAGAAAAAGTAAAAGATGCTTTCACAAAAACT
  CATCCTGCATATGCAGAGTATATGTACACAGATCTTCAAGCACTCCACATGTTGCCTCAGACAGTTGACT
  GGTCATCTTTTCCTCCCCAACAATATCTCTTGACTCTTGGGTTCAAAAACAAAGATGATGGAAAATTTTT
  GGAAAAAATATCAAGCAGGAAGCTGCCGATATTCACAGAGCATAAAACACCTTTCGGTCTGACCAGAGAA
  GATACAGTGAGGCAAATGGAGACAATGGGGAAGCGAATCTTGCCAATATTGGATTTTATTAGAAGCACCC
  AATTGAATGGGAGTTTTCCTGCATCCTCAGGTGTGATGGAGAAGTTGCAATATGCCAGCCTCCTCAGCCA
  GCTGCAGAGAGTAAAGGAGCAGTCCCAGGTGATTAATCAAGCAATGGCAGAGCTAGCAACCATTCCCTAC
  CTACAGGACATCAGTCAACAGGAGGCAGAATTGCTGCAGTCACTAATGGCAGATGCTATGGACACCCTTG
  AAGGAAGAAGAAACAATAATGAACGTGTGTGGAATATGATCCAAAAGGTTGGACAAATTGAAGATTTTCT
  ATACCAATTAGAGGACAGTTTCTTAAAAACTAAAAAACTGAGAACTGCTCGAAGGCAAAAAACAAAAATG
  AAGCGACTTCAAACTGTTCAGCAAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205462 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDAGSSRSLENAVNRIYHDQLVPKINTSKKMSTLAHPPNILEMSQEIKKNCGGKQVEITLERTKMTKGIK
  EKQSNDLEKAAFKRKAEGEGKPTRKKQAKITELDNQLITMPLPHIPLKNIMDVEMKLVYIDEMGVRYEFV
  ESFMSTGSQPTCQAAEIVDPLSVHNFSFLPQIDKWLQVALKDASSCYRQKKYALAAGQFRTALELCSKGA
  VLGEPFDAPAEDIASVASFIETKLVTCYLRMRKPDLALNHAHRSIVLNPAYFRNHLRQATVFRCLERYSE
  AARSAMIADYMFWLGGGREESISKLIKLYWQAMIEEAITRAESFSVMYTPFATKIRADKIEKVKDAFTKT
  HPAYAEYMYTDLQALHMLPQTVDWSSFPPQQYLLTLGFKNKDDGKFLEKISSRKLPIFTEHKTPFGLTRE
  DTVRQMETMGKRILPILDFIRSTQLNGSFPASSGVMEKLQYASLLSQLQRVKEQSQVINQAMAELATIPY
  LQDISQQEAELLQSLMADAMDTLEGRRNNNERVWNMIQKVGQIEDFLYQLEDSFLKTKKLRTARRQKTKM
  KRLQTVQQR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031955
• ORF Size: 1707 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031955.6
• RefSeq Size: 2122 bp
• RefSeq ORF: 1710 bp
• Locus ID: 83893
• UniProt ID: Q9BXB7
• Cytogenetics: 3q26.31
• MW: 65.2 kDa
• Gene Summary: This gene encodes a testis-specific protein that belongs to the tetratricopeptide repeat-like superfamily. The encoded protein localizes to the Golgi apparatus and may play a role in spermatogenesis. [provided by RefSeq, May 2010]