ATP6V1B2 (NM_001693) Human Tagged ORF Clone

CAT#: RC205447

ATP6V1B2 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2 (ATP6V1B2)

Product Images

Circular map for RC205447

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ATP6V1B2 (Cat# RC205447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B2 (Cat# TA505244). Positive lysates LY419793 (100ug) and LC419793 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY419793) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205447 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ATP6V1B2 protein (Cat# TP305447). The protein was produced from HEK293T cells transfected with ATP6V1B2 cDNA clone (Cat# RC205447) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP6V1B2
• Synonyms: ATP6B1B2; ATP6B2; DOOD; HO57; VATB; Vma2; VPP3; ZLS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205447 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCGGGCGATGCGGGGGATTGTCAACGGGGCCGCACCCGAGCTACCCGTGCCCACCGGTGGGC
  CGGCGGTGGGAGCTCAGGAGCAGGCGCTGGCAGTCAGTCGGAACTACCTCTCCCAGCCTCGCCTCACATA
  CAAGACAGTATCTGGAGTCAATGGTCCACTAGTGATCTTAGATCATGTTAAGTTTCCCAGGTATGCTGAA
  ATTGTCCATTTGACCTTACCGGATGGCACAAAGAGAAGTGGGCAAGTTCTGGAAGTTAGTGGTTCCAAGG
  CAGTAGTTCAGGTATTTGAAGGGACTTCAGGTATAGATGCTAAGAAAACGTCCTGTGAGTTTACTGGGGA
  TATTCTCCGAACACCGGTGTCTGAGGATATGCTTGGTCGGGTATTCAATGGATCGGGAAAACCCATTGAC
  AGAGGTCCTGTTGTACTGGCCGAAGACTTCCTTGATATCATGGGTCAGCCAATCAACCCTCAATGTCGAA
  TCTACCCAGAGGAAATGATTCGGACTGGCATTTCGGCCATCGATGGGATGAACAGTATTGCTAGGGGGCA
  GAAAATTCCTATCTTCTCTGCTGCTGGGCTACCACACAATGAGATTGCAGCTCAGATCTGTCGCCAGGCT
  GGTTTGGTAAAGAAATCCAAAGATGTAGTAGACTACAGTGAGGAAAATTTTGCAATTGTATTTGCTGCTA
  TGGGTGTAAACATGGAAACTGCCCGGTTCTTCAAATCTGACTTTGAAGAAAATGGCTCAATGGACAATGT
  CTGCCTCTTTTTGAACTTGGCTAATGACCCAACCATTGAGCGAATTATCACTCCTCGCCTGGCTCTAACC
  ACAGCTGAATTTCTGGCGTACCAATGTGAGAAACATGTATTGGTTATTCTAACAGACATGAGTTCTTATG
  CTGAAGCACTTCGAGAGGTTTCAGCAGCCAGGGAAGAGGTACCTGGTCGACGAGGTTTTCCAGGTTACAT
  GTATACAGATTTAGCCACGATATATGAACGCGCTGGGCGAGTGGGAGGGAGAAACGGCTCGATTACTCAA
  ATCCCTATTCTAACCATGCCTAATGATGATATCACTCACCCCATCCCAGACTTGACTGGCTACATTACAG
  AGGGGCAGATCTATGTGGACAGACAGCTGCACAACAGACAGATTTATCCACCTATCAATGTGCTGCCCTC
  ACTATCACGGTTAATGAAGTCTGCTATTGGAGAAGGGATGACCAGGAAGGATCATGCCGATGTATCTAAC
  CAGCTATATGCGTGCTATGCTATTGGAAAGGATGTGCAAGCCATGAAAGCTGTCGTTGGAGAAGAAGCCC
  TTACCTCAGATGATCTTCTCTACTTGGAATTTCTGCAGAAGTTTGAGAGGAACTTCATTGCTCAGGGTCC
  TTACGAAAATCGCACTGTCTTTGAGACTTTGGACATTGGCTGGCAGCTACTCCGAATCTTCCCCAAAGAA
  ATGCTGAAGAGAATCCCTCAGAGCACCCTCAGCGAATTTTACCCTCGAGACTCTGCAAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALRAMRGIVNGAAPELPVPTGGPAVGAQEQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAE
  IVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPID
  RGPVVLAEDFLDIMGQPINPQCRIYPEEMIRTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQA
  GLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALT
  TAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVGGRNGSITQ
  IPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSN
  QLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKE
  MLKRIPQSTLSEFYPRDSAKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001693
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3054 bp
• RefSeq ORF: 1536 bp
• Locus ID: 526
• UniProt ID: P21281
• Cytogenetics: 8p21.3
• Domains: ATP-synt_ab, ATP-synt_ab_C, ATP-synt_ab_N
• Protein Families: Druggable Genome
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• MW: 56.4 kDa
• Gene Summary: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]