ACTRT1 (NM_138289) Human Tagged ORF Clone

CAT#: RC205402

ACTRT1 (Myc-DDK-tagged)-Human actin-related protein T1 (ACTRT1)

Product Images

Circular map for RC205402

• 

  Western blot validation of overexpression lysate (Cat# LY403347) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205402 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACTRT1
• Synonyms: AIP1; ARIP1; ARPT1; HSD27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205402 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAATCCACATGCATTAGATGTTCCTGCTGTAATTTTTGACAATGGTTCAGGACTCTGCAAAGCAG
  GCCTGTCTGGAGAGATTGGACCCCGCCATGTCATCAGCTCCGTCTTGGGACATTGTAAATTCAATGTGCC
  TTTAGCAAGACTTAATCAGAAGTACTTCGTGGGGCAAGAAGCCCTGTACAAGTATGAGGCCCTACATTTG
  CACTACCCCATTGAGCGTGGACTGGTAACAGGATGGGATGACATGGAGAAACTCTGGAAACATCTCTTTG
  AGCGGGAGCTTGGAGTAAAACCCAGCCAACAGCCTGTACTTATGACCGAGCCCTCTTTGAATCCTAGGGA
  AATTCGAGAAAAGCTAGCAGAAATGATGTTTGAGACCTTCAGTGTGCCTGGTTTCTACCTGTCTAATCAT
  GCGGTGGCAGCGCTCTATGCCTCTGCCTGTGTCACAGGCCTGGTGGTGGACAGTGGAGATGGGGTCACTT
  GCACTGTCCCCATCTTTGAGGGTTACTCCCTGCCTCACGCAGTCACCAAACTCTGTATGGCAGGGAGGGA
  CATCACAGAGCACCTCACCCGGCTCCTCTTTGCTAGCGGGTTTAACTTCCCTTGCATACTCAACAAGGCC
  GTGGTAAATAACATCAAAGAGAAGTTGTGCTACATCGCCTTGGAGCCAGAGAAAGAGCTACGCAAGAGCC
  GGGGAGAGGTCCTGGGAGCATACAGACTGCCAGATGGACATGTCATCCACTTTGGGGATGAGCTGTACCA
  AGTGCCCGAGGTTCTTTTTGCACCTGACCAGCTGGGCATCCACAGCCCAGGACTCTCAAAAATGGTCTCC
  AGCAGCATCATGAAGTGTGACACTGACATCCAGAATAAACTTTATGCAGACATTGTACTCTCCGGGGGCA
  CCACTCTCCTCCCTGGGCTGGAGGAAAGGCTCATGAAGGAAGTGGAACAGCTGGCTTCCAAAGGTACTCC
  CATCAAGATCACAGCTTCTCCTGATAGATGCTTCTCTGCATGGATTGGTGCATCCATCATGACCTCTATG
  AGCAGTTTCAAGCAGATGTGGGTCACCTCGGCAGACTTCAAGGAGTATGGGACATCTGTGGTTCAAAGAA
  GGTGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205402 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFNPHALDVPAVIFDNGSGLCKAGLSGEIGPRHVISSVLGHCKFNVPLARLNQKYFVGQEALYKYEALHL
  HYPIERGLVTGWDDMEKLWKHLFERELGVKPSQQPVLMTEPSLNPREIREKLAEMMFETFSVPGFYLSNH
  AVAALYASACVTGLVVDSGDGVTCTVPIFEGYSLPHAVTKLCMAGRDITEHLTRLLFASGFNFPCILNKA
  VVNNIKEKLCYIALEPEKELRKSRGEVLGAYRLPDGHVIHFGDELYQVPEVLFAPDQLGIHSPGLSKMVS
  SSIMKCDTDIQNKLYADIVLSGGTTLLPGLEERLMKEVEQLASKGTPIKITASPDRCFSAWIGASIMTSM
  SSFKQMWVTSADFKEYGTSVVQRRCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138289
• ORF Size: 1128 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138289.4
• RefSeq Size: 1452 bp
• RefSeq ORF: 1131 bp
• Locus ID: 139741
• UniProt ID: Q8TDG2
• Cytogenetics: Xq25
• MW: 41.7 kDa
• Gene Summary: This gene encodes a protein related to the cytoskeletal protein beta-actin. This protein is a major component of the calyx in the perinuclear theca of mammalian sperm heads, and it therefore likely functions in spermatid formation. This gene is intronless and is similar to a related gene located on chromosome 1. A related pseudogene has also been identified approximately 75 kb downstream of this gene on chromosome X. [provided by RefSeq, May 2010]