Protein phosphatase inhibitor 1 (PPP1R1A) (NM_006741) Human Tagged ORF Clone

CAT#: RC205263

PPP1R1A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 1A (PPP1R1A)

Product Images

Circular map for RC205263

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  Western blot validation of overexpression lysate (Cat# LY402013) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205263 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PPP1R1A protein (Cat# TP305263). The protein was produced from HEK293T cells transfected with PPP1R1A cDNA clone (Cat# RC205263) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Protein phosphatase inhibitor 1
• Synonyms: I1; IPP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205263 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAAGACAACAGCCCCCAAAAGATCCAGTTCACGGTCCCGCTGCTGGAGCCGCACCTTGACCCCG
  AGGCGGCGGAGCAGATTCGGAGGCGCCGCCCCACCCCTGCCACCCTCGTGCTGACCAGTGACCAGTCATC
  CCCAGAGATAGATGAAGACCGGATCCCCAACCCACATCTCAAGTCCACTTTGGCAATGTCTCCACGGCAA
  CGGAAGAAGATGACAAGGATCACACCCACAATGAAAGAGCTCCAGATGATGGTTGAACATCACCTGGGGC
  AACAGCAGCAAGGAGAGGAACCTGAGGGGGCCGCTGAGAGCACAGGAACCCAGGAGTCCCGCCCACCTGG
  GATCCCAGACACAGAAGTGGAGTCAAGGCTGGGCACCTCTGGGACAGCAAAAAAAACTGCAGAATGCATC
  CCTAAAACTCACGAAAGAGGCAGTAAGGAACCCAGCACAAAAGAACCCTCAACCCATATACCACCACTGG
  ATTCCAAGGGAGCCAACTCGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205263 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQDNSPQKIQFTVPLLEPHLDPEAAEQIRRRRPTPATLVLTSDQSSPEIDEDRIPNPHLKSTLAMSPRQ
  RKKMTRITPTMKELQMMVEHHLGQQQQGEEPEGAAESTGTQESRPPGIPDTEVESRLGTSGTAKKTAECI
  PKTHERGSKEPSTKEPSTHIPPLDSKGANSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006741
• ORF Size: 513 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006741.2, NP_006732.2
• RefSeq Size: 1849 bp
• RefSeq ORF: 516 bp
• Locus ID: 5502
• UniProt ID: Q13522
• Cytogenetics: 12q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Long-term potentiation
• MW: 18.9 kDa
• Gene Summary: Inhibitor of protein-phosphatase 1. This protein may be important in hormonal control of glycogen metabolism. Hormones that elevate intracellular cAMP increase I-1 activity in many tissues. I-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. Following a rise in intracellular calcium, I-1 is inactivated by calcineurin (or PP2B). Does not inhibit type-2 phosphatases.[UniProtKB/Swiss-Prot Function]