Dynein intermediate chain 1 (DNAI1) (NM_012144) Human Tagged ORF Clone

CAT#: RC205242

DNAI1 (Myc-DDK-tagged)-Human dynein, axonemal, intermediate chain 1 (DNAI1)

Product Images

Circular map for RC205242

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  Western blot validation of overexpression lysate (Cat# LY402158) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205242 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DNAI1 protein (Cat# TP305242). The protein was produced from HEK293T cells transfected with DNAI1 cDNA clone (Cat# RC205242) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dynein intermediate chain 1
• Synonyms: CILD1; DIC1; ICS1; PCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205242 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCCTGCTTCTGCGAAGTCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCATAGGCAGAGGAA
  CCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAGATGAATGGGCCCAATCCAAAGC
  CACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATGCGGAGTTAAAGGAGGAGTTCACTCGGATTTTG
  ACAGCCAACAACCCACACGCACCCCAGAACATTGTCAGGTACAGCTTCAAAGAAGGCACATATAAGCCTA
  TTGGCTTTGTGAACCAACTGGCAGTTCACTACACCCAGGTTGGGAACCTGATCCCCAAAGACTCAGATGA
  AGGACGGCGGCAGCATTACCGCGATGAATTAGTGGCAGGTTCTCAGGAGTCTGTCAAGGTGATTTCAGAA
  ACAGGAAACCTCGAAGAAGACGAAGAGCCCAAGGAGTTAGAAACTGAGCCTGGGAGTCAAACAGATGTGC
  CTGCAGCTGGGGCAGCTGAAAAAGTGACTGAAGAAGAATTGATGACTCCTAAGCAGCCCAAGGAGAGAAA
  GCTCACTAACCAGTTCAACTTCAGTGAGAGGGCCTCACAGACCTGCAACAACCCTGTCCGGGATCGAGAA
  TGCCAGACGGAGCCTCCTCCCAGGACAAACTTTTCAGCCACAGCCAATCAGTGGGAGATCTATGATGCCT
  ATGTAGAGGAACTTGAGAAGCAGGAAAAGACCAAAGAGAAGGAGAAGGCAAAGACCCCAGTGGCTAAAAA
  ATCAGGGAAGATGGCCATGAGGAAGCTGACATCTATGGAGTCTCAGACTGATGATCTCATCAAATTGTCC
  CAAGCTGCTAAGATCATGGAGCGGATGGTCAACCAGAATACATATGATGACATTGCTCAAGATTTTAAGT
  ACTATGACGATGCTGCTGATGAATACCGGGACCAGGTGGGTACCCTGCTGCCGCTCTGGAAGTTCCAAAA
  TGACAAAGCCAAGCGCCTGTCCGTCACTGCCCTCTGCTGGAATCCAAAGTACAGGGATCTGTTTGCAGTG
  GGATATGGCTCTTATGACTTCATGAAGCAGAGCCGGGGCATGCTGCTGCTCTACAGCCTGAAGAACCCCA
  GCTTCCCTGAGTACATGTTCAGCAGCAACAGCGGCGTCATGTGTCTCGACATCCACGTGGACCACCCCTA
  CCTGGTGGCAGTAGGCCACTATGACGGCAACGTGGCCATTTACAACCTCAAGAAGCCCCACTCCCAGCCC
  TCCTTCTGCAGCTCAGCCAAGTCTGGCAAGCACTCAGACCCTGTGTGGCAGGTCAAGTGGCAGAAGGATG
  ACATGGACCAAAACCTTAACTTCTTCTCTGTGTCATCTGACGGCAGGATTGTGTCTTGGACTCTCGTGAA
  GAGAAAGCTGGTTCACATAGATGTCATCAAGCTGAAGGTGGAAGGCAGCACCACGGAAGTTCCTGAGGGG
  TTGCAGCTGCACCAAGTGGGTTGTGGCACTGCCTTTGACTTCCACAAAGAGATTGACTACATGTTCCTAG
  TGGGCACAGAGGAGGGAAAAATCTACAAGTGCTCTAAATCCTACTCCAGCCAATTCCTCGACACCTATGA
  CGCCCACAACATGTCAGTGGACACTGTGTCCTGGAACCCATACCACACCAAGGTCTTCATGTCCTGCAGC
  TCCGACTGGACAGTGAAGATCTGGGACCACACCATCAAGACCCCGATGTTCATCTATGACCTGAACTCAG
  CCGTGGGTGATGTGGCCTGGGCGCCATACTCTTCTACTGTGTTCGCAGCAGTCACCACAGATGGGAAGGC
  CCACATATTTGACTTAGCCATCAACAAGTATGAGGCCATCTGCAACCAGCCTGTGGCGGCCAAAAAGAAC
  AGGCTCACCCACGTGCAGTTCAATCTCATCCACCCCATCATCATTGTGGGCGATGACCGTGGGCACATCA
  TCAGCCTCAAGCTCTCACCCAATTTGCGCAAGATGCCAAAGGAAAAGAAGGGGCAGGAGGTGCAGAAGGG
  TCCAGCTGTGGAGATTGCGAAACTGGACAAACTGCTGAACCTGGTGAGGGAAGTGAAAATCAAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205242 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIPASAKSPHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRIL
  TANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQVGNLIPKDSDEGRRQHYRDELVAGSQESVKVISE
  TGNLEEDEEPKELETEPGSQTDVPAAGAAEKVTEEELMTPKQPKERKLTNQFNFSERASQTCNNPVRDRE
  CQTEPPPRTNFSATANQWEIYDAYVEELEKQEKTKEKEKAKTPVAKKSGKMAMRKLTSMESQTDDLIKLS
  QAAKIMERMVNQNTYDDIAQDFKYYDDAADEYRDQVGTLLPLWKFQNDKAKRLSVTALCWNPKYRDLFAV
  GYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAIYNLKKPHSQP
  SFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLKVEGSTTEVPEG
  LQLHQVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSWNPYHTKVFMSCS
  SDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKYEAICNQPVAAKKN
  RLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLDKLLNLVREVKIKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012144
• ORF Size: 2097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012144.4
• RefSeq Size: 2593 bp
• RefSeq ORF: 2100 bp
• Locus ID: 27019
• UniProt ID: Q9UI46
• Cytogenetics: 9p13.3
• Domains: WD40
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• MW: 79.3 kDa
• Gene Summary: This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]