CCDC87 (NM_018219) Human Tagged ORF Clone

CAT#: RC205226

CCDC87 (Myc-DDK-tagged)-Human coiled-coil domain containing 87 (CCDC87)

Product Images

Circular map for RC205226

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  Western blot validation of overexpression lysate (Cat# LY413200) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205226 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCDC87 protein (Cat# TP305226). The protein was produced from HEK293T cells transfected with CCDC87 cDNA clone (Cat# RC205226) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC87
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205226 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGCCCCCGAAGCCCGAGCCTGAGCTCCAGCGGTTTTACCACCGGCTGCTGCGTCCGCTGTCGC
  TCTTCCCCACTAGGACGACGTCCCCAGAGCCTCAGAAGCGCCCCCCGCAGGAGGGCCGGATTCTGCAGTC
  CTTCCCTCTGGCGAAGCTGACGGTGGCGTCGCTGTGCAGCCAGGTGGCCAAGCTGCTGGCCGGCAGCGGG
  ATAGCAGCGGGAGTGCCTCCTGAGGCCCGACTACGTCTCATCAAGGTCATCCTGGACGAGCTGAAGTGCA
  GCTGGCGGGAGCCGCCCGCCGAACTTAGTCTGAGCCACAAAAACAACCAGAAGCTGCGGAAGCGGCTCGA
  GGCCTACGTGCTGCTGAGCAGCGAGCAGCTCTTCTTGCGCTACCTGCACCTGCTGGTGACCATGTCGACT
  CCCAGGGGGGTCTTCACTGAATCAGCCACCCTCACCCGGTTGGCCGCCAGCCTCGCCAGGGACTGCACAC
  TCTTCCTTACTAGTCCCAACGTCTACCGTGGCCTGCTTGCCGACTTCCAGGCCCTGCTGAGGGCAGAGCA
  GGCCTCTGGGGATGTGGACAAGCTGCACCCTGTCTGCCCCGCTGGGACGTTCAAGCTGTGCCCTATCCCC
  TGGCCTCACAGCACTGGCTTAGCCCAAGTGCAGTGCTCTAACCTCAACCTGAACTACCTCATCCAACTCA
  GCCGTCCACCAGAGTTTCTCAATGAGCCAGGAAGGATGGATCCAGTGAAGGAATTGAAGTCCATCCCTCG
  GTTGAAGAGGAAAAAGCCTTTCCACTGGCTGCCCTCCATAGGAAAGAAGAGAGAAATCGACATCAGTTCC
  TCACAGATGGTGTCGCTGCCCAGCTATCCTGTGGCCCCCACCAGCAGGGCTTCCCCCTCGCCTTTCTGCC
  CTGAGCTCCGGAGAGGCCAATCCATGCCCTCCCTGCGTGAGGGCTGGAGGCTGGCAGATGAGTTGGGCCT
  TCCTCCACTCCCATCTCGCCCCTTAACCCCGCTGGTCTTGGCTACAGAGAGCAAACCAGAGCTGACTGGG
  CTCATCGTGGCTGAGGATCTGAAGCAGTTGATAAAGAAGATGAAGTTGGAGGGGACTCGCTACCCACCAC
  TGGACTCAGGCCTGCCTCCTCTCCTGGGGGTTGTGACCCGTCACCCAGCTGCAGGGCATCGCCTGGAGGA
  GCTGGAGAAGATGTTGAGGAACCTCCAGGAGGAAGAAGCCTCTGGGCAGTGGGACCCCCAGCCCCCCAAA
  TCCTTTCCACTTCACCCACAGCCAGTGACCATTACTTTGAAGCTTAGAAATGAGGTCGTGGTCCAGGCGG
  CTGCCGTACGGGTCTCTGATAGAAACTTCTTAGACTCTTTCCACATTGAGGGGGCCGGAGCCCTGTATAA
  CCATCTGGCTGGTGAACTGGATCCCAAAGCCATTGAAAAAATGGATATTGATAACTTTGTTGGCAGTACT
  ACCAGGGAGGTCTACAAGGAGTTGATGAGCCATGTCTCTTCTGACCACTTACATTTTGATCAAGGGCCCC
  TAGTTGAGCCTGCAGCAGATAAAGACTGGTCGACCTTCCTGTCCTCAGCCTTTCTACGTCAAGAAAAACA
  GCCTCAAATCATCAACCCTGAGCTGGTTGGACTTTACTCCCAGAGAGCAAACACTTTACAGTCCAATACT
  AAGAAGATGCCCTCCCTCCCATCACTCCAAGCTACCAAAAGCTGGGAGAAGTGGTCAAACAAGGCCTCCT
  TGATGAACTCATGGAAAACCACCTTGTCTGTGGATGACTACTTCAAGTACCTCACCAACCATGAAACAGA
  TTTCCTTCATGTCATCTTTCAAATGCATGAAGAAGAGGTTCCTGTGGAGATTGTGGCCCCTGCCAGAGAG
  TCCCTAGAGATTCAGCACCCTCCCCCATTGCTAGAAGATGAAGAACCAGACTTTGTGCCAGGAGAGTGGG
  ATTGGAACACTGTGCTAGAGCACAGGCTAGGAGCTGGGAAGACACCCCACCTGGGAGAACCCCACAAAAT
  TCTGAGCCTGCAGAAGCATCTGGAACAACTGTGGTCTGTGCTTGAGGTCCCTGACAAGGACCAGGTGGAC
  ATGACCATTAAATATAGCTCCAAAGCCCGCCTGAGGCAGCTGCCTTCATTGGTGAATGCCTGGGAGCGGG
  CCCTGAAGCCCATTCAGCTGCGGGAGGCATTGCTGGCGAGACTAGAGTGGTTTGAGGGACAAGCTTCCAA
  TCCCAACCGCTTCTTCAAAAAGACCAACTTGAGCTCCAGTCACTTCCTGGAGGAGAATCAGGTCCGAAGC
  CATCTCCACAGGAAGCTCAACTTAATGGAGTCTTCTTTGGTTTCCCTCCTAGAGGAGATAGAGTTAATCT
  TTGGCGAGCCAGTGATCTTCAAGGGGCGGCCCTACCTGGACAAGATGAAGAGTGACAAAGTGGAGATGCT
  CTATTGGCTGCAACAGCAGCGGCGGGTTCGCCACCTGGTCTCGGCCCTGAAGGATCCCCACCAGTCAACC
  CTGTTCAGGAGCTCAGCAGCCAGCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205226 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMEPPKPEPELQRFYHRLLRPLSLFPTRTTSPEPQKRPPQEGRILQSFPLAKLTVASLCSQVAKLLAGSG
  IAAGVPPEARLRLIKVILDELKCSWREPPAELSLSHKNNQKLRKRLEAYVLLSSEQLFLRYLHLLVTMST
  PRGVFTESATLTRLAASLARDCTLFLTSPNVYRGLLADFQALLRAEQASGDVDKLHPVCPAGTFKLCPIP
  WPHSTGLAQVQCSNLNLNYLIQLSRPPEFLNEPGRMDPVKELKSIPRLKRKKPFHWLPSIGKKREIDISS
  SQMVSLPSYPVAPTSRASPSPFCPELRRGQSMPSLREGWRLADELGLPPLPSRPLTPLVLATESKPELTG
  LIVAEDLKQLIKKMKLEGTRYPPLDSGLPPLLGVVTRHPAAGHRLEELEKMLRNLQEEEASGQWDPQPPK
  SFPLHPQPVTITLKLRNEVVVQAAAVRVSDRNFLDSFHIEGAGALYNHLAGELDPKAIEKMDIDNFVGST
  TREVYKELMSHVSSDHLHFDQGPLVEPAADKDWSTFLSSAFLRQEKQPQIINPELVGLYSQRANTLQSNT
  KKMPSLPSLQATKSWEKWSNKASLMNSWKTTLSVDDYFKYLTNHETDFLHVIFQMHEEEVPVEIVAPARE
  SLEIQHPPPLLEDEEPDFVPGEWDWNTVLEHRLGAGKTPHLGEPHKILSLQKHLEQLWSVLEVPDKDQVD
  MTIKYSSKARLRQLPSLVNAWERALKPIQLREALLARLEWFEGQASNPNRFFKKTNLSSSHFLEENQVRS
  HLHRKLNLMESSLVSLLEEIELIFGEPVIFKGRPYLDKMKSDKVEMLYWLQQQRRVRHLVSALKDPHQST
  LFRSSAASL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018219
• ORF Size: 2547 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018219.3
• RefSeq Size: 2915 bp
• RefSeq ORF: 2550 bp
• Locus ID: 55231
• UniProt ID: Q9NVE4
• Cytogenetics: 11q13.2
• MW: 96.4 kDa
• Gene Summary: Plays a role in spermatogenesis, where it is important for normal sperm head morphology. Also required for the acrosome reaction and thus normal male fertility.[UniProtKB/Swiss-Prot Function]