LRRC36 (NM_018296) Human Tagged ORF Clone

CAT#: RC205223

LRRC36 (Myc-DDK-tagged)-Human leucine rich repeat containing 36 (LRRC36), transcript variant 1

Product Images

Circular map for RC205223

• 

  Western blot validation of overexpression lysate (Cat# LY413144) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205223 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC36
• Synonyms: RORBP70; XLHSRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205223 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCAATGGGAGCTGGACGAGGAAGGCATTCGCCGCCTGGGGGCGCTGACGCTGGAGCAGCCGG
  AACTGGTGGAGTCTCTTTCATTGCAGGGATCTTATGCTGGCAAAATCCATTCCATTAGTGATGCCTTCAG
  AAATTTTAAAAATCTCCGATCCTTAGATTTATCAAGGAATTTGATCACTAGCCTTAAGGGAATCCAGTAT
  TTATGTTCACTCCAAGACCTGAATTTATATTATAACAACATTCCTTCATTAGTGGAAGTGTCCCGTCTAC
  AACCGTTACCCTTCCTCAAAGAACTGGATTTGAGACTTAATCCTGTTGTAAGGAAAGATACAGATTATAG
  GCTCTTTGCTGTATATACACTACAAACTCTGGAGAAATTAGATGACCGAACTGTACGTGAAGGTGAGAGA
  AAAGCTGCCAAGCTGCATTTTAGTCAGTTGGGCAACAGTGAAAATTTTCTTTTAGAGGTGGAAAAAAGCT
  CTAGGGAGAAGACAATGAAAAACTGTGTAACAGGTGAGAGCTCTGCATCAAAAGTCAGTGCTAATGTTGA
  CAGCAGGATTGAAATGGACTCAAACAAAGGACTTTTTATTCCCTTCCCCAACCGGGAAATAAAGGATTCC
  CTAAGTACTTCTGCAACTCAGGGCAATGGTACACGTGATCAGAAATTAGACACCTTCCCACTGGGGACAC
  AGACACAGGAAGTAGCAAGAAGGGAGATGCCAAGTGACAATCACCAGGAAGATGAGTTCAGACACTACTC
  GCCTCGTCAGTCCACAGTCCGATCCCCAGAGAAGATGACTAGAGAAGGGTACCAAGTATCTTTTTTGGAC
  AATAAGTCTTCAGGTTCTTCTCCAGAAAAGGAATTGATACCAAAACCTGATACTTTTCATCTTACCCATG
  ATGCCTCATTGAGTAAATGCCTGGATGTGGGTGATTCTAGCCAGATCCATCCCTATCAGTTACCTTCAGA
  TGTTGGTCTGGAAAATTATGACAGTTGTTATTCTCAAACTCTATCCCTGCATGGAAGTCTTGGTAAAAGG
  CCTCAGAGAAGCAAGAACTATCAAGAGTATAGCATAAAGCCTTCAAATGACATAAAGACCACCGCTTCAC
  ATTCCTGTGGAGACTTATTAACTTCTCTGTCAAACCCTGACTCCAGCACTGGAAGGCTTTTGAAGCTTAG
  TTCAGATCTGTATGCCACAACCCATTTCAACAGTGACCCTGCTGTACTTGTCAATGTAGAGCAACAATTA
  TCTACCAGCCTGGATGATTTAACACCAGCACATGGTTCTGTCCCAAACAACGCTGTCCTGGGAAACAGGA
  CAACTCCTCTGCGGACACTGCTGTTGTCTCCTGGGACTTCAGAACACAGAAAGATTTTTACCAAGAGGTC
  ACTAAGCCCATCGAAGAGAGGATTCAAATGGAAGGACAATATCCTTGCCAACCTGAATCTAAAGCATGGT
  TTCCAAGATGCTACAGGCAGCGAGCCTCTCTCTAGTGACCTGGGTAGTTTGCACGGTTTGGCTGGAAACC
  ACAGTCCCCCCATCTCTGCCAGAACCCCCCATGTGGCCACTGTCCTCAGACAGCTCCTGGAGCTTGTGGA
  TAAGCACTGGAATGGCTCCGGCTCCCTCCTCCTCAACAAGAAGTTTCTCGGTCCTGCCCGAGATTTGCTT
  CTGTCTTTGGTAGTCCCGGCTCCTTCTCAGCCGAGGTGTTGCTCACATCCTGAAGACACGATGAAAGCAT
  TCTGCAGGAGGGAGCTTGAACTGAAGGAGGCTGCGCAGCTGGTCCCTAATGACATGGAAAGTTTGAAGCA
  AAAACTGGTCAGAGTGCTGGAGGAAAACCTCATTTTGTCAGAAAAAATTCAACAGTTGGAGGAAGGTGCT
  GCCATCTCAATTGTGAGTGGGCAACAGTCACATACTTATGATGATCTTCTGCACAAAAACCAACAGCTGA
  CCATGCAGGTGGCTTGCCTGAACCAGGAGCTTGCCCAGCTGAAAAAGCTGGAGAAGACAGTTGCCATTCT
  CCATGAAAGTCAGAGATCCCTGGTGGTAACTAATGAGTATCTGCTGCAGCAGCTGAATAAGGAGCCAAAA
  GGTTATTCCGGGAAAGCGCTCCTGCCTCCTGAGAAGGGTCATCATCTGGGGAGATCATCGCCCTTTGGGA
  AAAGCACGTTGTCTTCCTCCTCACCAGTGGCACATGAGACTGGTCAGTATCTAATACAGAGCGTCTTGGA
  TGCTGCCCCAGAGCCTGGCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205223 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEQWELDEEGIRRLGALTLEQPELVESLSLQGSYAGKIHSISDAFRNFKNLRSLDLSRNLITSLKGIQY
  LCSLQDLNLYYNNIPSLVEVSRLQPLPFLKELDLRLNPVVRKDTDYRLFAVYTLQTLEKLDDRTVREGER
  KAAKLHFSQLGNSENFLLEVEKSSREKTMKNCVTGESSASKVSANVDSRIEMDSNKGLFIPFPNREIKDS
  LSTSATQGNGTRDQKLDTFPLGTQTQEVARREMPSDNHQEDEFRHYSPRQSTVRSPEKMTREGYQVSFLD
  NKSSGSSPEKELIPKPDTFHLTHDASLSKCLDVGDSSQIHPYQLPSDVGLENYDSCYSQTLSLHGSLGKR
  PQRSKNYQEYSIKPSNDIKTTASHSCGDLLTSLSNPDSSTGRLLKLSSDLYATTHFNSDPAVLVNVEQQL
  STSLDDLTPAHGSVPNNAVLGNRTTPLRTLLLSPGTSEHRKIFTKRSLSPSKRGFKWKDNILANLNLKHG
  FQDATGSEPLSSDLGSLHGLAGNHSPPISARTPHVATVLRQLLELVDKHWNGSGSLLLNKKFLGPARDLL
  LSLVVPAPSQPRCCSHPEDTMKAFCRRELELKEAAQLVPNDMESLKQKLVRVLEENLILSEKIQQLEEGA
  AISIVSGQQSHTYDDLLHKNQQLTMQVACLNQELAQLKKLEKTVAILHESQRSLVVTNEYLLQQLNKEPK
  GYSGKALLPPEKGHHLGRSSPFGKSTLSSSSPVAHETGQYLIQSVLDAAPEPGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018296
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018296.4, NP_060766.4
• RefSeq Size: 2416 bp
• RefSeq ORF: 2265 bp
• Locus ID: 55282
• UniProt ID: Q1X8D7
• Cytogenetics: 16q22.1
• MW: 83.9 kDa