GPRC5B (NM_016235) Human Tagged ORF Clone

CAT#: RC205201

GPRC5B (Myc-DDK-tagged)-Human G protein-coupled receptor, family C, group 5, member B (GPRC5B)

Product Images

Circular map for RC205201

• 

  Western blot validation of overexpression lysate (Cat# LY402523) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205201 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPRC5B
• Synonyms: RAIG-2; RAIG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205201 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGTGGCATCAGAGAGAAAGATGAGAGCTCACCAGGTGCTCACCTTCCTCCTGCTCTTCGTGATCA
  CCTCGGTGGCCTCTGAAAACGCCAGCACATCCCGAGGCTGTGGGCTGGACCTCCTCCCTCAGTACGTGTC
  CCTGTGCGACCTGGACGCCATCTGGGGCATTGTGGTGGAGGCGGTGGCCGGGGCGGGCGCCCTGATCACA
  CTGCTCCTGATGCTCATCCTCCTGGTGCGGCTGCCCTTCATCAAGGAGAAGGAGAAGAAGAGCCCTGTGG
  GCCTCCACTTTCTGTTCCTCCTGGGGACCCTGGGCCTCTTTGGGCTGACGTTTGCCTTCATCATCCAGGA
  GGACGAGACCATCTGCTCTGTCCGCCGCTTCCTCTGGGGCGTCCTCTTTGCGCTCTGCTTCTCCTGCCTG
  CTGAGCCAGGCATGGCGCGTGCGGAGGCTGGTGCGGCATGGCACGGGCCCCGCGGGCTGGCAGCTGGTGG
  GCCTGGCGCTGTGCCTGATGCTGGTGCAAGTCATCATCGCTGTGGAGTGGCTGGTGCTCACCGTGCTGCG
  TGACACAAGGCCAGCCTGCGCCTACGAGCCCATGGACTTTGTGATGGCCCTCATCTACGACATGGTACTG
  CTTGTGGTCACCCTGGGGCTGGCCCTCTTCACTCTGTGCGGCAAGTTCAAGAGGTGGAAGCTGAACGGGG
  CCTTCCTCCTCATCACAGCCTTCCTCTCTGTGCTCATCTGGGTGGCCTGGATGACCATGTACCTCTTCGG
  CAATGTCAAGCTGCAGCAGGGGGATGCCTGGAACGACCCCACCTTGGCCATCACGCTGGCGGCCAGCGGC
  TGGGTCTTCGTCATCTTCCACGCCATCCCTGAGATCCACTGCACCCTTCTGCCAGCCCTGCAGGAGAACA
  CGCCCAACTACTTCGACACGTCGCAGCCCAGGATGCGGGAGACGGCCTTCGAGGAGGACGTGCAGCTGCC
  GCGGGCCTATATGGAGAACAAGGCCTTCTCCATGGATGAACACAATGCAGCTCTCCGAACAGCAGGATTT
  CCCAACGGCAGCTTGGGAAAAAGACCCAGTGGCAGCTTGGGGAAAAGACCCAGCGCTCCGTTTAGAAGCA
  ACGTGTATCAGCCAACTGAGATGGCCGTCGTGCTCAACGGTGGGACCATCCCAACTGCTCCGCCAAGTCA
  CACAGGAAGACACCTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205201 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFVASERKMRAHQVLTFLLLFVITSVASENASTSRGCGLDLLPQYVSLCDLDAIWGIVVEAVAGAGALIT
  LLLMLILLVRLPFIKEKEKKSPVGLHFLFLLGTLGLFGLTFAFIIQEDETICSVRRFLWGVLFALCFSCL
  LSQAWRVRRLVRHGTGPAGWQLVGLALCLMLVQVIIAVEWLVLTVLRDTRPACAYEPMDFVMALIYDMVL
  LVVTLGLALFTLCGKFKRWKLNGAFLLITAFLSVLIWVAWMTMYLFGNVKLQQGDAWNDPTLAITLAASG
  WVFVIFHAIPEIHCTLLPALQENTPNYFDTSQPRMRETAFEEDVQLPRAYMENKAFSMDEHNAALRTAGF
  PNGSLGKRPSGSLGKRPSAPFRSNVYQPTEMAVVLNGGTIPTAPPSHTGRHLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016235
• ORF Size: 1209 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016235.3
• RefSeq Size: 2870 bp
• RefSeq ORF: 1212 bp
• Locus ID: 51704
• UniProt ID: Q9NZH0
• Cytogenetics: 16p12.3
• Domains: 7tm_3
• Protein Families: Druggable Genome
• MW: 44.8 kDa
• Gene Summary: This gene encodes a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The encoded protein may modulate insulin secretion and increased protein expression is associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]