TSEN2 (NM_025265) Human Tagged ORF Clone

CAT#: RC205156

TSEN2 (Myc-DDK-tagged)-Human tRNA splicing endonuclease 2 homolog (S. cerevisiae) (TSEN2), transcript variant 1

Product Images

Circular map for RC205156

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  Western blot validation of overexpression lysate (Cat# LY428856) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226917 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TSEN2 protein (Cat# TP305156). The protein was produced from HEK293T cells transfected with TSEN2 cDNA clone (Cat# RC205156) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TSEN2
• Synonyms: PCH2B; SEN2; SEN2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205156 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAGCAGTTTTCCATGCCCCAAAGAGGAAAAGAAGAGTGTATGAGACTTACGAGTCTCCATTGC
  CAATCCCTTTTGGTCAGGACCATGGTCCTCTGAAAGAATTCAAGATATTCCGTGCTGAAATGATTAACAA
  CAATGTGATTGTGAGGAATGCGGAGGACATTGAGCAGCTCTATGGGAAAGGTTATTTTGGAAAAGGTATT
  CTTTCAAGAAGCCGTCCAAGCTTCACAATTTCAGATCCTAAACTGGTTGCTAAATGGAAAGATATGAAGA
  CAAACATGCCTATCATCACATCAAAGAGGTATCAGCATAGTGTTGAGTGGGCAGCAGAGCTGATGCGTAG
  ACAGGGGCAGGATGAGAGTACAGTGCGCAGAATCCTCAAGGATTACACGAAACCGCTTGAGCATCCTCCT
  GTGAAAAGGAATGAAGAGGCTCAAGTGCATGACAAGCTTAACTCTGGAATGGTTTCCAACATGGAAGGCA
  CAGCAGGGGGAGAGAGACCTTCTGTGGTAAACGGGGACTCTGGAAAGTCAGGTGGTGTGGGTGATCCCCG
  TGAGCCATTAGGCTGCCTGCAGGAGGGCTCTGGCTGCCACCCAACAACAGAGAGCTTTGAGAAAAGCGTG
  CGAGAGGATGCCTCACCTCTGCCCCATGTCTGTTGCTGCAAACAAGATGCTCTCATCCTCCAGCGTGGCC
  TTCATCATGAAGACGGCAGCCAGCACATCGGCCTCCTGCATCCTGGGGACAGAGGGCCTGACCATGAGTA
  CGTGCTGGTCGAGGAAGCGGAGTGTGCCATGAGCGAGAGGGAGGCTGCCCCAAATGAGGAATTGGTGCAA
  AGAAACAGGTTAATATGCAGAAGAAATCCATATAGGATCTTTGAGTATTTGCAACTCAGCCTAGAAGAGG
  CCTTTTTCTTGGTCTATGCTCTGGGATGTTTAAGTATTTACTATGAGAAGGAGCCTTTAACGATAGTGAA
  GCTCTGGAAAGCTTTCACTGTAGTTCAGCCCACGTTCAGAACCACCTACATGGCCTACCATTACTTTCGA
  AGCAAGGGCTGGGTGCCCAAAGTGGGACTCAAGTACGGGACAGATTTACTGCTATATCGGAAAGGCCCTC
  CATTTTACCATGCAAGTTATTCTGTCATTATCGAGCTAGTTGATGACCATTTTGAAGGCTCTCTCCGCAG
  GCCTCTCAGTTGGAAGTCCCTGGCTGCCTTGAGCAGAGTTTCCGTTAATGTCTCTAAGGAACTTATGCTG
  TGCTATTTGATTAAACCCTCTACTATGACTGACAAGGAAATGGAGTCACCAGAATGTATGAAAAGGATTA
  AAGTTCAGGAGGTGATTCTGAGTCGATGGGTTTCTTCACGAGAGAGGAGTGACCAAGACGATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205156 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEAVFHAPKRKRRVYETYESPLPIPFGQDHGPLKEFKIFRAEMINNNVIVRNAEDIEQLYGKGYFGKGI
  LSRSRPSFTISDPKLVAKWKDMKTNMPIITSKRYQHSVEWAAELMRRQGQDESTVRRILKDYTKPLEHPP
  VKRNEEAQVHDKLNSGMVSNMEGTAGGERPSVVNGDSGKSGGVGDPREPLGCLQEGSGCHPTTESFEKSV
  REDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQ
  RNRLICRRNPYRIFEYLQLSLEEAFFLVYALGCLSIYYEKEPLTIVKLWKAFTVVQPTFRTTYMAYHYFR
  SKGWVPKVGLKYGTDLLLYRKGPPFYHASYSVIIELVDDHFEGSLRRPLSWKSLAALSRVSVNVSKELML
  CYLIKPSTMTDKEMESPECMKRIKVQEVILSRWVSSRERSDQDDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025265
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025265.4
• RefSeq Size: 2402 bp
• RefSeq ORF: 1398 bp
• Locus ID: 80746
• UniProt ID: Q8NCE0
• Cytogenetics: 3p25.2
• Domains: tRNA_int_endo
• MW: 53.2 kDa
• Gene Summary: This gene encodes one of the subunits of the tRNA splicing endonuclease. This endonuclease catalyzes the first step in RNA splicing which is the removal of introns. Mutations in this gene have been associated with pontocerebellar hypoplasia type 2. A pseudogene has been identified on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2009]