SCAMP5 (NM_138967) Human Tagged ORF Clone

CAT#: RC205149

SCAMP5 (Myc-DDK-tagged)-Human secretory carrier membrane protein 5 (SCAMP5), transcript variant 3

Product Images

Circular map for RC205149

• 

  Western blot validation of overexpression lysate (Cat# LY408443) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205149 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCAMP5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205149 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGAAAGTGAACAACTTCCCACCATTGCCCAAATTCATCCCGCTGAAGCCATGTTTCTACCAAG
  ACTTCGAGGCAGATATTCCTCCCCAGCATGTCAGCATGACCAAGCGCCTCTACTACCTCTGGATGTTGAA
  CAGCGTCACGCTGGCCGTGAACCTGGTGGGCTGTCTCGCGTGGCTGATCGGAGGCGGGGGAGCCACCAAC
  TTTGGCCTCGCCTTTCTCTGGCTCATCCTCTTCACACCCTGCTCCTACGTCTGCTGGTTTCGGCCCATTT
  ACAAGGCCTTCAAGACTGACAGCTCCTTCAGTTTCATGGCATTCTTCTTTACCTTCATGGCTCAGTTGGT
  CATCAGCATCATCCAGGCCGTGGGCATCCCAGGCTGGGGCGTCTGCGGCTGGATTGCTACCATCTCCTTC
  TTCGGAACGAACATTGGCTCGGCGGTGGTGATGCTAATTCCCACTGTCATGTTCACAGTGATGGCCGTCT
  TTTCCTTCATCGCCCTCAGCATGGTTCATAAATTTTACCGGGGAAGTGGGGGGAGTTTCAGCAAAGCTCA
  GGAGGAGTGGACCACAGGGGCCTGGAAGAATCCACATGTGCAGCAGGCAGCCCAGAACGCAGCCATGGGG
  GCAGCCCAGGGTGCCATGAATCAGCCTCAGACTCAGTATTCCGCCACCCCCAATTACACGTACTCCAATG
  AGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205149 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEKVNNFPPLPKFIPLKPCFYQDFEADIPPQHVSMTKRLYYLWMLNSVTLAVNLVGCLAWLIGGGGATN
  FGLAFLWLILFTPCSYVCWFRPIYKAFKTDSSFSFMAFFFTFMAQLVISIIQAVGIPGWGVCGWIATISF
  FGTNIGSAVVMLIPTVMFTVMAVFSFIALSMVHKFYRGSGGSFSKAQEEWTTGAWKNPHVQQAAQNAAMG
  AAQGAMNQPQTQYSATPNYTYSNEM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138967
• ORF Size: 705 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138967.4
• RefSeq Size: 3433 bp
• RefSeq ORF: 708 bp
• Locus ID: 192683
• UniProt ID: Q8TAC9
• Cytogenetics: 15q24.2
• Domains: SCAMP
• Protein Families: Secreted Protein, Transmembrane
• MW: 26.1 kDa
• Gene Summary: Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.[UniProtKB/Swiss-Prot Function]