Cofilin 2 (CFL2) (NM_021914) Human Tagged ORF Clone

CAT#: RC205105

CFL2 (Myc-DDK-tagged)-Human cofilin 2 (muscle) (CFL2), transcript variant 1

Product Images

Circular map for RC205105

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  Western blot validation of overexpression lysate (Cat# LY411884) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205105 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CFL2 protein (Cat# TP305105). The protein was produced from HEK293T cells transfected with CFL2 cDNA clone (Cat# RC205105) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cofilin 2
• Synonyms: NEM7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205105 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGGAGTTACAGTGAATGATGAAGTCATCAAAGTTTTTAATGATATGAAAGTAAGGAAATCTT
  CTACACAAGAGGAGATCAAAAAGAGAAAGAAAGCAGTTCTCTTCTGTTTAAGCGATGACAAAAGACAAAT
  AATTGTAGAGGAAGCAAAGCAGATCTTGGTGGGTGACATTGGTGATACTGTAGAGGACCCCTACACATCT
  TTTGTGAAGTTGCTACCTCTGAATGATTGCCGATATGCTTTGTACGATGCCACATACGAAACAAAAGAGT
  CTAAGAAAGAAGACCTAGTATTTATATTCTGGGCTCCTGAAAGTGCACCTTTAAAAAGCAAGATGATTTA
  TGCTAGCTCTAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGTGGCAAGTAAATGGCTTG
  GATGATATTAAGGACCGTTCGACACTTGGAGAGAAATTGGGAGGCAATGTAGTAGTTTCACTTGAAGGAA
  AACCATTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205105 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTS
  FVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGL
  DDIKDRSTLGEKLGGNVVVSLEGKPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021914
• ORF Size: 498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021914.7
• RefSeq Size: 3125 bp
• RefSeq ORF: 501 bp
• Locus ID: 1073
• UniProt ID: Q9Y281
• Cytogenetics: 14q13.1
• Domains: ADF
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton
• MW: 18.7 kDa
• Gene Summary: This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]