TPST2 (NM_001008566) Human Tagged ORF Clone

CAT#: RC204969

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TPST2 (Myc-DDK-tagged)-Human tyrosylprotein sulfotransferase 2 (TPST2), transcript variant 1



  "NM_001008566" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TPST2
Synonyms TANGO13B; TPST-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204969 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCTGTCGGTGCGGAGGGTGCTGCTGGCAGCCGGCTGCGCCCTGGTCCTGGTGCTGGCGGTTCAGC
TGGGACAGCAGGTGCTAGAGTGCCGGGCGGTGCTGGCGGGCCTGCGGAGCCCCCGGGGGGCCATGCGGCC
TGAGCAGGAGGAGCTGGTGATGGTGGGCACCAACCACGTGGAATACCGCTATGGCAAGGCCATGCCGCTC
ATCTTCGTGGGTGGCGTGCCTCGCAGTGGCACCACGTTGATGCGCGCCATGCTGGACGCCCACCCTGAGG
TGCGCTGCGGCGAGGAGACCCGCATCATCCCGCGCGTGCTGGCCATGCGCCAGGCCTGGTCCAAGTCTGG
CCGTGAGAAGCTGCGGCTGGATGAGGCGGGGGTGACGGATGAGGTGCTGGACGCCGCCATGCAGGCCTTC
ATCCTGGAGGTGATTGCCAAGCACGGAGAGCCGGCCCGCGTGCTCTGCAACAAGGACCCATTTACGCTCA
AGTCCTCGGTCTACCTGTCGCGCCTGTTCCCCAACTCCAAGTTCCTGCTGATGGTGCGGGACGGCCGGGC
CTCCGTGCACTCCATGATCACGCGCAAAGTCACCATTGCGGGCTTTGACCTCAGCAGCTACCGTGACTGC
CTCACCAAGTGGAACAAGGCCATCGAGGTGATGTACGCCCAGTGCATGGAGGTAGGCAAGGAGAAGTGCT
TGCCTGTGTACTACGAGCAGCTGGTGCTGCACCCCAGGCGCTCACTCAAGCTCATCCTCGACTTCCTCGG
CATCGCCTGGAGCGACGCTGTCCTCCACCATGAAGACCTCATTGGCAAGCCCGGTGGTGTCTCCCTGTCC
AAGATCGAGCGGTCCACGGACCAGGTCATCAAGCCTGTTAACCTGGAAGCGCTCTCCAAGTGGACTGGCC
ACATCCCTGGGGATGTGGTGCGGGACATGGCCCAGATCGCCCCCATGCTGGCTCAGCTCGGCTATGACCC
TTATGCAAACCCCCCCAACTATGGCAACCCTGACCCCTTCGTCATCAACAACACACAGCGGGTCTTGAAA
GGGGACTATAAAACACCAGCCAATCTGAAAGGATATTTTCAGGTGAACCAGAACAGCACCTCCTCCCACT
TAGGAAGCTCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204969 protein sequence
Red=Cloning site Green=Tags(s)

MRLSVRRVLLAAGCALVLVLAVQLGQQVLECRAVLAGLRSPRGAMRPEQEELVMVGTNHVEYRYGKAMPL
IFVGGVPRSGTTLMRAMLDAHPEVRCGEETRIIPRVLAMRQAWSKSGREKLRLDEAGVTDEVLDAAMQAF
ILEVIAKHGEPARVLCNKDPFTLKSSVYLSRLFPNSKFLLMVRDGRASVHSMITRKVTIAGFDLSSYRDC
LTKWNKAIEVMYAQCMEVGKEKCLPVYYEQLVLHPRRSLKLILDFLGIAWSDAVLHHEDLIGKPGGVSLS
KIERSTDQVIKPVNLEALSKWTGHIPGDVVRDMAQIAPMLAQLGYDPYANPPNYGNPDPFVINNTQRVLK
GDYKTPANLKGYFQVNQNSTSSHLGSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001008566
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001008566.1, NP_001008566.1
RefSeq Size 2018 bp
RefSeq ORF 1134 bp
Locus ID 8459
UniProt ID O60704
Cytogenetics 22q12.1
Protein Families Transmembrane
MW 41.9 kDa
Gene Summary The protein encoded by this gene catalyzes the O-sulfation of tyrosine residues within acidic regions of proteins. The encoded protein is a type II integral membrane protein found in the Golgi body. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]

Other Versions

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