PHF22 (INTS12) (NM_020395) Human Tagged ORF Clone

CAT#: RC204838

INTS12 (Myc-DDK-tagged)-Human integrator complex subunit 12 (INTS12), transcript variant 1

Product Images

Circular map for RC204838

• 

  Western blot validation of overexpression lysate (Cat# LY428117) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227792 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF22
• Synonyms: INT12; PHF22; SBBI22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204838 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTACTGTGAACTTGGAACTTGATCCCATTTTTTTGAAAGCACTAGGTTTCTTGCATTCAAAGA
  GTAAAGATTCTGCTGAAAAGCTAAAAGCACTGCTTGATGAATCTTTGGCTCGGGGCATTGATTCCAGTTA
  CCGTCCATCTCAAAAGGATGTGGAGCCACCCAAAATTTCAAGCACAAAAAACATTTCCATTAAGCAAGAG
  CCCAAAATATCATCCAGTCTTCCTTCTGGTAATAATAATGGCAAGGTCCTCACAACTGAAAAGGTAAAGA
  AGGAAGCTGAAAAGAGACCTGCTGATAAAATGAAATCAGACATCACTGAAGGAGTTGATATTCCAAAGAA
  ACCTAGATTGGAGAAACCAGAAACACAGTCATCTCCCATTACTGTCCAAAGTAGCAAGGATTTACCTATG
  GCTGACCTTTCCAGTTTTGAGGAGACCAGTGCTGATGATTTTGCCATGGAGATGGGATTGGCCTGCGTTG
  TTTGTAGGCAAATGATGGTGGCATCTGGCAATCAATTAGTAGAATGTCAGGAGTGCCATAATCTCTACCA
  CCGAGATTGTCATAAACCCCAGGTGACAGACAAGGAAGCGAATGACCCTCGCCTGGTGTGGTATTGTGCC
  CGATGTACCAGACAAATGAAAAGAATGGCTCAAAAAACTCAGAAACCACCGCAGAAACCAGCCCCTGCAG
  TTGTTTCTGTAACTCCAGCTGTCAAAGATCCATTGGTTAAGAAACCAGAAACTAAACTGAAACAAGAGAC
  AACTTTTCTAGCGTTTAAGAGAACAGAAGTCAAGACATCCACAGTTATTTCAGGAAATTCTTCTAGTGCC
  AGCGTTTCCTCGTCAGTAACTAGTGGCTTAACTGGATGGGCAGCTTTTGCAGCCAAAACTTCCTCTGCTG
  GTCCTTCAACAGCAAAATTGAGTTCAACAACACAAAACAATACTGGGAAACCTGCTACTTCGTCAGCTAA
  CCAGAAACCTGTGGGTTTGACTGGTCTGGCAACATCATCCAAAGGTGGAATAGGTTCCAAAATAGGTTCC
  AATAACAGCACTACGCCCACTGTACCTTTAAAACCACCTCCACCTCTAACCTTGGGTAAAACTGGCCTTA
  GTCGCTCAGTTAGTTGTGACAATGTCAGCAAAGTAGGTCTTCCTAGTCCAAGTAGTTTAGTTCCAGGAAG
  CAGCAGCCAACTAAGTGGGAATGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACT
  ACTTCAGAATCCAGCAGCTCTCCCTCAGCATCCCTTAAAGGCCCAACTTCACAAGAATCACAGCTCAATG
  CTATGAAGCGATTACAGATGGTCAAGAAGAAAGCTGCCCAAAAGAAACTCAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204838 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATVNLELDPIFLKALGFLHSKSKDSAEKLKALLDESLARGIDSSYRPSQKDVEPPKISSTKNISIKQE
  PKISSSLPSGNNNGKVLTTEKVKKEAEKRPADKMKSDITEGVDIPKKPRLEKPETQSSPITVQSSKDLPM
  ADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQECHNLYHRDCHKPQVTDKEANDPRLVWYCA
  RCTRQMKRMAQKTQKPPQKPAPAVVSVTPAVKDPLVKKPETKLKQETTFLAFKRTEVKTSTVISGNSSSA
  SVSSSVTSGLTGWAAFAAKTSSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGS
  NNSTTPTVPLKPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKT
  TSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020395
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020395.4
• RefSeq Size: 1768 bp
• RefSeq ORF: 1389 bp
• Locus ID: 57117
• UniProt ID: Q96CB8
• Cytogenetics: 4q24
• Domains: PHD
• Protein Families: Druggable Genome, Transcription Factors
• MW: 48.8 kDa
• Gene Summary: INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM, Mar 2008]