WDR77 (NM_024102) Human Tagged ORF Clone

CAT#: RC204532

WDR77 (Myc-DDK-tagged)-Human WD repeat domain 77 (WDR77)

Product Images

Circular map for RC204532

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY WDR77 (Cat# RC204532, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR77 (Cat# TA809827)(1:500). Positive lysates LY411336 (100ug) and LC411336 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411336) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204532 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR77
• Synonyms: HKMT1069; MEP-50; MEP50; Nbla10071; p44; p44/Mep50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204532 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAAGGAAACCCCACCCCCCCTAGTGCCCCCGGCGGCCCGGGAGTGGAATCTTCCCCCAAATGCGC
  CCGCCTGCATGGAACGGCAGTTGGAGGCTGCGCGGTACCGGTCCGATGGGGCGCTTCTCCTCGGGGCCTC
  CAGCCTGAGTGGGCGCTGCTGGGCCGGCTCCCTCTGGCTTTTTAAGGACCCCTGTGCCGCCCCCAACGAA
  GGCTTCTGCTCCGCCGGAGTCCAAACGGAGGCTGGAGTGGCTGACCTCACTTGGGTTGGGGAGAGAGGTA
  TTCTAGTGGCCTCCGATTCAGGTGCTGTTGAATTGTGGGAACTAGATGAGAATGAGACACTTATTGTCAG
  CAAGTTCTGCAAGTATGAGCATGATGACATTGTGTCTACAGTCAGTGTCTTGAGCTCTGGCACACAAGCT
  GTCAGTGGTAGCAAAGACATCTGCATCAAGGTTTGGGACCTTGCTCAGCAGGTGGTACTGAGTTCATACC
  GAGCTCATGCTGCTCAGGTCACTTGTGTTGCTGCCTCTCCTCACAAGGACTCTGTGTTTCTTTCATGCAG
  CGAGGACAATAGAATTTTACTCTGGGATACCCGCTGTCCCAAGCCAGCATCACAGATTGGCTGCAGTGCG
  CCTGGCTACCTTCCTACCTCGCTGGCTTGGCATCCTCAGCAAAGTGAAGTCTTTGTCTTTGGTGATGAGA
  ATGGGACAGTCTCCCTTGTGGACACCAAGAGTACAAGCTGTGTCCTGAGCTCAGCTGTACACTCCCAGTG
  TGTCACTGGGCTGGTGTTCTCCCCACACAGTGTTCCCTTCCTGGCCTCTCTCAGTGAAGACTGCTCACTT
  GCTGTGCTGGACTCAAGCCTTTCTGAGTTGTTTAGAAGCCAAGCCCACAGAGACTTTGTGAGAGATGCGA
  CTTGGTCCCCGCTCAATCACTCCCTGCTTACCACAGTGGGCTGGGACCATCAGGTCGTCCACCACGTTGT
  GCCCACAGAACCTCTCCCAGCCCCTGGACCTGCAAGTGTTACTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204532 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNE
  GFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQA
  VSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSA
  PGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSL
  AVLDSSLSELFRSQAHRDFVRDATWSPLNHSLLTTVGWDHQVVHHVVPTEPLPAPGPASVTE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024102
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024102.4
• RefSeq Size: 2428 bp
• RefSeq ORF: 1029 bp
• Locus ID: 79084
• UniProt ID: Q9BQA1
• Cytogenetics: 1p13.2
• Domains: WD40
• Protein Families: Stem cell - Pluripotency
• MW: 36.7 kDa
• Gene Summary: The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]