SLC25A12 (NM_003705) Human Tagged ORF Clone

CAT#: RC204475

SLC25A12 (Myc-DDK-tagged)-Human solute carrier family 25 (mitochondrial carrier, Aralar), member 12 (SLC25A12), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC204475

• 

  Western blot validation of overexpression lysate (Cat# LY401222) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204475 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC25A12
• Synonyms: AGC1; ARALAR; DEE39; EIEE39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204475 representing NM_003705
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTCAAGGTGCAGACAACTAAGCGAGGGGATCCTCATGAGTTAAGAAACATATTTCTACAGTATG
  CCAGTACTGAGGTTGATGGAGAGCGTTATATGACCCCAGAAGACTTTGTTCAGCGCTATCTTGGACTGTA
  TAATGATCCAAATAGTAACCCAAAGATCGTGCAGCTCTTGGCAGGAGTAGCTGATCAAACCAAGGATGGG
  TTGATCTCCTATCAAGAGTTTTTGGCATTTGAATCTGTTTTATGTGCTCCAGATTCCATGTTCATAGTGG
  CTTTCCAGTTGTTTGACAAGAGTGGAAATGGAGAGGTGACATTTGAAAATGTCAAAGAAATTTTTGGACA
  GACTATTATTCATCATCATATCCCTTTTAACTGGGATTGTGAATTTATCCGACTGCATTTTGGGCATAAC
  CGGAAGAAGCATCTTAACTACACAGAATTCACGCAGTTTCTCCAGGAGCTGCAATTGGAACATGCAAGAC
  AAGCCTTTGCACTCAAAGACAAAAGCAAAAGTGGCATGATTTCTGGTCTGGATTTCAGTGACATCATGGT
  TACCATTAGATCTCACATGCTTACTCCTTTTGTGGAGGAGAACTTAGTTTCAGCAGCTGGAGGAAGTATC
  TCACACCAGGTTAGCTTCTCCTACTTCAATGCATTTAACTCGTTACTGAATAACATGGAGCTTGTTCGTA
  AGATATATAGCACTCTAGCTGGCACAAGGAAAGATGTTGAAGTCACAAAGGAGGAATTTGCCCAGAGTGC
  CATACGCTATGGACAAGTCACACCACTAGAAATTGATATTCTATATCAGCTTGCAGACTTATATAATGCT
  TCAGGGCGCTTGACTTTGGCAGATATTGAGAGAATAGCCCCATTGGCTGAGGGGGCCTTACCTTACAACC
  TGGCAGAACTTCAGAGACAGCAGTCTCCTGGGTTAGGCAGGCCTATCTGGCTCCAGATTGCCGAGTCTGC
  TTACAGATTCACTCTGGGCTCAGTTGCTGGAGCTGTGGGAGCCACTGCAGTGTATCCTATAGATCTGGTG
  AAGACCCGAATGCAAAACCAGCGTGGCTCTGGCTCTGTTGTTGGGGAGCTAATGTACAAAAACAGCTTTG
  ACTGTTTTAAGAAAGTCTTGCGTTATGAGGGCTTCTTTGGACTCTACAGGGGTCTGATACCACAACTTAT
  AGGGGTTGCTCCAGAAAAGGCCATTAAACTGACTGTTAATGATTTTGTTCGGGACAAATTTACCAGAAGA
  GATGGCTCTGTTCCACTTCCAGCAGAAGTTCTTGCTGGAGGCTGTGCTGGAGGCTCTCAGGTCATTTTTA
  CCAACCCATTGGAGATAGTGAAGATTCGTCTGCAAGTAGCTGGAGAGATCACCACGGGACCCAGAGTCAG
  CGCCCTGAATGTGCTCCGGGACTTGGGAATTTTTGGTCTGTATAAGGGTGCCAAAGCGTGTTTCCTCCGA
  GACATTCCCTTCTCTGCAATCTATTTTCCTGTTTATGCTCATTGCAAACTACTTCTGGCTGATGAAAATG
  GACACGTGGGAGGTTTAAATCTTCTTGCAGCTGGAGCCATGGCAGGTGTCCCAGCTGCATCTCTGGTGAC
  CCCTGCTGATGTCATCAAGACAAGACTGCAGGTGGCTGCCCGCGCTGGCCAGACGACATACAGTGGTGTC
  ATCGACTGTTTCAGGAAGATTCTCCGGGAAGAAGGGCCCTCAGCATTTTGGAAAGGGACTGCAGCTCGAG
  TGTTTCGATCCTCTCCCCAGTTTGGTGTTACCTTGGTCACTTATGAACTTCTCCAGCGGTGGTTTTACAT
  TGATTTTGGAGGCCTCAAACCCGCTGGTTCAGAACCAACACCTAAGTCACGCATTGCAGACCTTCCTCCT
  GCCAACCCTGATCACATCGGTGGATACAGACTCGCCACAGCCACGTTTGCAGGCATCGAAAACAAATTTG
  GCCTTTATCTCCCGAAATTTAAGTCTCCTAGTGTTGCTGTGGTTCAGCCAAAGGCAGCAGTGGCAGCCAC
  TCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204475 representing NM_003705
  Red=Cloning site Green=Tags(s)
  
  MAVKVQTTKRGDPHELRNIFLQYASTEVDGERYMTPEDFVQRYLGLYNDPNSNPKIVQLLAGVADQTKDG
  LISYQEFLAFESVLCAPDSMFIVAFQLFDKSGNGEVTFENVKEIFGQTIIHHHIPFNWDCEFIRLHFGHN
  RKKHLNYTEFTQFLQELQLEHARQAFALKDKSKSGMISGLDFSDIMVTIRSHMLTPFVEENLVSAAGGSI
  SHQVSFSYFNAFNSLLNNMELVRKIYSTLAGTRKDVEVTKEEFAQSAIRYGQVTPLEIDILYQLADLYNA
  SGRLTLADIERIAPLAEGALPYNLAELQRQQSPGLGRPIWLQIAESAYRFTLGSVAGAVGATAVYPIDLV
  KTRMQNQRGSGSVVGELMYKNSFDCFKKVLRYEGFFGLYRGLIPQLIGVAPEKAIKLTVNDFVRDKFTRR
  DGSVPLPAEVLAGGCAGGSQVIFTNPLEIVKIRLQVAGEITTGPRVSALNVLRDLGIFGLYKGAKACFLR
  DIPFSAIYFPVYAHCKLLLADENGHVGGLNLLAAGAMAGVPAASLVTPADVIKTRLQVAARAGQTTYSGV
  IDCFRKILREEGPSAFWKGTAARVFRSSPQFGVTLVTYELLQRWFYIDFGGLKPAGSEPTPKSRIADLPP
  ANPDHIGGYRLATATFAGIENKFGLYLPKFKSPSVAVVQPKAAVAATQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003705
• ORF Size: 2034 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003705.5
• RefSeq Size: 2964 bp
• RefSeq ORF: 2037 bp
• Locus ID: 8604
• UniProt ID: O75746
• Cytogenetics: 2q31.1
• Domains: mito_carr, EFh
• Protein Families: Druggable Genome
• MW: 74.6 kDa
• Gene Summary: This gene encodes a calcium-binding mitochondrial carrier protein. The encoded protein localizes to the mitochondria and is involved in the exchange of aspartate for glutamate across the inner mitochondrial membrane. Polymorphisms in this gene may be associated with autism, and mutations in this gene may also be a cause of global cerebral hypomyelination. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2012]