SAV1 (NM_021818) Human Tagged ORF Clone

CAT#: RC204389

SAV1 (Myc-DDK-tagged)-Human salvador homolog 1 (Drosophila) (SAV1)

Product Images

Circular map for RC204389

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SAV1 (Cat# RC204389, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAV1(Cat# TA804764). Positive lysates LY402881 (100ug) and LC402881 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402881) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204389 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SAV1 protein (Cat# TP304389). The protein was produced from HEK293T cells transfected with SAV1 cDNA clone (Cat# RC204389) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SAV1
• Synonyms: SAV; WW45; WWP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204389 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCCGAAAGAAAACCAAAAACGAAGTGTCCAAGCCGGCCGAGGTGCAGGGGAAGTACGTGAAGA
  AGGAGACGTCGCCTCTGCTTCGGAATCTTATGCCTTCATTCATCCGGCATGGTCCAACAATTCCAAGACG
  AACTGATATCTGTCTTCCAGATTCAAGCCCTAATGCCTTTTCAACTTCTGGAGATGTAGTTTCAAGAAAC
  CAGAGTTTCCTTAGAACTCCAATTCAAAGAACACCTCATGAAATAATGAGAAGAGAAAGCAACAGATTAT
  CTGCACCTTCTTATCTTGCCAGAAGTCTAGCAGATGTCCCTAGAGAGTATGGTTCTTCTCAGTCATTTGT
  AACGGAAGTTAGTTTTGCTGTTGAAAATGGAGACTCTGGTTCCCGATATTATTATTCAGACAATTTTTTT
  GATGGTCAGAGAAAGCGGCCACTTGGAGATCGTGCACATGAAGACTACAGATATTATGAATACAACCATG
  ATCTCTTCCAAAGAATGCCACAGAATCAGGGGAGGCATGCTTCAGGTATTGGGAGAGTTGCTGCTACATC
  TTTAGGAAATTTGACTAACCATGGTTCTGAAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGGACA
  ATGAGAGGGAGAAAATATTATATAGATCATAACACAAATACAACTCACTGGAGCCATCCTCTTGAGCGAG
  AAGGACTTCCTCCTGGATGGGAACGAGTTGAGTCATCCGAATTTGGAACCTATTATGTAGATCACACAAA
  TAAGAAGGCCCAATACAGGCATCCCTGTGCTCCTAGTGTACCTCGGTATGATCAACCACCTCCTGTCACA
  TACCAGCCACAGCAAACTGAAAGAAATCAGTCCCTTCTGGTACCTGCAAATCCATATCATACTGCAGAAA
  TTCCTGACTGGCTTCAGGTTTACGCACGAGCCCCTGTGAAATATGACCACATTCTGAAGTGGGAACTCTT
  CCAGCTGGCTGACCTGGATACATACCAGGGAATGCTAAAGTTGCTCTTCATGAAAGAATTGGAGCAGATT
  GTTAAAATGTATGAAGCATACAGACAAGCCCTTCTTACAGAGTTGGAAAACCGAAAGCAGAGACAGCAGT
  GGTATGCCCAACAACATGGAAAAAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204389 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDICLPDSSPNAFSTSGDVVSRN
  QSFLRTPIQRTPHEIMRRESNRLSAPSYLARSLADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFF
  DGQRKRPLGDRAHEDYRYYEYNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWT
  MRGRKYYIDHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQPPPVT
  YQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQI
  VKMYEAYRQALLTELENRKQRQQWYAQQHGKNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021818
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021818.4
• RefSeq Size: 3141 bp
• RefSeq ORF: 1152 bp
• Locus ID: 60485
• UniProt ID: Q9H4B6
• Cytogenetics: 14q22.1
• Domains: WW
• MW: 44.6 kDa
• Gene Summary: WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein with two WW domains, a SARAH domain, and a coiled-coil region and is ubiquitously expressed in adult tissues. This protein binds to MST1 (mammalian sterile 20-like kinase 1) and promotes MST1-induced apoptosis. It has also been shown to bind to HAX1 (hematopoietic cell-specific protein 1 (HS1)-associated protein X-1) and to attenuate the anti-apoptotic effects of HAX1. Studies in human and mouse suggest this gene acts as a tumor suppressor. [provided by RefSeq, Aug 2012]