RPUSD4 (NM_032795) Human Tagged ORF Clone

CAT#: RC204352

RPUSD4 (Myc-DDK-tagged)-Human RNA pseudouridylate synthase domain containing 4 (RPUSD4), transcript variant 1

Product Images

Circular map for RC204352

• 

  Western blot validation of overexpression lysate (Cat# LY409829) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204352 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPUSD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204352 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCAGGTGGAGCGCGTCGGGCCCCTGGATCCGGGGAAACGGCCAAGGTTGCGGGAGTCTCT
  TCACTCTCGTCTCAAAGCCATTTTGTGCCGCTGCCGCTGCCTCTACGGCCATAAATGCCCAGAGATTAGC
  GGAGAAGCTCCGAGCCCAGAAACGGGAACAAGACACAAAGAAGGAGCCGGTGTCCACAAACGCTGTTCAG
  CGGAGAGTGCAAGAAATAGTGCGGTTCACACGGCAGCTGCAGCGAGTCCACCCCAACGTGCTTGCTAAGG
  CACTGACCCGAGGAATTCTCCACCAGGACAAGAACCTTGTGGTCATCAATAAGCCCTACGGTCTCCCTGT
  GCATGGTGGCCCTGGGGTCCAGCTCTGCATCACTGATGTACTACCTATCCTGGCAAAGATGCTTCATGGC
  CACAAGGCAGAGCCCTTGCATCTGTGCCACCGGCTGGACAAGGAAACCACAGGTGTAATGGTGTTGGCTT
  GGGACAAGGACATGGCACATCAAGTCCAAGAGTTGTTTAGAACCCGTCAGGTGGTGAAGAAGTACTGGGC
  CATCACTGTGCATGTCCCCATGCCCTCAGCAGGAGTCGTGGACATCCCCATTGTGGAGAAGGAGGCGCAA
  GGCCAGCAGCAACACCACAAGATGACATTGTCCCCGAGCTACCGCATGGACGATGGGAAAATGGTGAAAG
  TGCGGCGCAGCCGGAATGCGCAAGTTGCTGTAACTCAGTACCAGGTGCTCAGCAGCACTCTCTCCTCCGC
  CCTCGTGGAGCTCCAGCCCATCACTGGAATAAAACATCAGCTTCGAGTTCACTTGTCTTTTGGATTGGAT
  TGTCCAATCCTTGGTGATCACAAGTACTCAGACTGGAATAGGTTGGCCCCCCAGAAGCTGTCTGTGGGCA
  CCCTGAAGAAGCTGGGGCTAGAACAGTCGAAGGCCCGCTACATCCCCCTTCACCTGCACGCCCGGCAGCT
  GATCCTGCCTGCCCTGGGGTCCGGGAAGGAGGAACTCAACTTGGTCTGCAAACTTCCTCGCTTCTTTGTG
  CATTCCCTGCACCGCCTGCGTTTAGAGATGCCAAATGAGGATCAAAATGAGAACAATGAAGCCAAGTGTC
  TGGGAGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204352 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPRWSASGPWIRGNGQGCGSLFTLVSKPFCAAAAASTAINAQRLAEKLRAQKREQDTKKEPVSTNAVQ
  RRVQEIVRFTRQLQRVHPNVLAKALTRGILHQDKNLVVINKPYGLPVHGGPGVQLCITDVLPILAKMLHG
  HKAEPLHLCHRLDKETTGVMVLAWDKDMAHQVQELFRTRQVVKKYWAITVHVPMPSAGVVDIPIVEKEAQ
  GQQQHHKMTLSPSYRMDDGKMVKVRRSRNAQVAVTQYQVLSSTLSSALVELQPITGIKHQLRVHLSFGLD
  CPILGDHKYSDWNRLAPQKLSVGTLKKLGLEQSKARYIPLHLHARQLILPALGSGKEELNLVCKLPRFFV
  HSLHRLRLEMPNEDQNENNEAKCLGAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032795
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032795.3
• RefSeq Size: 2527 bp
• RefSeq ORF: 1134 bp
• Locus ID: 84881
• UniProt ID: Q96CM3
• Cytogenetics: 11q24.2
• Domains: PseudoU_synth_2
• MW: 42.2 kDa
• Gene Summary: Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of different mitochondrial RNA substrates. Acts on position 1397 in 16S mitochondrial ribosomal RNA (16S mt-rRNA). This modification is required for the assembly of 16S mt-rRNA into a functional mitochondrial ribosome (PubMed:27974379). Acts on position 39 in mitochondrial tRNA(Phe) (PubMed:28082677). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA, controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).[UniProtKB/Swiss-Prot Function]