THAP11 (NM_020457) Human Tagged ORF Clone

CAT#: RC204281

THAP11 (Myc-DDK-tagged)-Human THAP domain containing 11 (THAP11)

Product Images

Circular map for RC204281

• 

  Western blot validation of overexpression lysate (Cat# LY412462) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204281 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THAP11
• Synonyms: CTG-B43a; CTG-B45d; HRIHFB2206; RONIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204281 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGCTTTACGTGCTGCGTGCCAGGCTGCTACAACAACTCGCACCGGGACAAGGCGCTGCACTTCT
  ACACGTTTCCAAAGGACGCTGAGTTGCGGCGCCTCTGGCTCAAGAACGTGTCGCGTGCCGGCGTCAGTGG
  GTGCTTCTCCACCTTCCAGCCCACCACAGGCCACCGTCTCTGCAGCGTTCACTTCCAGGGCGGCCGCAAG
  ACCTACACGGTACGCGTCCCCACCATCTTCCCGCTGCGCGGCGTCAATGAGCGCAAAGTAGCGCGCAGAC
  CCGCTGGGGCCGCGGCCGCCCGCCGCAGGCAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAGCAACA
  GCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGTCCTCACCCTCTGCCTCCACTGCCCAG
  ACTGCCCAGCTGCAGCCGAACCTGGTATCTGCTTCCGCGGCCGTGCTTCTCACCCTTCAGGCCACTGTAG
  ACAGCAGTCAGGCTCCGGGATCCGTACAGCCGGCGCCCATCACTCCCACTGGAGAAGACGTGAAGCCCAT
  CGATCTCACAGTGCAAGTGGAGTTTGCAGCCGCAGAGGGCGCAGCCGCTGCGGCCGCCGCGTCGGAGTTA
  CAGGCTGCTACCGCAGGGCTGGAGGCTGCCGAGTGCCCTATGGGCCCCCAGTTGGTGGTGGTAGGGGAAG
  AGGGCTTCCCTGATACTGGCTCCGACCATTCGTACTCCTTGTCGTCAGGCACCACGGAGGAGGAGCTCCT
  GCGCAAGCTGAATGAGCAGCGGGACATCCTGGCTCTGATGGAAGTGAAGATGAAAGAGATGAAAGGCAGC
  ATTCGCCACCTGCGTCTCACTGAGGCCAAGCTGCGCGAAGAACTGCGTGAGAAGGATCGGCTGCTTGCCA
  TGGCTGTCATCCGCAAGAAGCACGGAATG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204281 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRK
  TYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQ
  TAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASEL
  QAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGS
  IRHLRLTEAKLREELREKDRLLAMAVIRKKHGM
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020457
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020457.1
• RefSeq Size: 1903 bp
• RefSeq ORF: 945 bp
• Locus ID: 57215
• UniProt ID: Q96EK4
• Cytogenetics: 16q22.1
• MW: 34.3 kDa
• Gene Summary: The protein encoded by this gene contains a THAP domain, which is a conserved DNA-binding domain that has striking similarity to the site-specific DNA-binding domain (DBD) of Drosophila P element transposases. [provided by RefSeq, Jul 2008]