CAVIN3 (NM_145040) Human Tagged ORF Clone

CAT#: RC204254

PRKCDBP (Myc-DDK-tagged)-Human protein kinase C, delta binding protein (PRKCDBP)

Product Images

Circular map for RC204254

• 

  Western blot validation of overexpression lysate (Cat# LY403419) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204254 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CAVIN3
• Synonyms: cavin-3; HSRBC; PRKCDBP; SRBC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204254 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGAGAGTGCGTTGGAGCCGGGGCCTGTGCCCGAGGCGCCGGCGGGGGGTCCCGTGCACGCCGTGA
  CGGTGGTGACCCTGCTGGAGAAGCTGGCCTCCATGCTGGAGACTCTGCGGGAGCGGCAGGGAGGCCTGGC
  TCGAAGGCAGGGAGGCCTGGCAGGGTCCGTGCGCCGCATCCAGAGCGGCCTGGGCGCTCTGAGTCGCAGC
  CACGACACCACCAGCAACACCTTGGCGCAGCTGCTGGCCAAGGCGGAGCGCGTGAGCTCGCACGCCAACG
  CCGCCCAAGAGCGCGCGGTGCGCCGCGCAGCCCAGGTGCAGCGGCTGGAGGCCAACCACGGGCTGCTGGT
  GGCGCGCGGGAAGCTCCACGTTCTGCTCTTCAAGGAGGAGGGTGAAGTCCCAGCCAGCGCTTTCCAGAAG
  GCACCAGAGCCCTTGGGCCCGGCGGACCAGTCCGAGCTGGGCCCAGAGCAGCTGGAGGCCGAAGTTGGAG
  AGAGCTCGGACGAGGAGCCGGTGGAGTCCAGGGCCCAGCGGCTGCGGCGCACCGGATTGCAGAAGGTACA
  GAGCCTCCGAAGGGCCCTTTCGGGCCGGAAAGGCCCTGCAGCGCCACCGCCCACCCCGGTCAAGCCGCCT
  CGCCTTGGGCCTGGCCGGAGCGCTGAAGCCCAGCCGGAAGCCCAGCCTGCGCTGGAGCCCACGCTGGAGC
  CAGAGCCTCCGCAGGACACCGAGGAAGATCCCGGGAGACCTGGGGCTGCCGAAGAAGCTCTGCTCCAAAT
  GGAGAGTGTAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204254 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRESALEPGPVPEAPAGGPVHAVTVVTLLEKLASMLETLRERQGGLARRQGGLAGSVRRIQSGLGALSRS
  HDTTSNTLAQLLAKAERVSSHANAAQERAVRRAAQVQRLEANHGLLVARGKLHVLLFKEEGEVPASAFQK
  APEPLGPADQSELGPEQLEAEVGESSDEEPVESRAQRLRRTGLQKVQSLRRALSGRKGPAAPPPTPVKPP
  RLGPGRSAEAQPEAQPALEPTLEPEPPQDTEEDPGRPGAAEEALLQMESVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145040
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145040.1
• RefSeq Size: 1039 bp
• RefSeq ORF: 786 bp
• Locus ID: 112464
• UniProt ID: Q969G5
• Cytogenetics: 11p15.4
• Protein Families: Druggable Genome
• MW: 27.6 kDa
• Gene Summary: The protein encoded by this gene was identified as a binding protein of the protein kinase C, delta (PRKCD). The expression of this gene in cultured cell lines is strongly induced by serum starvation. The expression of this protein was found to be down-regulated in various cancer cell lines, suggesting the possible tumor suppressor function of this protein. [provided by RefSeq, Jul 2008]