TID1 (DNAJA3) (NM_005147) Human Tagged ORF Clone

CAT#: RC204240

DNAJA3 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3), transcript variant 1

Product Images

Circular map for RC204240

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  Western blot validation of overexpression lysate (Cat# LY417489) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204240 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DNAJA3 protein (Cat# TP304240). The protein was produced from HEK293T cells transfected with DNAJA3 cDNA clone (Cat# RC204240) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TID1
• Synonyms: HCA57; hTID-1; TID1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204240 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGCGGTGCTCCACACGCTGGTTGCTGGTGGTTGTGGGGACCCCGCGGCTGCCGGCTATATCGG
  GTAGAGGGGCCCGGCCGCCCAGGGAGGGCGTGGTGGGGGCATGGCTGAGCCGCAAGCTGAGCGTCCCCGC
  CTTTGCGTCTTCCCTGACCTCTTGCGGCCCCCGAGCGCTGCTGACATTGAGACCTGGTGTCAGCCTCACA
  GGAACAAAACATTACCCTTTCATTTGTACTGCCTCCTTCCACACGAGTGCCCCTTTGGCCAAAGAAGATT
  ATTATCAGATATTAGGAGTGCCTCGAAATGCCAGCCAGAAAGAGATCAAGAAAGCCTATTATCAGCTTGC
  CAAGAAGTATCACCCTGACACAAATAAGGATGATCCCAAAGCCAAGGAGAAGTTCTCCCAGCTGGCAGAA
  GCCTATGAGGTTTTGAGTGATGAGGTGAAGAGGAAGCAGTACGATGCCTACGGCTCTGCAGGCTTCGATC
  CTGGGGCCAGCGGCTCCCAGCATAGCTACTGGAAGGGAGGCCCCACTGTGGACCCCGAGGAGCTGTTCAG
  GAAGATCTTTGGCGAGTTCTCATCCTCTTCATTTGGAGATTTCCAGACCGTGTTTGATCAGCCTCAGGAA
  TACTTCATGGAGTTGACATTCAATCAAGCTGCAAAGGGGGTCAACAAGGAGTTCACCGTGAACATCATGG
  ACACGTGTGAGCGCTGCAACGGCAAGGGGAACGAGCCCGGCACCAAGGTGCAGCATTGCCACTACTGTGG
  CGGCTCCGGCATGGAAACCATCAACACAGGCCCTTTTGTGATGCGTTCCACGTGTAGGAGATGTGGTGGC
  CGCGGCTCCATCATCATATCGCCCTGTGTGGTCTGCAGGGGAGCAGGACAAGCCAAGCAGAAAAAGCGAG
  TGATGATCCCTGTGCCTGCAGGAGTCGAGGATGGCCAGACCGTGAGGATGCCTGTGGGAAAAAGGGAAAT
  TTTCATTACGTTCAGGGTGCAGAAAAGCCCTGTGTTCCGGAGGGACGGCGCAGACATCCACTCCGACCTC
  TTTATTTCTATAGCTCAGGCTCTTCTTGGGGGAACAGCCAGAGCCCAGGGCCTGTACGAGACGATCAACG
  TGACGATCCCCCCTGGGACTCAGACAGACCAGAAGATTCGGATGGGTGGGAAAGGCATCCCCCGGATTAA
  CAGCTACGGCTACGGAGACCACTACATCCACATCAAGATACGAGTTCCAAAGAGGCTAACGAGCCGGCAG
  CAGAGCCTGATCCTGAGCTACGCCGAGGACGAGACAGATGTGGAGGGGACGGTGAACGGCGTCACCCTCA
  CCAGCTCTGGTGGCAGCACCATGGATAGCTCCGCAGGAAGCAAGGCTAGGCGTGAGGCTGGGGAGGACGA
  GGAGGGATTCCTTTCCAAACTTAAGAAAATGTTTACCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204240 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLT
  GTKHYPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAE
  AYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQE
  YFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGG
  RGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDL
  FISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQ
  QSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005147
• ORF Size: 1440 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005147.3, NP_005138.2
• RefSeq Size: 2780 bp
• RefSeq ORF: 1443 bp
• Locus ID: 9093
• UniProt ID: Q96EY1
• Cytogenetics: 16p13.3
• Domains: DnaJ_CXXCXGXG, DnaJ, DnaJ_C
• MW: 52.5 kDa
• Gene Summary: This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]