TID1 (DNAJA3) (NM_005147) Human Untagged Clone

CAT#: SC324462

DNAJA3 (untagged)-Human DnaJ (Hsp40) homolog, subfamily A, member 3 (DNAJA3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TID1
• Synonyms: HCA57; hTID-1; TID1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005147.3 GATGGCTGCGCGGTGCTCCACACGCTGGTTGCTGGTGGTTGTGGGGACCCCGCGGCTGCC
  GGCTATATCGGGTAGAGGGGCCCGGCCGCCCAGGGAGGGCGTGGTGGGGGCATGGCTGAG
  CCGCAAGCTGAGCGTCCCCGCCTTTGCGTCTTCCCTGACCTCTTGCGGCCCCCGAGCGCT
  GCTGACATTGAGACCTGGTGTCAGCCTCACAGGAACAAAACATTACCCTTTCATTTGTAC
  TGCCTCCTTCCACACGAGTGCCCCTTTGGCCAAAGAAGATTATTATCAGATATTAGGAGT
  GCCTCGAAATGCCAGCCAGAAAGAGATCAAGAAAGCCTATTATCAGCTTGCCAAGAAGTA
  TCACCCTGACACAAATAAGGATGATCCCAAAGCCAAGGAGAAGTTCTCCCAGCTGGCAGA
  AGCCTATGAGGTTTTGAGTGATGAGGTGAAGAGGAAGCAGTACGATGCCTACGGCTCTGC
  AGGCTTCGATCCTGGGGCCAGCGGCTCCCAGCATAGCTACTGGAAGGGAGGCCCCACTGT
  GGACCCCGAGGAGCTGTTCAGGAAGATCTTTGGCGAGTTCTCATCCTCTTCATTTGGAGA
  TTTCCAGACCGTGTTTGATCAGCCTCAGGAATACTTCATGGAGTTGACATTCAATCAAGC
  TGCAAAGGGGGTCAACAAGGAGTTCACCGTGAACATCATGGACACGTGTGAGCGCTGCAA
  CGGCAAGGGGAACGAGCCCGGCACCAAGGTGCAGCATTGCCACTACTGTGGCGGCTCCGG
  CATGGAAACCATCAACACAGGCCCTTTTGTGATGCGTTCCACGTGTAGGAGATGTGGTGG
  CCGCGGCTCCATCATCATATCGCCCTGTGTGGTCTGCAGGGGAGCAGGACAAGCCAAGCA
  GAAAAAGCGAGTGATGATCCCTGTGCCTGCAGGAGTCGAGGATGGCCAGACCGTGAGGAT
  GCCTGTGGGAAAAAGGGAAATTTTCATTACGTTCAGGGTGCAGAAAAGCCCTGTGTTCCG
  GAGGGACGGCGCAGACATCCACTCCGACCTCTTTATTTCTATAGCTCAGGCTCTTCTTGG
  GGGAACAGCCAGAGCCCAGGGCCTGTACGAGACGATCAACGTGACGATCCCCCCTGGGAC
  TCAGACAGACCAGAAGATTCGGATGGGTGGGAAAGGCATCCCCCGGATTAACAGCTACGG
  CTACGGAGACCACTACATCCACATCAAGATACGAGTTCCAAAGAGGCTAACGAGCCGGCA
  GCAGAGCCTGATCCTGAGCTACGCCGAGGACGAGACAGATGTGGAGGGGACGGTGAACGG
  CGTCACCCTCACCAGCTCTGGTGGCAGCACCATGGATAGCTCCGCAGGAAGCAAGGCTAG
  GCGTGAGGCTGGGGAGGACGAGGAGGGATTCCTTTCCAAACTTAAGAAAATGTTTACCTC
  ATGATATCCCAGCCGAGGAAAAAGATCCACTGGAAACTAGGCCGGGAAGCAGCAGCCCCT
  CCAAGGGCCAGGGCACCTGGGAGACGGGAGGATTCCAGAACAGCAGCACTGAGCTCCCAC
  CCGCAGAGCCTCTGGACGGCCTTGGCAACAGCAAAATCATGGGACAACACCTCTCTCCAC
  GGAAAGGTCACAGTGGACAGCCCGGGCAGTAGGATGCAGCCCCAGAGGCTGGTGGCAGTT
  TCCTGTCCATTGGTAGGTGACGGCCCCTGGCTCAGGCAGAGGGAGATGGTTAGACTCTTG
  CAGGGCTAAAACTCTAATTTGGAATTGAATATTGTGGATATCTTAGTTAAAGGCCATGCT
  TACAGCTTAGAAATGAAGCCTTAAGCTGCATCAAGTTACGAAGTGATTAATTTCCTTCTC
  AGCAAACCTCCGGGAGGTTCCAGAATGAGTTCTTCCTGACAGGTTGTCTTCACTGGGAGC
  GTGGGGCCCCCAGGCCCCACCAGCACCGTCCTCCCCTAATGAGGGGCCCTGCCGAGGCAT
  CAGCTGCTCTGCTCAGTTAGTTTTTATTCCCGGGGTACCAAGCAGCTGCACAGTCGGTGC
  CTGGGAGGCACGTAGAGGCCCAGAGAGTCCCTGGGGGTTCTGCTCTGACCGTGTGGGTGG
  TGATCCTTGTCAGGATGTACAGTCCTTGCTCCCACCCCATCCAGGATGGCCGCCTGTCCC
  TGACTATTGAGTCCTGTTGTTGTAAGCCAGGCATGGAGGGCTCCTGCCCTTCTGCTGAGC
  CACAGCCCATTGCAGCACTGTGCTGGCCAGACTTCAGCTGCCTTGGGAACTGAAGCCCTG
  CCACTGTTGCTAGTCAGGGGCTTGGTTCTCCCACTTACACTGTTGACATCTATTTTCTGA
  AGTGTGTTTAAATTATTCAGTGCTAATCATTGTTTTTTCCTTTGTAAATGTTGATTCAGA
  AAAGGAAAGCACAGGCTAAGCAGTTGAAGGTTCCCCACCATTCAGTGAGAGCAGAACCCC
  CATTCCCCAGCCTCTGCTGGTAGCATGTCGCAGTTTCCATGTGTTTCAGGATCTTCGGGC
  TGTCGTTAGACAGGTTAATGAAGAACACTTCTCAACAGTTTCCTTTTTGTTTTCCTTTAT
  AATTCACTAAAATAAAGCATCTATTAGTGTCTGATTTAGGAATGTAAAATGATTCTGTAT
  TAATGTAAATAAGATTATCTATTGCAAAAAGATATTTCAAACCTAAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_005147
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005147.3, NP_005138.2
• RefSeq Size: 2704 bp
• RefSeq ORF: 1443 bp
• Locus ID: 9093
• UniProt ID: Q96EY1
• Cytogenetics: 16p13.3
• Domains: DnaJ_CXXCXGXG, DnaJ, DnaJ_C
• Gene Summary: This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as TID1L).