DIPK1B (NM_152421) Human Tagged ORF Clone

CAT#: RC204222

FAM69B (Myc-DDK-tagged)-Human family with sequence similarity 69, member B (FAM69B)

Product Images

Circular map for RC204222

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DIPK1B
• Synonyms: C9orf136; FAM69B; pp6977
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204222 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGGCTGCGGCGCCTGGCGCACCTGGTGCTCTTCTGCCCCTTCTCCAAGCGCCTGCAGGGCCGGC
  TCCCAGGCCTCAGGGTCCGCTGCATCTTCCTGGCCTGGCTGGGCGTCTTTGCAGGCAGCTGGCTGGTGTA
  CGTGCACTACTCGTCCTACTCGGAGCGCTGTCGCGGCCATGTCTGCCAGGTGGTCATTTGTGACCAGTAC
  CGCAAGGGGATCATCTCGGGCTCCGTCTGCCAGGACCTGTGTGAGCTGCATATGGTGGAGTGGAGGACCT
  GCCTCTCGGTGGCCCCGGGCCAGCAGGTGTACAGCGGGCTCTGGCGGGACAAGGATGTAACCATCAAGTG
  TGGCATTGAGGAGACCCTCGACTCCAAGGCCCGGTCGGATGCGGCCCCCCGGCGGGAGCTGGTACTGTTT
  GACAAGCCCACCCGGGGCACCTCCATCAAGGAATTCCGGGAGATGACCCTCGGCTTCCTCAAGGCGAACC
  TGGGAGACCTGCCTTCCCTGCCGGCGCTGGTTGGCCAGGTCCTGCTCATGGCTGACTTCAACAAGGACAA
  CCGGGTGTCCCTGGCGGAAGCCAAGTCCGTGTGGGCCCTGCTGCAGCGTAACGAGTTCCTGCTGCTGCTG
  TCCCTGCAGGAGAAGGAGCACGCCTCCAGACTGCTGGGCTACTGTGGGGACCTCTACCTCACCGAGGGCG
  TGCCGCATGGCGCCTGGCACGCGGCCGCCCTTCCACCCCTGTTGCGCCCACTGCTGCCGCCTGCCCTGCA
  GGGTGCTCTCCAGCAGTGGCTGGGGCCTGCGTGGCCTTGGCGGGCCAAGATCGCCATCGGCCTGCTGGAG
  TTCGTGGAGGAGCTCTTCCACGGCTCTTACGGGACTTTCTACATGTGTGAGACCACACTGGCCAACGTGG
  GCTACACAGCCACCTACGACTTCAAGATGGCCGACCTGCAGCAGGTGGCACCCGAGGCCACCGTGCGCCG
  CTTCCTGCAGGGCCGCCGCTGCGAGCACAGCACCGACTGCACCTACGGGCGCGACTGCAGGGCCCCGTGT
  GACAGGCTCATGAGGCAGTGCAAGGGCGACCTCATCCAGCCCAACCTGGCCAAGGTGTGCGCACTGCTAC
  GGGGCTACCTGCTGCCTGGCGCGCCCGCCGACCTCCGCGAGGAGCTGGGCACACAGCTGCGCACCTGTAC
  CACGCTGAGCGGGCTGGCCAGCCAGGTGGAGGCCCATCACTCGCTGGTGCTCAGCCACCTCAAGACTCTG
  CTCTGGAAGAAGATCTCCAACACCAAGTACTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204222 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRLRRLAHLVLFCPFSKRLQGRLPGLRVRCIFLAWLGVFAGSWLVYVHYSSYSERCRGHVCQVVICDQY
  RKGIISGSVCQDLCELHMVEWRTCLSVAPGQQVYSGLWRDKDVTIKCGIEETLDSKARSDAAPRRELVLF
  DKPTRGTSIKEFREMTLGFLKANLGDLPSLPALVGQVLLMADFNKDNRVSLAEAKSVWALLQRNEFLLLL
  SLQEKEHASRLLGYCGDLYLTEGVPHGAWHAAALPPLLRPLLPPALQGALQQWLGPAWPWRAKIAIGLLE
  FVEELFHGSYGTFYMCETTLANVGYTATYDFKMADLQQVAPEATVRRFLQGRRCEHSTDCTYGRDCRAPC
  DRLMRQCKGDLIQPNLAKVCALLRGYLLPGAPADLREELGTQLRTCTTLSGLASQVEAHHSLVLSHLKTL
  LWKKISNTKYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152421
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152421.2, NP_689634.1
• RefSeq Size: 2334 bp
• RefSeq ORF: 1296 bp
• Locus ID: 138311
• UniProt ID: Q5VUD6
• Cytogenetics: 9q34.3
• Protein Families: Transmembrane
• MW: 48.6 kDa
• Gene Summary: This gene encodes a member of the FAM69 family of cysteine-rich type II transmembrane proteins. These proteins localize to the endoplasmic reticulum but their specific functions are unknown. [provided by RefSeq, Nov 2011]