MTRF1L (NM_019041) Human Tagged ORF Clone

CAT#: RC204221

MTRF1L (Myc-DDK-tagged)-Human mitochondrial translational release factor 1-like (MTRF1L), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC204221

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MTRF1L (Cat# RC204221, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTRF1L(Cat# TA501067). Positive lysates LY402734 (100ug) and LC402734 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402734) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204221 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTRF1L protein (Cat# TP304221). The protein was produced from HEK293T cells transfected with MTRF1L cDNA clone (Cat# RC204221) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTRF1L
• Synonyms: HMRF1L; MRF1L; mtRF1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204221 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTCCCGGGTTCTGTGGGGCGCTGCCCGGTGGCTCTGGCCCCGCCGGGCCGTTGGCCCAGCCCGCC
  GGCCCCTGAGCTCCGGTAGCCCGCCGCTGGAGGAGCTGTTCGCCCGGGGCGGGCCCTTGCGGACCTTCCT
  CGAGCGCCAGGCGGGGTCTGAAGCCCATTTGAAGGTCAGGAGGCCCGAGTTGCTGGCGGTGATCAAACTG
  CTGAACGAGAAGGAGCAGGAGCTGCGGGAGACTGAGCACTTGCTGCACGATGAGAATGAAGATTTAAGGA
  AACTTGCAGAGAATGAAATCACTTTGTGTCAAAAAGAAATAACTCAGCTGAAGCATCAGATTATCTTACT
  TTTGGTTCCCTCAGAAGAAACAGATGAAAATGATTTGATCCTGGAAGTAACTGCAGGAGTTGGAGGTCAG
  GAGGCAATGTTGTTTACATCAGAGATATTTGATATGTATCAGCAATATGCTGCATTTAAAAGATGGCATT
  TTGAAACCCTGGAATATTTTCCAAGTGAACTAGGTGGCCTTAGACATGCATCTGCCAGCATTGGGGGTTC
  AGAAGCCTATAGGCACATGAAATTTGAAGGAGGTGTTCACAGAGTACAAAGAGTGCCAAAGACAGAAAAG
  CAAGGCCGCGTCCATACTAGCACCATGACTGTAGCAATATTACCCCAGCCTACTGAGATTAATCTGGTGA
  TTAATCCGAAAGATTTGAGAATTGACACTAAGCGAGCCAGTGGAGCTGGGGGGCAGCATGTAAATACCAC
  GGACAGTGCTGTCCGGATAGTTCATCTTCCAACAGGTGTTGTTTCTGAATGTCAACAAGAGAGATCTCAG
  CTGAAAAATAAAGAGCTGGCTATGACAAAGTTACGTGCAAAACTGTACAGCATGCATCTAGAAGAAGAAA
  TAAATAAAAGACAGAATGCTAGAAAAATTCAGATTGGAAGTAAAGGAAGATCAGAGAAAATAAGAACATA
  TAATTTTCCACAGAACCGGGTCACAGATCACAGAATAAACAAGACGCTGCATGATCTTGAAACTTTTATG
  CAAGGAGATTATCTACTGGATGAACTTGTACAGTCATTGAAGGAATACGCCGATTATGAATCTTTAGTAG
  AAATTATTTCCCAAAAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204221 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSRVLWGAARWLWPRRAVGPARRPLSSGSPPLEELFARGGPLRTFLERQAGSEAHLKVRRPELLAVIKL
  LNEKEQELRETEHLLHDENEDLRKLAENEITLCQKEITQLKHQIILLLVPSEETDENDLILEVTAGVGGQ
  EAMLFTSEIFDMYQQYAAFKRWHFETLEYFPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEK
  QGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQ
  LKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFM
  QGDYLLDELVQSLKEYADYESLVEIISQKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019041
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019041.4
• RefSeq Size: 3815 bp
• RefSeq ORF: 1143 bp
• Locus ID: 54516
• UniProt ID: Q9UGC7
• Cytogenetics: 6q25.2
• Domains: RF-1, PCRF
• MW: 43.5 kDa
• Gene Summary: The protein encoded by this gene plays a role in mitochondrial translation termination, and is thought to be a release factor that is involved in the dissociation of the complete protein from the final tRNA, the ribosome, and the cognate mRNA. This protein acts upon UAA and UAG stop codons, but has no in vitro activity against UGA, which encodes tryptophan in human mitochondrion, or, the mitochondrial non-cognate stop codons, AGA and AGG. This protein shares sequence similarity to bacterial release factors. Pseudogenes of this gene are found on chromosomes 4, 8, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]