MOCOS (NM_017947) Human Tagged ORF Clone

CAT#: RC204120

MOCOS (Myc-DDK-tagged)-Human molybdenum cofactor sulfurase (MOCOS)

Product Images

Circular map for RC204120

• 

  Western blot validation of overexpression lysate (Cat# LY413431) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204120 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MOCOS protein (Cat# TP304120). The protein was produced from HEK293T cells transfected with MOCOS cDNA clone (Cat# RC204120) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOCOS
• Synonyms: HMCS; MCS; MOS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204120 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGCGGCGGCGGAGTCAGGGCGGGAGCTGTGGACCTTCGCGGGTTCCCGGGACCCGAGCGCAC
  CGCGGCTAGCCTACGGCTACGGCCCGGGCAGCCTGCGCGAGCTGCGGGCGCGCGAGTTCAGCCGCCTGGC
  AGGAACTGTCTATCTTGACCATGCAGGTGCCACCTTGTTCTCCCAGAGCCAGCTCGAAAGCTTCACTAGT
  GATCTCATGGAAAACACTTATGGTAATCCTCACAGCCAGAACATCAGCAGCAAGCTCACCCATGACACTG
  TGGAGCAGGTGCGCTACAGAATCCTGGCGCACTTCCACACCACCGCAGAAGACTACACTGTGATCTTCAC
  TGCCGGGAGCACGGCTGCTCTCAAACTGGTGGCAGAGGCCTTTCCATGGGTGTCCCAGGGCCCAGAGAGC
  AGTGGGAGTCGCTTCTGTTACCTCACCGACAGCCACACCTCCGTAGTGGGTATGAGGAACGTGACCATGG
  CTATAAATGTCATATCCATCCCGGTCAGGCCAGAGGACCTGTGGTCTGCAGAGGAACGTGGTGCTTCAGC
  CAGCAACCCAGACTGCCAGCTGCCGCATCTCTTCTGCTACCCAGCTCAGAGTAACTTTTCTGGAGTCAGA
  TACCCCCTGTCCTGGATAGAAGAGGTCAAGTCTGGGCGGTTGCGCCCTGTGAGCACGCCTGGGAAGTGGT
  TTGTGCTGCTGGATGCAGCCTCCTACGTGAGCACCTCGCCTTTGGACCTGTCAGCTCACCAGGCCGACTT
  TGTCCCCATCTCCTTCTATAAGATCTTCGGGTTTCCTACAGGCCTGGGCGCTCTGCTGGTCCATAATCGT
  GCGGCTCCTCTACTGAGGAAGACCTACTTTGGAGGAGGGACAGCCTCTGCGTACCTAGCAGGAGAAGACT
  TCTACATCCCGAGGCAGTCGGTAGCTCAGAGGTTTGAAGATGGCACCATCTCATTCCTTGATGTTATCGC
  GCTAAAACATGGATTTGACACCCTAGAGCGCCTCACAGGTGGAATGGAGAATATAAAGCAGCACACCTTC
  ACCTTGGCTCAGTATACCTACATGGCCCTGTCCTCTCTCCAGTACCCCAATGGAGCCCCTGTGGTGCGGA
  TTTACAGCGATTCTGAGTTCAGCAGCCCTGAGGTTCAGGGCCCGATCATCAATTTTAATGTGCTGGATGA
  CAAAGGGAACATCATTGGTTACTCCCAGGTGGACAAAATGGCCAGTCTTTACAACATCCACCTGCGAACT
  GGCTGCTTCTGTAACACTGGGGCCTGCCAGAGGCACCTGGGCATAAGCAACGAGATGGTCAGGAAGCATT
  TTCAGGCTGGTCATGTCTGTGGGGACAATATGGACCTCATAGATGGGCAGCCCACAGGATCTGTGAGGAT
  TTCATTTGGATACATGTCGACGCTGGATGATGTCCAGGCCTTTCTTAGGTTCATCATAGACACTCGCCTG
  CACTCATCAGGGGACTGGCCTGTCCCTCAGGCCCATGCTGACACCGGGGAGACTGGAGCCCCATCAGCAG
  ACAGCCAGGCTGATGTTATACCTGCTGTCATGGGCAGACGTAGCCTCTCGCCTCAGGAAGATGCCCTCAC
  AGGCTCCAGGGTTTGGAACAACTCGTCTACTGTGAATGCTGTGCCTGTGGCCCCACCTGTGTGTGATGTC
  GCCAGAACCCAGCCGACTCCTTCAGAGAAAGCTGCAGGAGTCCTGGAGGGGGCCCTTGGGCCACATGTTG
  TCACTAACCTTTATCTCTATCCAATCAAATCCTGTGCTGCATTTGAGGTGACCAGGTGGCCTGTAGGAAA
  CCAAGGGCTGCTATATGACCGGAGCTGGATGGTTGTGAATCACAATGGTGTTTGCCTGAGTCAGAAGCAG
  GAACCCCGGCTCTGCCTGATCCAGCCCTTCATCGACTTGCGGCAAAGGATCATGGTCATCAAAGCCAAAG
  GGATGGAGCCTATAGAGGTGCCTCTTGAGGAAAATAGTGAACGGACTCAGATTCGCCAAAGCAGGGTCTG
  TGCTGACAGAGTAAGTACTTATGATTGTGGAGAAAAAATTTCAAGCTGGTTGTCAACATTTTTTGGCCGT
  CCTTGTCATTTGATCAAACAAAGTTCAAACTCTCAAAGGAATGCAAAGAAGAAACATGGAAAAGATCAAC
  TTCCTGGTACAATGGCCACCCTTTCTCTGGTGAATGAGGCACAGTATCTGCTGATCAACACATCCAGTAT
  TTTGGAACTTCACCGGCAACTAAACACCAGTGATGAGAATGGAAAGGAGGAATTATTCTCACTGAAGGAT
  CTCAGCTTGCGTTTTCGTGCCAATATTATTATCAATGGAAAAAGGGCTTTTGAAGAAGAGAAATGGGATG
  AGATTTCAATTGGCTCTTTGCGTTTCCAGGTTTTGGGGCCTTGTCACAGATGCCAGATGATTTGCATCGA
  CCAGCAAACTGGGCAACGAAACCAGCATGTTTTCCAAAAACTTTCTGAGAGTCGTGAAACAAAGGTGAAC
  TTTGGCATGTACCTGATGCATGCATCATTGGATTTATCCTCCCCATGTTTCCTGTCTGTAGGATCTCAGG
  TGCTCCCTGTGTTGAAAGAGAATGTGGAAGGTCATGATTTACCTGCATCTGAGAAACACCAGGATGTTAC
  CTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204120 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGAAAESGRELWTFAGSRDPSAPRLAYGYGPGSLRELRAREFSRLAGTVYLDHAGATLFSQSQLESFTS
  DLMENTYGNPHSQNISSKLTHDTVEQVRYRILAHFHTTAEDYTVIFTAGSTAALKLVAEAFPWVSQGPES
  SGSRFCYLTDSHTSVVGMRNVTMAINVISIPVRPEDLWSAEERGASASNPDCQLPHLFCYPAQSNFSGVR
  YPLSWIEEVKSGRLRPVSTPGKWFVLLDAASYVSTSPLDLSAHQADFVPISFYKIFGFPTGLGALLVHNR
  AAPLLRKTYFGGGTASAYLAGEDFYIPRQSVAQRFEDGTISFLDVIALKHGFDTLERLTGGMENIKQHTF
  TLAQYTYMALSSLQYPNGAPVVRIYSDSEFSSPEVQGPIINFNVLDDKGNIIGYSQVDKMASLYNIHLRT
  GCFCNTGACQRHLGISNEMVRKHFQAGHVCGDNMDLIDGQPTGSVRISFGYMSTLDDVQAFLRFIIDTRL
  HSSGDWPVPQAHADTGETGAPSADSQADVIPAVMGRRSLSPQEDALTGSRVWNNSSTVNAVPVAPPVCDV
  ARTQPTPSEKAAGVLEGALGPHVVTNLYLYPIKSCAAFEVTRWPVGNQGLLYDRSWMVVNHNGVCLSQKQ
  EPRLCLIQPFIDLRQRIMVIKAKGMEPIEVPLEENSERTQIRQSRVCADRVSTYDCGEKISSWLSTFFGR
  PCHLIKQSSNSQRNAKKKHGKDQLPGTMATLSLVNEAQYLLINTSSILELHRQLNTSDENGKEELFSLKD
  LSLRFRANIIINGKRAFEEEKWDEISIGSLRFQVLGPCHRCQMICIDQQTGQRNQHVFQKLSESRETKVN
  FGMYLMHASLDLSSPCFLSVGSQVLPVLKENVEGHDLPASEKHQDVTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017947
• ORF Size: 2664 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017947.3
• RefSeq Size: 2747 bp
• RefSeq ORF: 2667 bp
• Locus ID: 55034
• UniProt ID: Q96EN8
• Cytogenetics: 18q12.2
• MW: 98.2 kDa
• Gene Summary: This gene encodes an enzyme that sulfurates the molybdenum cofactor which is required for activation of the xanthine dehydrogenase (XDH) and aldehyde oxidase (AO) enzymes. XDH catalyzes the conversion of hypoxanthine to uric acid via xanthine, as well as the conversion of allopurinol to oxypurinol, and pyrazinamide to 5-hydroxy pyrazinamide. Mutations in this gene cause the metabolic disorder classical xanthinuria type II which is characterized by the loss of XDH/XO and AO enzyme activity, decreased levels of uric acid in the urine, increased levels of xanthine and hypoxanthine in the serum and urine, formation of xanthine stones in the urinary tract, and myositis due to tissue deposition of xanthine. [provided by RefSeq, Apr 2017]