POLR3K (NM_016310) Human Tagged ORF Clone
POLR3K (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)
|Product Name||POLR3K (NM_016310) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||C11; C11-RNP3; My010; RPC10; RPC11; RPC12.5|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC204102 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC204102 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||324 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_016310.1, NM_016310.2, NM_016310.3, NM_016310.4, NP_057394.1|
|RefSeq Size||834 bp|
|RefSeq ORF||327 bp|
|Protein Families||Transcription Factors|
|Protein Pathways||Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase|
|Gene Summary||This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17. [provided by RefSeq, Jul 2010]|
|cDNA Clone Resources|
|SC114310||POLR3K (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)||
|RG204102||POLR3K (GFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)||
|RC204102L3||Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K), Myc-DDK-tagged||
|RC204102L4||Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K), mGFP tagged||