POLR3K (NM_016310) Human Tagged ORF Clone
CAT#: RG204102
- TrueORF®
POLR3K (tGFP-tagged) - Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016310" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | POLR3K |
Synonyms | C11; C11-RNP3; HLD21; My010; RPC10; RPC11; RPC12.5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204102 representing NM_016310
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGTTCTGCCCCGGCTGCGGGAACGGGCTGATCGTGGAGGAGGGACAACGCTGCCACCGCTTCG CCTGCAACACGTGCCCCTACGTGCACAACATCACCCGCAAGGTAACAAATCGGAAGTACCCAAAACTGAA AGAAGTGGATGATGTGCTTGGTGGAGCAGCTGCCTGGGAGAATGTTGACTCTACTGCAGAGTCGTGTCCC AAATGCGAACATCCTCGTGCTTACTTCATGCAGCTTCAGACCCGCTCTGCAGATGAGCCGATGACCACCT TCTACAAGTGCTGCAATGCTCAGTGTGGACACCGCTGGAGGGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204102 representing NM_016310
Red=Cloning site Green=Tags(s) MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCP KCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016310 |
ORF Size | 324 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016310.5 |
RefSeq Size | 796 bp |
RefSeq ORF | 327 bp |
Locus ID | 51728 |
UniProt ID | Q9Y2Y1 |
Cytogenetics | 16p13.3 |
Domains | TFIIS, RNA_POL_M_15KD |
Protein Families | Transcription Factors |
Protein Pathways | Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
Gene Summary | This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription. Pseudogenes of this gene are found on chromosomes 13 and 17.[provided by RefSeq, Jul 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204102 | POLR3K (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K) |
USD 150.00 |
|
RC204102L3 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K), Myc-DDK-tagged |
USD 450.00 |
|
RC204102L4 | Lenti ORF clone of Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K), mGFP tagged |
USD 450.00 |
|
SC114310 | POLR3K (untagged)-Human polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa (POLR3K) |
USD 150.00 |
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