C14orf172 (TRMT61A) (NM_152307) Human Tagged ORF Clone

CAT#: RC204071

TRMT61A (Myc-DDK-tagged)-Human tRNA methyltransferase 61 homolog A (S. cerevisiae) (TRMT61A)

Product Images

Circular map for RC204071

• 

  Western blot validation of overexpression lysate (Cat# LY407663) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204071 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C14orf172
• Synonyms: C14orf172; GCD14; Gcd14p; hTRM61; TRM61
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204071 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCGTGGCATACGAGGAGCTGATCAAGGAGGGTGACACGGCCATCCTGTCACTGGGCCATGGTG
  CAATGGTGGCAGTGCGTGTGCAGCGTGGGGCACAGACCCAGACCCGGCATGGTGTCCTGCGGCACTCAGT
  TGACCTTATCGGCCGCCCCTTCGGCTCCAAGGTGACGTGCGGCCGAGGTGGCTGGGTGTATGTGCTGCAC
  CCCACGCCCGAGCTCTGGACGCTGAACCTGCCGCACCGCACGCAGATCCTCTACTCCACAGACATCGCCC
  TCATCACCATGATGTTGGAGCTTCGGCCCGGCTCTGTGGTCTGTGAGTCTGGCACCGGCAGTGGCTCTGT
  GTCCCACGCCATCATCCGCACCATTGCACCCACGGGTCACCTGCACACGGTGGAGTTCCACCAGCAGCGG
  GCAGAGAAGGCCCGGGAGGAGTTCCAGGAGCACCGTGTGGGCCGCTGGGTGACTGTGCGCACCCAGGACG
  TGTGCCGCAGTGGCTTTGGCGTGAGCCACGTGGCCGACGCCGTCTTCCTGGACATCCCATCACCCTGGGA
  GGCCGTGGGCCACGCCTGGGACGCCCTCAAGGTCGAAGGCGGGCGCTTCTGCTCCTTCTCACCGTGCATC
  GAGCAGGTGCAACGCACATGCCAGGCGCTGGCAGCGCGCGGCTTCTCAGAGCTGAGCACCCTGGAGGTGC
  TGCCACAGGTCTACAACGTGCGCACTGTCAGCCTGCCACCGCCCGACCTGGGCACAGGCACAGATGGCCC
  TGCCGGCTCCGACACCAGCCCCTTCCGCAGCGGCACGCCCATGAAGGAGGCCGTGGGCCACACCGGCTAC
  CTGACCTTCGCCACCAAGACCCCAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204071 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLH
  PTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQR
  AEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCI
  EQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGY
  LTFATKTPG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152307
• ORF Size: 867 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152307.2, NP_689520.2
• RefSeq Size: 3257 bp
• RefSeq ORF: 870 bp
• Locus ID: 115708
• UniProt ID: Q96FX7
• Cytogenetics: 14q32.33
• Protein Families: Druggable Genome
• MW: 31.4 kDa
• Gene Summary: Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA (PubMed:16043508). Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries (PubMed:29107537, PubMed:29072297).[UniProtKB/Swiss-Prot Function]