C14orf172 (TRMT61A) Rabbit Polyclonal Antibody

CAT#: TA382854

TRMT61A Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TRMT61A antibody (TA382854) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Western blot analysis of extracts of various cell lines, using TRMT61A antibody (TA382854) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:1000 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 31kDa
• Gene Name: tRNA methyltransferase 61A
• Database Link:
  Entrez Gene 115708 Human
  UniProt ID Q96FX7
• Background: Catalytic subunit of tRNA (adenine-N(1--methyltransferase, which catalyzes the formation of N(1-methyladenine at position 58 (m1A58 in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1-methyltransferase complex, which mediates methylation of adenosine residues at the N(1 position of a small subset of mRNAs: N(1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.
• Synonyms: C14orf172; FLJ40452; GCD14; Gcd14p; hTRM61; TRM61