PLEKHO1 (NM_016274) Human Tagged ORF Clone

CAT#: RC203969

PLEKHO1 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family O member 1 (PLEKHO1)

Product Images

Circular map for RC203969

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  Western blot validation of overexpression lysate (Cat# LY414081) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203969 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PLEKHO1 protein (Cat# TP303969). The protein was produced from HEK293T cells transfected with PLEKHO1 cDNA clone (Cat# RC203969) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLEKHO1
• Synonyms: CKIP-1; CKIP1; JBP; OC120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203969 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGAAGAACAATTCCGCCAAGCGGGGACCTCAGGATGGAAACCAGCAGCCTGCACCGCCCGAGA
  AGGTCGGCTGGGTCCGGAAATTCTGCGGGAAAGGGATTTTCAGGGAGATTTGGAAAAACCGCTATGTGGT
  GCTGAAAGGGGACCAGCTCTACATCTCTGAGAAGGAGGTAAAAGATGAGAAAAATATTCAAGAGGTATTT
  GACCTGAGTGACTATGAGAAGTGTGAAGAGCTCCGGAAGTCCAAGAGCAGGAGCAAGAAAAATCATAGCA
  AGTTTACTCTTGCCCACTCCAAACAGCCCGGTAACACGGCACCCAACCTGATCTTCCTGGCAGTGAGTCC
  AGAAGAGAAGGAATCGTGGATCAATGCCCTCAACTCTGCCATCACCCGAGCCAAGAACCGTATCTTGGAT
  GAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCATCCCACTCGAGACAGGGCAAAAATCCAGCACTCCC
  GCCGCCCCCCAACAAGGGGACACCTAATGGCTGTGGCTTCCACCTCTACCTCGGATGGGATGCTGACCTT
  GGACTTGATCCAAGAGGAAGACCCTTCCCCTGAGGAACCAACCTCTTGTGCTGAGAGCTTTCGGGTTGAC
  CTGGACAAGTCTGTGGCCCAGCTGGCAGGGAGCCGGCGGAGAGCGGACTCAGACCGCATCCAGCCCTCCG
  CAGACCGGGCAAGCAGTCTCTCCCGACCTTGGGAAAAAACAGACAAAGGGGCCACCTACACCCCCCAGGC
  ACCCAAGAAGTTGACGCCCACAGAGAAAGGCCGCTGCGCCTCCCTGGAGGAGATCCTATCTCAGCGGGAT
  GCTGCCTCTGCCCGCACCCTCCAGCTGCGGGCTGAGGAACCCCCAACCCCTGCCCTCCCCAACCCGGGGC
  AGCTGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTGGAGGAGACTCAGGAGCTTCTGGCAGAGGTTCA
  GGGACTGGGAGATGGGAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTCAGAGCAG
  CTGCTGCTGGAGACGGAACGGCTGCTGGGAGAGGCATCATCGAATTGGAGCCAGGCAAAGAGGGTGCTGC
  AGGAGGTCAGGGAGCTGAGAGACCTGTACAGACAGATGGACCTGCAGACCCCGGACTCCCACCTCAGACA
  GACCACCCCGCACAGTCAGTACCGGAAGAGCCTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203969 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMKKNNSAKRGPQDGNQQPAPPEKVGWVRKFCGKGIFREIWKNRYVVLKGDQLYISEKEVKDEKNIQEVF
  DLSDYEKCEELRKSKSRSKKNHSKFTLAHSKQPGNTAPNLIFLAVSPEEKESWINALNSAITRAKNRILD
  EVTVEEDSYLAHPTRDRAKIQHSRRPPTRGHLMAVASTSTSDGMLTLDLIQEEDPSPEEPTSCAESFRVD
  LDKSVAQLAGSRRRADSDRIQPSADRASSLSRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRD
  AASARTLQLRAEEPPTPALPNPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQ
  LLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016274
• ORF Size: 1227 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016274.6
• RefSeq Size: 1627 bp
• RefSeq ORF: 1230 bp
• Locus ID: 51177
• UniProt ID: Q53GL0
• Cytogenetics: 1q21.2
• Domains: PH
• MW: 46.2 kDa
• Gene Summary: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.[UniProtKB/Swiss-Prot Function]