gamma Adaptin (AP1G1) (NM_001030007) Human Tagged ORF Clone

CAT#: RC203954

AP1G1 (Myc-DDK-tagged)-Human adaptor-related protein complex 1, gamma 1 subunit (AP1G1), transcript variant 1

Product Images

Circular map for RC203954

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  Western blot validation of overexpression lysate (Cat# LY422287) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203954 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AP1G1 protein (Cat# TP303954). The protein was produced from HEK293T cells transfected with AP1G1 cDNA clone (Cat# RC203954) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: gamma Adaptin
• Synonyms: ADTG; CLAPG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203954 representing NM_001030007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCCCCATCAGATTGCGGGAGCTGATCCGGACCATCCGGACAGCCCGAACCCAAGCTGAAGAAC
  GAGAAATGATCCAGAAAGAATGTGCTGCAATCCGGTCATCTTTTAGAGAAGAAGACAATACATACCGATG
  TCGGAATGTGGCAAAATTACTGTATATGCACATGCTGGGCTACCCTGCTCACTTTGGACAGTTGGAGTGC
  TTCAAGCTTATTGCCTCTCAAAAATTTACAGACAAACGCATTGGCTATTTAGGGGCAATGCTGCTGTTAG
  ATGAAAGACAAGATGTCCATCTTCTCATGACCAACTGTATCAAGAATGATCTTAATCATAGCACGCAATT
  CGTACAGGGGTTAGCACTTTGTACCCTCGGCTGCATGGGCTCCTCAGAGATGTGCAGAGATCTTGCAGGA
  GAGGTAGAGAAGCTCCTGAAAACCTCCAACTCTTACTTAAGAAAAAAGGCAGCACTGTGTGCTGTTCATG
  TCATCAGGAAAGTTCCTGAACTTATGGAGATGTTTTTACCAGCAACAAAAAATTTATTGAATGAGAAGAA
  CCATGGTGTCCTCCACACATCTGTAGTCCTCCTCACAGAAATGTGTGAGCGAAGCCCAGACATGCTTGCG
  CATTTCAGAAAGAATGAAAAGCTTGTGCCCCAATTAGTTCGTATTTTAAAGAACCTCATCATGTCCGGAT
  ATTCACCAGAACATGATGTTTCTGGTATCAGTGACCCCTTTTTGCAGGTACGAATTTTGCGGTTATTAAG
  AATTTTAGGACGAAATGATGATGATTCAAGTGAAGCTATGAATGATATATTAGCACAGGTTGCCACTAAT
  ACTGAGACTAGTAAAAATGTAGGAAATGCTATTCTTTATGAAACGGTTTTGACTATCATGGATATTAAGT
  CAGAGAGTGGATTGCGAGTCCTAGCCATAAATATCCTGGGTCGTTTCTTATTGAACAATGACAAGAATAT
  TAGATATGTGGCTCTGACATCTTTGTTGAAGACTGTACAGACAGATCATAATGCAGTACAGAGGCACAGA
  AGCACAATTGTGGACTGTCTTAAAGATTTGGATGTCTCAATAAAACGGCGTGCAATGGAATTGAGTTTTG
  CCCTGGTAAATGGGAATAATATCCGAGGCATGATGAAAGAATTACTTTATTTTCTGGATTCGTGTGAGCC
  AGAATTTAAAGCAGACTGTGCATCTGGAATCTTTCTTGCTGCAGAAAAGTATGCACCTTCCAAACGATGG
  CATATAGACACAATTATGCGTGTTTTGACAACGGCAGGAAGTTATGTTCGTGATGATGCAGTCCCCAATT
  TAATCCAGTTAATAACTAATAGTGTGGAGATGCATGCCTATACTGTCCAGCGCCTGTACAAAGCAATTCT
  TGGTGATTATTCTCAACAACCTTTGGTACAAGTGGCTGCATGGTGTATAGGTGAATATGGTGATCTTCTT
  GTATCTGGCCAGTGTGAAGAGGAAGAGCCTATTCAGGTAACAGAGGATGAAGTGTTGGATATTTTAGAAA
  GTGTCCTAATCTCTAATATGTCCACCTCTGTGACACGAGGTTATGCCCTCACTGCCATTATGAAGCTTTC
  CACTCGATTCACTTGTACTGTAAACCGAATTAAGAAAGTGGTTTCCATCTACGGAAGCAGCATTGATGTG
  GAACTCCAGCAGAGGGCAGTAGAATATAATGCACTTTTCAAGAAATATGACCACATGAGGTCTGCCCTAC
  TTGAGAGAATGCCTGTCATGGAAAAAGTGACCACAAATGGCCCTACTGAGATTGTGCAGACAAATGGAGA
  GACAGAACCAGCTCCACTAGAGACCAAACCGCCACCCTCTGGGCCACAGCCCACCAGCCAGGCCAATGAT
  TTATTGGATTTGTTGGGAGGAAATGACATAACACCTGTTATTCCAACTGCGCCTACAAGCAAACCATCTT
  CTGCTGGTGGAGAACTTCTTGATTTGCTGGGAGACATCAACCTTACAGGTGCTCCAGCTGCTGCTCCTGC
  CCCTGCCTCAGTCCCACAGATATCCCAGCCCCCCTTCTTGTTGGATGGGCTTTCATCACAGCCTCTCTTC
  AATGATATTGCTGCAGGCATCCCCTCCATCACAGCATACAGTAAGAATGGCTTGAAGATAGAATTCACCT
  TTGAACGGTCAAATACCAACCCCAGTGTAACAGTGATAACGATACAGGCCTCCAACAGCACAGAGCTAGA
  TATGACGGACTTTGTTTTCCAAGCTGCAGTACCAAAGACATTCCAGCTGCAGCTCTTGTCTCCTAGCAGC
  AGCATTGTCCCAGCATTTAACACGGGGACCATCACACAAGTCATTAAAGTTCTGAACCCTCAGAAGCAAC
  AGCTGCGAATGCGGATCAAGCTTACATATAATCACAAGGGCTCAGCAATGCAAGATCTAGCAGAGGTGAA
  CAACTTTCCCCCTCAGTCCTGGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203954 representing NM_001030007
  Red=Cloning site Green=Tags(s)
  
  MPAPIRLRELIRTIRTARTQAEEREMIQKECAAIRSSFREEDNTYRCRNVAKLLYMHMLGYPAHFGQLEC
  FKLIASQKFTDKRIGYLGAMLLLDERQDVHLLMTNCIKNDLNHSTQFVQGLALCTLGCMGSSEMCRDLAG
  EVEKLLKTSNSYLRKKAALCAVHVIRKVPELMEMFLPATKNLLNEKNHGVLHTSVVLLTEMCERSPDMLA
  HFRKNEKLVPQLVRILKNLIMSGYSPEHDVSGISDPFLQVRILRLLRILGRNDDDSSEAMNDILAQVATN
  TETSKNVGNAILYETVLTIMDIKSESGLRVLAINILGRFLLNNDKNIRYVALTSLLKTVQTDHNAVQRHR
  STIVDCLKDLDVSIKRRAMELSFALVNGNNIRGMMKELLYFLDSCEPEFKADCASGIFLAAEKYAPSKRW
  HIDTIMRVLTTAGSYVRDDAVPNLIQLITNSVEMHAYTVQRLYKAILGDYSQQPLVQVAAWCIGEYGDLL
  VSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDV
  ELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQAND
  LLDLLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQISQPPFLLDGLSSQPLF
  NDIAAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSS
  SIVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001030007
• ORF Size: 2475 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001030007.1, NP_001025178.1
• RefSeq Size: 6850 bp
• RefSeq ORF: 2478 bp
• Locus ID: 164
• UniProt ID: O43747
• Cytogenetics: 16q22.2
• Protein Pathways: Lysosome
• MW: 91.5 kDa
• Gene Summary: Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]