ESAM (NM_138961) Human Tagged ORF Clone

CAT#: RC203885

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ESAM (Myc-DDK-tagged)-Human endothelial cell adhesion molecule (ESAM)



  "NM_138961" in other vectors (7)

USD 433.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name ESAM (NM_138961) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ESAM
Synonyms W117m
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC203885 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTTCCCTCCCGGGGCCCCTGGTGACCAACTTGCTGCGGTTTTTGTTCCTGGGGCTGAGTGCCCTCG
CGCCCCCCTCGCGGGCCCAGCTGCAACTGCACTTGCCCGCCAACCGGTTGCAGGCGGTGGAGGGAGGGGA
AGTGGTGCTTCCAGCGTGGTACACCTTGCACGGGGAGGTGTCTTCATCCCAGCCATGGGAGGTGCCCTTT
GTGATGTGGTTCTTCAAACAGAAAGAAAAGGAGGATCAGGTGTTGTCCTACATCAATGGGGTCACAACAA
GCAAACCTGGAGTATCCTTGGTCTACTCCATGCCCTCCCGGAACCTGTCCCTGCGGCTGGAGGGTCTCCA
GGAGAAAGACTCTGGCCCCTACAGCTGCTCCGTGAATGTGCAAGACAAACAAGGCAAATCTAGGGGCCAC
AGCATCAAAACCTTAGAACTCAATGTACTGGTTCCTCCAGCTCCTCCATCCTGCCGTCTCCAGGGTGTGC
CCCATGTGGGGGCAAACGTGACCCTGAGCTGCCAGTCTCCAAGGAGTAAGCCCGCTGTCCAATACCAGTG
GGATCGGCAGCTTCCATCCTTCCAGACTTTCTTTGCACCAGCATTAGATGTCATCCGTGGGTCTTTAAGC
CTCACCAACCTTTCGTCTTCCATGGCTGGAGTCTATGTCTGCAAGGCCCACAATGAGGTGGGCACTGCCC
AATGTAATGTGACGCTGGAAGTGAGCACAGGGCCTGGAGCTGCAGTGGTTGCTGGAGCTGTTGTGGGTAC
CCTGGTTGGACTGGGGTTGCTGGCTGGGCTGGTCCTCTTGTACCACCGCCGGGGCAAGGCCCTGGAGGAG
CCAGCCAATGATATCAAGGAGGATGCCATTGCTCCCCGGACCCTGCCCTGGCCCAAGAGCTCAGACACAA
TCTCCAAGAATGGGACCCTTTCCTCTGTCACCTCCGCACGAGCCCTCCGGCCACCCCATGGCCCTCCCAG
GCCTGGTGCATTGACCCCCACGCCCAGTCTCTCCAGCCAGGCCCTGCCCTCACCAAGACTGCCCACGACA
GATGGGGCCCACCCTCAACCAATATCCCCCATCCCTGGTGGGGTTTCTTCCTCTGGCTTGAGCCGCATGG
GTGCTGTGCCTGTGATGGTGCCTGCCCAGAGTCAAGCTGGCTCTCTGGTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203885 protein sequence
Red=Cloning site Green=Tags(s)

MISLPGPLVTNLLRFLFLGLSALAPPSRAQLQLHLPANRLQAVEGGEVVLPAWYTLHGEVSSSQPWEVPF
VMWFFKQKEKEDQVLSYINGVTTSKPGVSLVYSMPSRNLSLRLEGLQEKDSGPYSCSVNVQDKQGKSRGH
SIKTLELNVLVPPAPPSCRLQGVPHVGANVTLSCQSPRSKPAVQYQWDRQLPSFQTFFAPALDVIRGSLS
LTNLSSSMAGVYVCKAHNEVGTAQCNVTLEVSTGPGAAVVAGAVVGTLVGLGLLAGLVLLYHRRGKALEE
PANDIKEDAIAPRTLPWPKSSDTISKNGTLSSVTSARALRPPHGPPRPGALTPTPSLSSQALPSPRLPTT
DGAHPQPISPIPGGVSSSGLSRMGAVPVMVPAQSQAGSLV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138961
ORF Size 1170 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138961.3
RefSeq Size 1869 bp
RefSeq ORF 1173 bp
Locus ID 90952
Cytogenetics 11q24.2
Domains ig, IGc2, IG
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
MW 41.2 kDa
Gene Summary Can mediate aggregation most likely through a homophilic molecular interaction.[UniProtKB/Swiss-Prot Function]

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