ESAM (NM_138961) Human Tagged ORF Clone

CAT#: RC203885

ESAM (Myc-DDK-tagged)-Human endothelial cell adhesion molecule (ESAM)

Product Images

Circular map for RC203885

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  Western blot validation of overexpression lysate (Cat# LY408393) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203885 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ESAM protein (Cat# TP303885). The protein was produced from HEK293T cells transfected with ESAM cDNA clone (Cat# RC203885) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ESAM
• Synonyms: W117m
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203885 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTCCCTCCCGGGGCCCCTGGTGACCAACTTGCTGCGGTTTTTGTTCCTGGGGCTGAGTGCCCTCG
  CGCCCCCCTCGCGGGCCCAGCTGCAACTGCACTTGCCCGCCAACCGGTTGCAGGCGGTGGAGGGAGGGGA
  AGTGGTGCTTCCAGCGTGGTACACCTTGCACGGGGAGGTGTCTTCATCCCAGCCATGGGAGGTGCCCTTT
  GTGATGTGGTTCTTCAAACAGAAAGAAAAGGAGGATCAGGTGTTGTCCTACATCAATGGGGTCACAACAA
  GCAAACCTGGAGTATCCTTGGTCTACTCCATGCCCTCCCGGAACCTGTCCCTGCGGCTGGAGGGTCTCCA
  GGAGAAAGACTCTGGCCCCTACAGCTGCTCCGTGAATGTGCAAGACAAACAAGGCAAATCTAGGGGCCAC
  AGCATCAAAACCTTAGAACTCAATGTACTGGTTCCTCCAGCTCCTCCATCCTGCCGTCTCCAGGGTGTGC
  CCCATGTGGGGGCAAACGTGACCCTGAGCTGCCAGTCTCCAAGGAGTAAGCCCGCTGTCCAATACCAGTG
  GGATCGGCAGCTTCCATCCTTCCAGACTTTCTTTGCACCAGCATTAGATGTCATCCGTGGGTCTTTAAGC
  CTCACCAACCTTTCGTCTTCCATGGCTGGAGTCTATGTCTGCAAGGCCCACAATGAGGTGGGCACTGCCC
  AATGTAATGTGACGCTGGAAGTGAGCACAGGGCCTGGAGCTGCAGTGGTTGCTGGAGCTGTTGTGGGTAC
  CCTGGTTGGACTGGGGTTGCTGGCTGGGCTGGTCCTCTTGTACCACCGCCGGGGCAAGGCCCTGGAGGAG
  CCAGCCAATGATATCAAGGAGGATGCCATTGCTCCCCGGACCCTGCCCTGGCCCAAGAGCTCAGACACAA
  TCTCCAAGAATGGGACCCTTTCCTCTGTCACCTCCGCACGAGCCCTCCGGCCACCCCATGGCCCTCCCAG
  GCCTGGTGCATTGACCCCCACGCCCAGTCTCTCCAGCCAGGCCCTGCCCTCACCAAGACTGCCCACGACA
  GATGGGGCCCACCCTCAACCAATATCCCCCATCCCTGGTGGGGTTTCTTCCTCTGGCTTGAGCCGCATGG
  GTGCTGTGCCTGTGATGGTGCCTGCCCAGAGTCAAGCTGGCTCTCTGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203885 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MISLPGPLVTNLLRFLFLGLSALAPPSRAQLQLHLPANRLQAVEGGEVVLPAWYTLHGEVSSSQPWEVPF
  VMWFFKQKEKEDQVLSYINGVTTSKPGVSLVYSMPSRNLSLRLEGLQEKDSGPYSCSVNVQDKQGKSRGH
  SIKTLELNVLVPPAPPSCRLQGVPHVGANVTLSCQSPRSKPAVQYQWDRQLPSFQTFFAPALDVIRGSLS
  LTNLSSSMAGVYVCKAHNEVGTAQCNVTLEVSTGPGAAVVAGAVVGTLVGLGLLAGLVLLYHRRGKALEE
  PANDIKEDAIAPRTLPWPKSSDTISKNGTLSSVTSARALRPPHGPPRPGALTPTPSLSSQALPSPRLPTT
  DGAHPQPISPIPGGVSSSGLSRMGAVPVMVPAQSQAGSLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138961
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138961.3
• RefSeq Size: 1869 bp
• RefSeq ORF: 1173 bp
• Locus ID: 90952
• UniProt ID: Q96AP7
• Cytogenetics: 11q24.2
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration
• MW: 41.2 kDa
• Gene Summary: Can mediate aggregation most likely through a homophilic molecular interaction.[UniProtKB/Swiss-Prot Function]