TCTN2 (NM_024809) Human Tagged ORF Clone

CAT#: RC203860

TCTN2 (Myc-DDK-tagged)-Human tectonic family member 2 (TCTN2), transcript variant 1

Product Images

Circular map for RC203860

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TCTN2 (Cat# RC203860, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TCTN2(Cat# TA501477). Positive lysates LY411064 (100ug) and LC411064 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY411064) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203860 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TCTN2 protein (Cat# TP303860). The protein was produced from HEK293T cells transfected with TCTN2 cDNA clone (Cat# RC203860) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCTN2
• Synonyms: C12orf38; JBTS24; MKS8; TECT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203860 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCCAGCCTCCGGCCGCTCTTCTTTTGAGGCTTTTCCTTCTGCAGGGCATCCTGAGGCTTCTGT
  GGGGGGACCTGGCTTTCATCCCTCCTTTTATCCGAATGTCCGGCCCTGCGGTCAGCGCGTCCCTGGTCGG
  AGACACCGAGGGTGTGACCGTGTCCCTGGCAGTGCTGCAGGACGAGGCGGGAATATTGCCAATTCCGACG
  TGTGGAGTGCTGAACAATGAGACGGAAGACTGGAGCGTGACTGTGATCCCCGGTGCGAAGGTGTTGGAAG
  TGACAGTGAGGTGGAAGAGAGGTCTGGACTGGTGTTCCTCCAATGAGACAGATTCCTTCTCAGAGTCCCC
  CTGTATCCTCCAGACCCTTCTGGTTTCAGCATCTCATAATTCATCCTGTTCAGCACATCTACTCATTCAA
  GTGGAAATTTATGCCAACTCTTCTCTGACCCATAATGCCTCAGAGAACGTGACTGTCATTCCTAACCAGG
  TGTATCAGCCCCTTGGCCCTTGTCCTTGTAATTTAACAGCTGGAGCCTGTGATGTTCGCTGCTGCTGTGA
  CCAGGAATGCTCATCAAATTTAACAACGCTGTTCAGACGGTCCTGCTTCACCGGCGTGTTTGGAGGAGAC
  GTCAATCCTCCTTTTGATCAGCTCTGCTCTGCTGGGACGACGACACGTGGTGTCCCCGATTGGTTTCCCT
  TTCTGTGTGTGCAGTCCCCCCTTGCCAACACACCCTTCCTTGGTTACTTCTATCATGGTGCTGTTTCCCC
  CAAACAGGACTCTTCCTTTGAAGTATATGTGGATACTGACGCAAAAGACTTTGCAGACTTTGGTTACAAA
  CAAGGAGATCCCATTATGACTGTAAAGAAGGCATATTTTACTATTCCGCAGGTGTCCCTGGCTGGGCAGT
  GTATGCAGAACGCCCCAGTGGCATTTCTTCACAATTTTGATGTTAAATGCGTTACTAATTTGGAACTATA
  CCAAGAACGAGATGGTATTATCAATGCGAAGATAAAGAATGTTGCCTTAGGAGGCATAGTTACACCAAAA
  GTGATCTATGAGGAAGCAACTGACCTAGACAAATTCATCACCAATACAGAAACTCCTTTAAATAACGGAT
  CAACCCCTAGAATTGTGAATGTGGAAGAACATTATATTTTCAAATGGAATAATAATACCATCAGTGAAAT
  AAATGTTAAAATTTTTAGGGCAGAGATTAATGCCCACCAGAAAGGGATAATGACACAGAGATTTGTAGTA
  AAATTTTTAAGCTATAATAGTGGTAATGAAGAAGAATTATCTGGAAATCCAGGTTACCAACTTGGCAAGC
  CTGTCCGAGCTCTAAATATCAACAGGATGAATAATGTCACGACTTTACATCTTTGGCAATCGGCTGGAAG
  GGGTCTGTGTACATCAGCAACTTTCAAACCCATTTTATTTGGAGAAAATGTACTCTCTGGATGCCTGTTA
  GAAGTCGGGATTAATGAAAATTGTACTCAGCTCAGGGAGAATGCTGTTGAAAGACTTGATTCATTAATAC
  AAGCGACTCACGTTGCAATGAGAGGCAACTCCGATTACGCTGATCTTAGTGATGGCTGGCTCGAAATAAT
  ACGTGTAGATGCCCCTGATCCAGGTGCAGACCCGCTGGCTAGCAGTGTGAACGGCATGTGCCTGGATATT
  CCTGCTCACCTGAGCATCCGCATCCTCATCTCGGATGCTGGCGCGGTGGAAGGGATTACTCAGCAGGAGA
  TACTCGGTGTAGAGACAAGGTTCTCCTCAGTGAACTGGCAGTACCAGTGTGGGCTTACCTGTGAGCACAA
  GGCCGACCTTCTCCCTATCAGTGCATCCGTCCAGTTTATTAAAATTCCTGCACAGTTACCCCACCCCCTG
  ACAAGATTCCAGATCAATTATACAGAGTATGACTGCAACAGAAATGAGGTGTGTTGGCCGCAGCTTCTAT
  ATCCATGGACTCAGTATTATCAAGGGGAGCTGCATTCTCAGTGTGTTGCTAAGGGCTTACTGTTGCTGTT
  GTTCCTCACATTGGCCTTGTTCCTCAGCAACCCCTGGACCAGAATATGCAAAGCCTATAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203860 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGFQPPAALLLRLFLLQGILRLLWGDLAFIPPFIRMSGPAVSASLVGDTEGVTVSLAVLQDEAGILPIPT
  CGVLNNETEDWSVTVIPGAKVLEVTVRWKRGLDWCSSNETDSFSESPCILQTLLVSASHNSSCSAHLLIQ
  VEIYANSSLTHNASENVTVIPNQVYQPLGPCPCNLTAGACDVRCCCDQECSSNLTTLFRRSCFTGVFGGD
  VNPPFDQLCSAGTTTRGVPDWFPFLCVQSPLANTPFLGYFYHGAVSPKQDSSFEVYVDTDAKDFADFGYK
  QGDPIMTVKKAYFTIPQVSLAGQCMQNAPVAFLHNFDVKCVTNLELYQERDGIINAKIKNVALGGIVTPK
  VIYEEATDLDKFITNTETPLNNGSTPRIVNVEEHYIFKWNNNTISEINVKIFRAEINAHQKGIMTQRFVV
  KFLSYNSGNEEELSGNPGYQLGKPVRALNINRMNNVTTLHLWQSAGRGLCTSATFKPILFGENVLSGCLL
  EVGINENCTQLRENAVERLDSLIQATHVAMRGNSDYADLSDGWLEIIRVDAPDPGADPLASSVNGMCLDI
  PAHLSIRILISDAGAVEGITQQEILGVETRFSSVNWQYQCGLTCEHKADLLPISASVQFIKIPAQLPHPL
  TRFQINYTEYDCNRNEVCWPQLLYPWTQYYQGELHSQCVAKGLLLLLFLTLALFLSNPWTRICKAYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024809
• ORF Size: 2091 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024809.5
• RefSeq Size: 2930 bp
• RefSeq ORF: 2094 bp
• Locus ID: 79867
• UniProt ID: Q96GX1
• Cytogenetics: 12q24.31
• MW: 76.9 kDa
• Gene Summary: This gene encodes a type I membrane protein that belongs to the tectonic family. Studies in mice suggest that this protein may be involved in hedgehog signaling, and essential for ciliogenesis. Mutations in this gene are associated with Meckel syndrome type 8. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]