GDI1 (NM_001493) Human Tagged ORF Clone

CAT#: RC203824

GDI1 (Myc-DDK-tagged)-Human GDP dissociation inhibitor 1 (GDI1)

Product Images

Circular map for RC203824

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  Western blot validation of overexpression lysate (Cat# LY419896) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203824 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GDI1 protein (Cat# TP303824). The protein was produced from HEK293T cells transfected with GDI1 cDNA clone (Cat# RC203824) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GDI1 (Cat# RC203824, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GDI1(Cat# TA811044). Positive lysates LY419896 (100ug) and LC419896 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GDI1
• Synonyms: 1A; GDIL; MRX41; MRX48; OPHN2; RABGD1A; RABGDIA; XAP-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203824 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGAATACGATGTGATCGTGCTGGGGACCGGTCTCACCGAATGCATCCTGTCGGGCATCATGT
  CTGTGAACGGGAAGAAGGTGCTGCACATGGACCGGAACCCCTACTACGGGGGCGAGAGCTCCTCCATCAC
  ACCCCTGGAGGAGCTGTATAAGCGTTTTCAGTTGCTGGAGGGGCCCCCTGAGTCGATGGGCCGAGGCCGA
  GACTGGAATGTTGACCTGATTCCCAAATTCCTCATGGCTAACGGGCAGCTGGTAAAGATGCTACTGTATA
  CAGAGGTGACTCGCTACCTGGACTTCAAGGTGGTGGAGGGCAGCTTTGTCTACAAGGGGGGCAAGATCTA
  CAAAGTGCCGTCCACTGAGACTGAGGCCTTGGCTTCCAATCTGATGGGCATGTTTGAGAAACGGCGCTTC
  CGCAAGTTCCTGGTGTTTGTGGCAAACTTCGATGAGAATGACCCCAAGACCTTTGAGGGCGTTGACCCCC
  AGACTACCAGCATGCGTGACGTCTACCGGAAGTTTGATCTGGGCCAGGATGTCATCGATTTCACTGGCCA
  TGCCCTGGCGCTCTACCGCACTGATGACTACCTGGACCAGCCCTGCCTTGAGACCGTCAACCGCATCAAG
  TTGTACAGTGAGTCCCTGGCCCGGTATGGCAAGAGCCCATATTTATACCCGCTCTACGGCTTGGGCGAGC
  TGCCCCAGGGTTTTGCAAGATTGAGTGCCATCTATGGGGGGACATATATGCTGAACAAACCTGTGGATGA
  CATCATCATGGAGAACGGCAAGGTGGTGGGCGTGAAGTCTGAGGGAGAGGTGGCCCGCTGCAAGCAGCTG
  ATCTGTGACCCCAGCTACATCCCGGACCGTGTGCGGAAGGCTGGCCAGGTTATCCGCATCATCTGTATCC
  TTAGCCACCCCATCAAGAACACCAACGACGCCAACTCCTGCCAAATAATCATCCCCCAGAACCAGGTCAA
  CAGGAAGTCAGACATCTACGTGTGCATGATCTCCTATGCACACAACGTGGCGGCCCAGGGCAAGTACATA
  GCTATTGCCAGCACTACTGTGGAGACCACGGACCCTGAAAAGGAGGTGGAGCCGGCTCTGGAGCTGTTGG
  AGCCCATTGACCAGAAGTTTGTGGCTATCAGTGACTTGTATGAGCCCATTGATGATGGTTGTGAGAGCCA
  GGTGTTCTGTTCCTGCTCCTACGATGCCACCACACACTTTGAGACAACCTGCAACGACATCAAAGACATC
  TACAAACGCATGGCTGGCACGGCCTTTGACTTTGAGAACATGAAGCGCAAACAGAACGACGTCTTTGGAG
  AAGCTGAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203824 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESSSITPLEELYKRFQLLEGPPESMGRGR
  DWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVVEGSFVYKGGKIYKVPSTETEALASNLMGMFEKRRF
  RKFLVFVANFDENDPKTFEGVDPQTTSMRDVYRKFDLGQDVIDFTGHALALYRTDDYLDQPCLETVNRIK
  LYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPVDDIIMENGKVVGVKSEGEVARCKQL
  ICDPSYIPDRVRKAGQVIRIICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISYAHNVAAQGKYI
  AIASTTVETTDPEKEVEPALELLEPIDQKFVAISDLYEPIDDGCESQVFCSCSYDATTHFETTCNDIKDI
  YKRMAGTAFDFENMKRKQNDVFGEAEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001493
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001493.3
• RefSeq Size: 2505 bp
• RefSeq ORF: 1344 bp
• Locus ID: 2664
• UniProt ID: P31150
• Cytogenetics: Xq28
• Domains: GDI
• Protein Families: Druggable Genome
• MW: 50.6 kDa
• Gene Summary: GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific cognitive disability. [provided by RefSeq, Jul 2008]