C14orf169 (NM_024644) Human Tagged ORF Clone

CAT#: RC203678

C14orf169 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 169 (C14orf169)

Product Images

Circular map for RC203678

• 

  Western blot validation of overexpression lysate (Cat# LY411093) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203678 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C14orf169
• Synonyms: C14orf169; hsNO66; JMJD9; MAPJD; NO66; ROX; URLC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203678 representing NM_024644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGGCTCCAGGCCAGTGCAGGGCCGTTGAGGCGCGGGCGGCCGAAGCGCCGGCGCAAGCCCCAGC
  CACACAGCGGGTCGGTCCTGGCCCTGCCCTTGAGGTCCAGGAAGATACGAAAGCAGCTGCGAAGTGTTGT
  ATCCCGCATGGCAGCGCTGAGGACGCAGACGCTGCCTAGCGAGAACTCGGAGGAATCGAGGGTGGAGTCG
  ACGGCCGACGACCTGGGGGACGCGCTACCCGGTGGGGCGGCGGTGGCGGCCGTCCCGGACGCAGCCCGGC
  GAGAGCCATACGGCCACCTGGGGCCCGCAGAGCTGCTGGAGGCCTCGCCCGCCGCGCGCTCCCTGCAGAC
  CCCGTCGGCGCGCCTGGTGCCCGCTTCCGCGCCGCCCGCGCGCCTGGTGGAGGTGCCCGCCGCGCCGGTC
  CGGGTGGTGGAGACCTCGGCCCTGCTGTGCACCGCGCAACACTTAGCGGCCGTCCAGTCGTCCGGGGCCC
  CTGCGACGGCGTCGGGGCCGCAGGTGGATAACACGGGTGGGGAGCCGGCCTGGGACTCCCCGCTGCGGCG
  CGTCTTGGCCGAGCTGAACCGCATCCCCAGCAGCCGGCGGCGAGCGGCCCGCCTCTTTGAGTGGCTCATC
  GCGCCCATGCCGCCAGATCACTTTTACCGGCGCCTATGGGAGCGCGAGGCGGTGCTGGTGCGGCGGCAGG
  ACCACACCTACTACCAGGGACTTTTCTCTACCGCTGACCTGGATTCGATGCTGCGCAACGAGGAGGTGCA
  GTTCGGCCAGCATTTGGACGCCGCTCGCTACATCAACGGACGACGCGAGACCCTGAACCCACCCGGCCGC
  GCGCTGCCCGCCGCCGCGTGGTCCCTGTACCAGGCCGGCTGCTCCCTGCGTCTCCTCTGTCCGCAGGCTT
  TCTCTACTACTGTGTGGCAGTTTTTGGCTGTGCTTCAAGAGCAGTTTGGAAGCATGGCAGGCTCCAACGT
  TTACCTCACGCCCCCTAACTCGCAGGGCTTTGCCCCCCACTACGACGACATCGAGGCCTTCGTGCTGCAG
  CTGGAAGGTAGGAAACTCTGGCGTGTATACCGACCCCGAGTCCCAACCGAGGAACTGGCTCTGACATCCA
  GCCCCAACTTCAGTCAGGACGACCTCGGTGAGCCGGTGCTGCAGACCGTGCTGGAACCTGGAGATTTGCT
  GTATTTTCCTCGGGGCTTCATTCACCAAGCTGAATGCCAGGATGGAGTCCACTCTCTGCACCTCACCTTG
  TCCACGTACCAGCGCAATACCTGGGGTGACTTCTTAGAGGCCATACTGCCTCTGGCAGTGCAGGCTGCAA
  TGGAAGAAAATGTGGAGTTTCGGAGGGGTCTGCCCCGAGACTTCATGGATTACATGGGGGCCCAGCATTC
  AGATTCTAAGGATCCGCGAAGAACCGCTTTCATGGAGAAGGTGCGGGTCTTGGTTGCCCGCCTGGGACAC
  TTTGCTCCTGTTGATGCTGTGGCCGACCAGCGAGCCAAAGACTTCATTCACGATTCTCTGCCCCCTGTTT
  TGACTGATAGGGAGAGGGCACTAAGTGTTTACGGGCTTCCAATTCGCTGGGAGGCTGGAGAACCTGTAAA
  CGTGGGGGCCCAGTTGACAACAGAAACAGAAGTCCATATGCTTCAGGATGGGATAGCTCGGCTGGTGGGT
  GAGGGGGGCCATTTGTTTCTCTATTACACAGTGGAAAACTCCCGTGTGTATCATCTGGAAGAACCCAAGT
  GCTTGGAAATATACCCCCAGCAAGCTGATGCCATGGAACTGTTGCTTGGTTCTTATCCAGAGTTTGTGAG
  AGTGGGGGACCTGCCCTGTGACAGTGTGGAGGACCAGCTGTCCTTGGCAACCACGTTGTATGATAAGGGG
  CTGCTGCTCACTAAGATGCCTCTAGCCCTAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203678 representing NM_024644
  Red=Cloning site Green=Tags(s)
  
  MDGLQASAGPLRRGRPKRRRKPQPHSGSVLALPLRSRKIRKQLRSVVSRMAALRTQTLPSENSEESRVES
  TADDLGDALPGGAAVAAVPDAARREPYGHLGPAELLEASPAARSLQTPSARLVPASAPPARLVEVPAAPV
  RVVETSALLCTAQHLAAVQSSGAPATASGPQVDNTGGEPAWDSPLRRVLAELNRIPSSRRRAARLFEWLI
  APMPPDHFYRRLWEREAVLVRRQDHTYYQGLFSTADLDSMLRNEEVQFGQHLDAARYINGRRETLNPPGR
  ALPAAAWSLYQAGCSLRLLCPQAFSTTVWQFLAVLQEQFGSMAGSNVYLTPPNSQGFAPHYDDIEAFVLQ
  LEGRKLWRVYRPRVPTEELALTSSPNFSQDDLGEPVLQTVLEPGDLLYFPRGFIHQAECQDGVHSLHLTL
  STYQRNTWGDFLEAILPLAVQAAMEENVEFRRGLPRDFMDYMGAQHSDSKDPRRTAFMEKVRVLVARLGH
  FAPVDAVADQRAKDFIHDSLPPVLTDRERALSVYGLPIRWEAGEPVNVGAQLTTETEVHMLQDGIARLVG
  EGGHLFLYYTVENSRVYHLEEPKCLEIYPQQADAMELLLGSYPEFVRVGDLPCDSVEDQLSLATTLYDKG
  LLLTKMPLALN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024644
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024644.5
• RefSeq Size: 2463 bp
• RefSeq ORF: 1926 bp
• Locus ID: 79697
• UniProt ID: Q9H6W3
• Cytogenetics: 14q24.3
• Protein Families: Druggable Genome
• MW: 71.1 kDa
• Gene Summary: Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.[UniProtKB/Swiss-Prot Function]