SLC35F5 (NM_025181) Human Tagged ORF Clone

CAT#: RC203677

SLC35F5 (Myc-DDK-tagged)-Human solute carrier family 35, member F5 (SLC35F5)

Product Images

Circular map for RC203677

• 

  Western blot validation of overexpression lysate (Cat# LY403057) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203677 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC35F5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203677 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCGCCACGACGCCATCGCGGGGCAGGAAGGCCAGGGGTGCTGAGTTCTTCACCTCCTTTTAGAC
  TGAGATCTGCCAAGTTTTCCGGCATTGCTCTTGAGGATCTCAGAAGGGCTCTTAAGACAAGACTGCAAAT
  GGTGTGTGTATTTGTCATGAACCGAATGAATTCCCAGAACAGTGGTTTCACTCAGCGCAGGCGAATGGCT
  CTTGGGATTGTTATTCTTCTGCTTGTTGATGTGATATGGGTTGCTTCCTCTGAACTTACTTCGTATGTTT
  TTACCCAGTACAACAAACCATTCTTCAGCACCTTTGCAAAAACATCTATGTTTGTTTTGTACCTTTTGGG
  CTTTATTATTTGGAAGCCATGGAGACAACAGTGTACAAGAGGACTTCGCGGAAAGCATGCTGCTTTTTTT
  GCAGATGCTGAAGGTTACTTTGCTGCTTGCACAACAGATACAACTATGAATAGTTCTTTGAGTGAACCTC
  TGTATGTGCCTGTGAAATTCCATGATCTTCCAAGTGAAAAACCTGAGAGCACAAACATTGATACTGAAAA
  AACCCCCAAAAAGTCTCGTGTGAGGTTCAGTAATATCATGGAGATTCGACAGCTTCCGTCAAGTCATGCA
  TTGGAAGCAAAGTTGTCTCGCATGTCATATCCTGTGAAAGAACAAGAATCCATACTGAAAACTGTGGGGA
  AACTTACTGCAACTCAAGTAGCGAAAATTAGCTTTTTTTTTTGCTTTGTGTGGTTTTTGGCAAATTTGTC
  ATATCAAGAAGCACTTTCAGACACACAAGTTGCTATAGTTAATATTTTATCTTCAACTTCCGGACTTTTT
  ACCTTAATCCTTGCTGCAGTATTTCCAAGTAACAGTGGAGATAGATTTACCCTTTCTAAACTATTAGCTG
  TAATTTTAAGCATTGGAGGCGTTGTACTGGTAAACCTGGCAGGGTCTGAAAAACCTGCTGGAAGAGACAC
  AGTAGGTTCCATTTGGTCTCTTGCTGGAGCCATGCTCTATGCTGTCTATATTGTTATGATTAAGAGAAAA
  GTAGATAGAGAAGACAAGTTGGATATTCCAATGTTCTTTGGTTTTGTAGGTTTGTTTAATCTGCTGCTCT
  TATGGCCAGGTTTCTTTTTACTTCATTATACTGGATTTGAGGACTTCGAGTTTCCCAATAAAGTAGTATT
  AATGTGCATTATCATTAATGGCCTTATTGGAACAGTACTCTCAGAGTTCCTGTGGTTGTGGGGCTGCTTT
  CTTACCTCATCATTGATAGGCACACTTGCACTAAGCCTTACAATACCTCTGTCCATAATAGCTGACATGT
  GTATGCAAAAGGTGCAGTTTTCTTGGTTATTTTTTGCAGGAGCTATCCCTGTATTTTTTTCATTTTTTAT
  TGTAACTCTCCTATGCCATTATAATAATTGGGATCCTGTGATGGTGGGAATCAGAAGAATATTTGCTTTT
  ATATGCAGAAAACATCGAATTCAGAGAGTTCCAGAAGACAGCGAACAGTGTGAGAGTCTCATTTCTATGC
  ACAGTGTTTCTCAGGAGGATGGAGCTAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203677 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVPPRRHRGAGRPGVLSSSPPFRLRSAKFSGIALEDLRRALKTRLQMVCVFVMNRMNSQNSGFTQRRRMA
  LGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQCTRGLRGKHAAFF
  ADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEKTPKKSRVRFSNIMEIRQLPSSHA
  LEAKLSRMSYPVKEQESILKTVGKLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLF
  TLILAAVFPSNSGDRFTLSKLLAVILSIGGVVLVNLAGSEKPAGRDTVGSIWSLAGAMLYAVYIVMIKRK
  VDREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFEDFEFPNKVVLMCIIINGLIGTVLSEFLWLWGCF
  LTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPVFFSFFIVTLLCHYNNWDPVMVGIRRIFAF
  ICRKHRIQRVPEDSEQCESLISMHSVSQEDGAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025181
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025181.5
• RefSeq Size: 4412 bp
• RefSeq ORF: 1572 bp
• Locus ID: 80255
• UniProt ID: Q8WV83
• Cytogenetics: 2q14.1
• Domains: DUF6
• Protein Families: Transmembrane
• MW: 58.9 kDa
• Gene Summary: Putative solute transporter.[UniProtKB/Swiss-Prot Function]