WSCD1 (NM_015253) Human Tagged ORF Clone

CAT#: RC203651

WSCD1 (Myc-DDK-tagged)-Human WSC domain containing 1 (WSCD1)

Product Images

Circular map for RC203651

• 

  Western blot validation of overexpression lysate (Cat# LY414680) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203651 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WSCD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203651 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAACCTTTCTTCCGACTCCAGAAGTTTCTCCGCCGAACACAGTTCCTGCTGTTCTTCCTCACGG
  CTGCCTACCTGATGACCGGCAGCCTGCTGCTGCTGCAGCGGGTCCGCGTGGCTCTCCCACAGGGCCCCCG
  GGCACCCGGCCCCCTGCAGACCTTGCCAGTGGCCGCCGTGGCGCTGGGCGTGGGCTTGCTGGACAGCAGA
  GCCCTGCACGACCCTCGAGTCAGCCCAGAGCTGCTGCTGGGTGTGGACATGCTGCAGAGCCCCCTGACCC
  GGCCCCGGCCCGGCCCCCGCTGGCTCCGGAGCCGCAACTCGGAGCTGCGTCAGTTGCGTCGCCGCTGGTT
  CCACCACTTCATGAGTGACTCCCAGGGACCGCCCGCCCTGGGCCCCGAGGCTGCCAGGCCCGCCATCCAC
  AGCCGAGGCACCTACATTGGATGCTTCAGTGACGATGGCCACGAGAGGACTCTGAAAGGAGCTGTGTTTT
  ATGACTTGAGAAAGATGACTGTCTCCCACTGCCAGGATGCGTGTGCTGAGCGGTCCTATGTCTACGCCGG
  CTTGGAGGCCGGGGCGGAGTGTTACTGCGGGAACCGGCTGCCAGCGGTGAGCGTGGGGCTGGAAGAGTGT
  AACTATGAGTGCAAAGGCGAGAAGGGCTCTGTGTGCGGGGCTGTGGACCGGCTCTCCGTGTACCGTGTGG
  ACGAGCTGCAGCCGGGCTCCAGGAAGCGGCGGACCGCCACCTACCGCGGATGCTTCCGACTGCCAGAGAA
  CATCACACATGCCTTCCCCAGCTCCCTGATACAGGCCAATGTGACCGTGGGGACTTGCTCGGGCTTTTGT
  TCCCAGAAAGAGTTCCCCTTGGCCATTCTCAGGGGCTGGGAATGCTACTGTGCTTACCCTACCCCCCGGT
  TCAACCTGCGGGATGCCATGGACAGCTCAGTATGTGGCCAGGACCCTGAGGCACAGAGGCTGGCAGAATA
  CTGTGAGGTCTACCAGACACCTGTGCAAGACACTCGTTGTACAGACAGGAGGTTCCTGCCTAACAAATCC
  AAAGTGTTTGTGGCTTTGTCAAGCTTCCCAGGAGCCGGGAACACGTGGGCACGGCACCTCATTGAGCATG
  CCACTGGCTTCTATACAGGGAGCTACTACTTTGATGGAACCCTCTACAACAAAGGGTTCAAGGGCGAAAA
  GGACCACTGGCGGAGCCGACGCACCATCTGTGTCAAAACCCACGAGAGTGGCAGGAGGGAGATTGAGATG
  TTTGATTCAGCCATCCTGCTAATCCGGAACCCATACAGGTCCCTGGTGGCAGAATTCAACAGAAAATGTG
  CCGGGCACCTGGGATATGCAGCTGACCGCAACTGGAAGAGCAAAGAGTGGCCGGACTTTGTCAACAGCTA
  CGCCTCGTGGTGGTCCTCGCACGTCCTGGACTGGCTCAAGTACGGGAAGCGGCTGCTGGTGGTGCACTAC
  GAGGAGCTGCGGCGCAGCCTGGTGCCCACGTTACGGGAGATGGTGGCCTTCCTCAACGTGTCTGTGAGCG
  AGGAGCGGCTGCTCTGCGTGGAGAACAACAAGGAGGGCAGCTTCCGGCGGCGCGGCCGGCGCTCCCACGA
  CCCTGAGCCCTTCACCCCGGAGATGAAAGACTTGATCAATGGCTACATCCGGACGGTGGACCAAGCCCTG
  CGTGACCACAACTGGACGGGGCTGCCCAGGGAGTATGTGCCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203651 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKPFFRLQKFLRRTQFLLFFLTAAYLMTGSLLLLQRVRVALPQGPRAPGPLQTLPVAAVALGVGLLDSR
  ALHDPRVSPELLLGVDMLQSPLTRPRPGPRWLRSRNSELRQLRRRWFHHFMSDSQGPPALGPEAARPAIH
  SRGTYIGCFSDDGHERTLKGAVFYDLRKMTVSHCQDACAERSYVYAGLEAGAECYCGNRLPAVSVGLEEC
  NYECKGEKGSVCGAVDRLSVYRVDELQPGSRKRRTATYRGCFRLPENITHAFPSSLIQANVTVGTCSGFC
  SQKEFPLAILRGWECYCAYPTPRFNLRDAMDSSVCGQDPEAQRLAEYCEVYQTPVQDTRCTDRRFLPNKS
  KVFVALSSFPGAGNTWARHLIEHATGFYTGSYYFDGTLYNKGFKGEKDHWRSRRTICVKTHESGRREIEM
  FDSAILLIRNPYRSLVAEFNRKCAGHLGYAADRNWKSKEWPDFVNSYASWWSSHVLDWLKYGKRLLVVHY
  EELRRSLVPTLREMVAFLNVSVSEERLLCVENNKEGSFRRRGRRSHDPEPFTPEMKDLINGYIRTVDQAL
  RDHNWTGLPREYVPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015253
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015253.1, NP_056068.1
• RefSeq Size: 5827 bp
• RefSeq ORF: 1728 bp
• Locus ID: 23302
• UniProt ID: Q658N2
• Cytogenetics: 17p13.2
• Protein Families: Transmembrane
• MW: 65.7 kDa