RRP9 (NM_004704) Human Tagged ORF Clone

CAT#: RC203635

RRP9 (Myc-DDK-tagged)-Human ribosomal RNA processing 9, small subunit (SSU) processome component, homolog (yeast) (RRP9)

Product Images

Circular map for RC203635

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  Western blot validation of overexpression lysate (Cat# LY417831) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203635 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RRP9 protein (Cat# TP303635). The protein was produced from HEK293T cells transfected with RRP9 cDNA clone (Cat# RC203635) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RRP9
• Synonyms: RNU3IP2; U3-55K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203635 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCAACAGCGGCTGCTCGTAAGCGGGGAAAGCCGGCCTCTGGGGCCGGGGCTGGCGCGGGGGCCG
  GCAAGCGGCGGCGAAAGGCCGACTCTGCGGGGGACAGGGGCAAATCCAAGGGTGGCGGCAAGATGAATGA
  GGAGATCTCCAGCGACTCTGAGAGCGAGAGCCTAGCTCCAAGGAAGCCTGAGGAGGAGGAGGAGGAGGAG
  CTGGAGGAAACTGCACAGGAAAAGAAGCTGCGCTTGGCCAAGCTCTACCTAGAGCAGCTCCGTCAGCAAG
  AGGAGGAGAAGGCTGAGGCCCGTGCATTTGAGGAGGACCAGGTGGCGGGGCGCCTGAAGGAGGATGTGCT
  TGAGCAGAGGGGCAGGCTGCAGAAGTTGGTGGCAAAAGAGATCCAGGCCCCAGCCTCAGCTGACATTCGC
  GTTTTACGGGGGCACCAGCTCTCTATCACATGTTTGGTCGTCACCCCCGATGACTCAGCCATCTTCTCTG
  CTGCCAAAGACTGCAGCATCATTAAGTGGAGCGTGGAGAGTGGACGGAAGCTGCATGTGATTCCTCGAGC
  CAAGAAGGGTGCCGAGGGAAAGCCCCCTGGCCACAGCAGCCACGTCCTCTGCATGGCCATCTCCTCCGAC
  GGCAAGTACCTTGCCTCTGGTGACCGCAGCAAGCTCATTCTCATTTGGGAGGCCCAGAGCTGCCAGCACT
  TGTACACCTTCACAGGACACCGGGATGCAGTGTCGGGTCTGGCATTCCGCAGAGGCACCCACCAGCTCTA
  CAGCACATCCCACGATCGCTCCGTGAAGGTGTGGAATGTGGCAGAGAACTCCTACGTGGAGACGCTCTTC
  GGACACCAGGACGCTGTGGCTGCACTGGATGCCTTGAGCCGGGAGTGCTGTGTGACGGCTGGGGGCCGGG
  ATGGGACTGTACGTGTGTGGAAGATCCCCGAGGAGTCCCAGCTTGTCTTCTATGGCCACCAGGGCTCCAT
  CGACTGCATCCACCTAATCAATGAGGAGCACATGGTGTCCGGCGCGGACGATGGCTCTGTGGCCTTGTGG
  GGTCTCTCCAAGAAGCGACCACTTGCCCTGCAGCGTGAAGCTCACGGGCTGCGGGGAGAGCCAGGCCTGG
  AGCAGCCCTTCTGGATATCGTCGGTGGCAGCCCTCCTCAACACAGACCTTGTGGCCACAGGCTCCCACAG
  CTCCTGTGTGCGGCTTTGGCAGTGTGGGGAAGGCTTCCGGCAGCTTGACCTTCTCTGTGACATCCCCCTG
  GTGGGTTTTATCAACAGCCTCAAGTTCTCCAGCTCTGGGGACTTCCTGGTGGCTGGGGTAGGGCAGGAGC
  ACAGGCTTGGCCGATGGTGGAGAATCAAAGAGGCTCGGAATTCTGTCTGCATCATCCCACTCCGCAGGGT
  CCCTGTACCCCCAGCTGCTGGTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203635 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSATAAARKRGKPASGAGAGAGAGKRRRKADSAGDRGKSKGGGKMNEEISSDSESESLAPRKPEEEEEEE
  LEETAQEKKLRLAKLYLEQLRQQEEEKAEARAFEEDQVAGRLKEDVLEQRGRLQKLVAKEIQAPASADIR
  VLRGHQLSITCLVVTPDDSAIFSAAKDCSIIKWSVESGRKLHVIPRAKKGAEGKPPGHSSHVLCMAISSD
  GKYLASGDRSKLILIWEAQSCQHLYTFTGHRDAVSGLAFRRGTHQLYSTSHDRSVKVWNVAENSYVETLF
  GHQDAVAALDALSRECCVTAGGRDGTVRVWKIPEESQLVFYGHQGSIDCIHLINEEHMVSGADDGSVALW
  GLSKKRPLALQREAHGLRGEPGLEQPFWISSVAALLNTDLVATGSHSSCVRLWQCGEGFRQLDLLCDIPL
  VGFINSLKFSSSGDFLVAGVGQEHRLGRWWRIKEARNSVCIIPLRRVPVPPAAGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004704
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004704.5
• RefSeq Size: 1590 bp
• RefSeq ORF: 1428 bp
• Locus ID: 9136
• UniProt ID: O43818
• Cytogenetics: 3p21.2
• Domains: WD40
• MW: 51.8 kDa
• Gene Summary: This gene encodes a member of the WD-repeat protein family. The encoded protein is a component of the nucleolar small nuclear ribonucleoprotein particle (snoRNP) and is essential for 18s rRNA processing during ribosome synthesis. It contains seven WD domains required for nucleolar localization and specific interaction with the U3 small nucleolar RNA (U3 snoRNA). [provided by RefSeq, Oct 2012]