CCDC28B (NM_024296) Human Tagged ORF Clone
CAT#: RC203631
CCDC28B (Myc-DDK-tagged)-Human coiled-coil domain containing 28B (CCDC28B)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_024296" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CCDC28B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203631 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGACAAAAAGAAGAAACGGAGTCCCAAGCCCTGCCTGGCCCAGCCAGCCCAGGCCCCAGGCACAC TACGGAGGGTCCCTGTGCCTACCAGCCACAGCGGCTCCTTGGCCCTAGGACTTCCTCATCTGCCATCCCC CAAGCAGCGGGCCAAGTTCAAGAGAGTAGGCAAAGAGAAGTGCCGCCCAGTCCTGGCTGGAGGTGGAAGC GGCTCTGCAGGCACGCCCCTGCAGCACTCCTTCCTGACCGAGGTGACTGATGTCTATGAGATGGAGGGGG GACTCCTGAACCTGCTCAATGATTTCCACTCTGGCCGGCTGCAGGCCTTCGGGAAGGAATGCTCCTTTGA GCAGCTGGAGCACGTTCGGGAGATGCAGGAGAAGCTAGCCCGGCTGCACTTCAGCCTGGATGTGTGTGGG GAGGAGGAGGACGATGAAGAGGAAGAGGATGGGGTCACTGAGGGGCTGCCAGAGGAGCAGAAGAAGACAA TGGCTGACCGTAACCTGGACCAGCTGCTTAGCAATCTGGAAGACCTCAGTAATTCTATCCAGAAGCTGCA CCTGGCCGAGAACGCCGAGCCTGAGGAGCAGTCCGCTGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203631 protein sequence
Red=Cloning site Green=Tags(s) MDDKKKKRSPKPCLAQPAQAPGTLRRVPVPTSHSGSLALGLPHLPSPKQRAKFKRVGKEKCRPVLAGGGS GSAGTPLQHSFLTEVTDVYEMEGGLLNLLNDFHSGRLQAFGKECSFEQLEHVREMQEKLARLHFSLDVCG EEEDDEEEEDGVTEGLPEEQKKTMADRNLDQLLSNLEDLSNSIQKLHLAENAEPEEQSAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_024296 |
ORF Size | 600 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024296.5 |
RefSeq Size | 1110 bp |
RefSeq ORF | 603 bp |
Locus ID | 79140 |
UniProt ID | Q9BUN5 |
Cytogenetics | 1p35.2 |
MW | 22 kDa |
Gene Summary | The product of this gene localizes to centrosomes and basal bodies. The protein colocalizes with several proteins associated with Bardet-Biedl syndrome (BBS) and participates in the regulation of cilia development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203631L3 | Lenti ORF clone of Human coiled-coil domain containing 28B (CCDC28B), Myc-DDK-tagged |
USD 600.00 |
|
RC203631L4 | Lenti ORF clone of Human coiled-coil domain containing 28B (CCDC28B), mGFP tagged |
USD 600.00 |
|
RG203631 | CCDC28B (tGFP-tagged) - Human coiled-coil domain containing 28B (CCDC28B) |
USD 500.00 |
|
SC111413 | CCDC28B (untagged)-Human coiled-coil domain containing 28B (CCDC28B) |
USD 300.00 |
{0} Product Review(s)
Be the first one to submit a review