CDCA5 (NM_080668) Human Tagged ORF Clone

CAT#: RC203624

Reviews ()
Write a review

CDCA5 (Myc-DDK-tagged)-Human cell division cycle associated 5 (CDCA5)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CDCA5
Synonyms SORORIN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203624 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGGAGGCGAACGCGGTCCGGAGGAGCCGCTCAGCGCTCCGGGCCAAGGGCCCCATCTCCTACTA
AGCCTCTGCGGAGGTCCCAGCGGAAATCAGGCTCTGAACTCCCGAGCATCCTCCCTGAAATCTGGCCGAA
GACACCCAGTGCGGCTGCAGTCAGAAAGCCCATCGTCTTAAAGAGGATCGTGGCCCATGCTGTAGAGGTC
CCAGCTGTCCAATCACCTCGCAGGAGCCCTAGGATTTCCTTTTTCTTGGAGAAAGAAAACGAGCCCCCTG
GCAGGGAGCTTACTAAGGAGGACCTTTTCAAGACACACAGCGTCCCTGCCACCCCCACCAGCACTCCTGT
GCCGAACCCTGAGGCCGAGTCCAGCTCCAAGGAAGGAGAGCTGGACGCCAGAGACTTGGAAATGTCTAAG
AAAGTCAGGCGTTCCTACAGCCGGCTGGAGACCCTGGGCTCTGCCTCTACCTCCACCCCAGGCCGCCGGT
CCTGCTTTGGCTTCGAGGGGCTGCTGGGGGCAGAAGACTTGTCCGGAGTCTCGCCAGTGGTGTGCTCCAA
ACTCACCGAGGTCCCCAGGGTTTGTGCAAAGCCCTGGGCCCCAGACATGACTCTCCCTGGAATCTCCCCA
CCACCCGAGAAACAGAAACGTAAGAAGAAGAAAATGCCAGAGATCTTGAAAACGGAGCTGGATGAGTGGG
CTGCGGCCATGAATGCCGAGTTTGAAGCTGCTGAGCAGTTTGATCTCCTGGTTGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203624 protein sequence
Red=Cloning site Green=Tags(s)

MSGRRTRSGGAAQRSGPRAPSPTKPLRRSQRKSGSELPSILPEIWPKTPSAAAVRKPIVLKRIVAHAVEV
PAVQSPRRSPRISFFLEKENEPPGRELTKEDLFKTHSVPATPTSTPVPNPEAESSSKEGELDARDLEMSK
KVRRSYSRLETLGSASTSTPGRRSCFGFEGLLGAEDLSGVSPVVCSKLTEVPRVCAKPWAPDMTLPGISP
PPEKQKRKKKKMPEILKTELDEWAAAMNAEFEAAEQFDLLVE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080668
ORF Size 756 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080668.4
RefSeq Size 2599 bp
RefSeq ORF 759 bp
Locus ID 113130
UniProt ID Q96FF9
Cytogenetics 11q13.1
Protein Families Druggable Genome
MW 27.6 kDa
Gene Summary Regulator of sister chromatid cohesion in mitosis stabilizing cohesin complex association with chromatin. May antagonize the action of WAPL which stimulates cohesin dissociation from chromatin. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Required for efficient DNA double-stranded break repair.[UniProtKB/Swiss-Prot Function]
Other products for "CDCA5"
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.