UAP1 (NM_003115) Human Tagged ORF Clone

CAT#: RC203494

UAP1 (Myc-DDK-tagged)-Human UDP-N-acteylglucosamine pyrophosphorylase 1 (UAP1)

Product Images

Circular map for RC203494

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  Western blot validation of overexpression lysate (Cat# LY418892) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203494 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UAP1 protein (Cat# TP303494). The protein was produced from HEK293T cells transfected with UAP1 cDNA clone (Cat# RC203494) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UAP1
• Synonyms: AGX; AGX1; AGX2; SPAG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203494 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATTAATGACCTCAAACTCACGTTGTCCAAAGCTGGGCAAGAGCACCTACTACGTTTCTGGAATG
  AGCTTGAAGAAGCCCAACAGGTAGAACTTTATGCAGAGCTCCAGGCCATGAACTTTGAGGAGCTGAACTT
  CTTTTTCCAAAAGGCCATTGAAGGTTTTAACCAGTCTTCTTACCAAAAGAATGTGGATGCACGAATGGAA
  CCTGTGCCTCGAGAGGTATTAGGCAGTGCTACAAGGGATCAAGATCAGCTCCAGGCCTGGGAAAGTGAAG
  GACTTTTCCAGATTTCTCAGAATAAAGTAGCAGTTCTTCTTCTAGCTGGTGGGCAGGGGACAAGACTCGG
  CGTTGCATATCCTAAGGGGATGTATGATGTTGGTTTGCCATCCCGTAAGACACTTTTTCAGATTCAAGCA
  GAGCGTATCCTGAAGCTACAGCAGGTTGCTGAAAAATATTATGGCAACAAATGCATTATTCCATGGTATA
  TAATGACCAGTGGCAGAACAATGGAATCTACAAAGGAGTTCTTCACCAAGCACAAGTACTTTGGTTTAAA
  AAAAGAGAATGTAATCTTTTTTCAGCAAGGAATGCTCCCCGCCATGAGTTTTGATGGGAAAATTATTTTG
  GAAGAGAAGAACAAAGTTTCTATGGCTCCAGATGGGAATGGTGGTCTTTATCGGGCACTTGCAGCCCAGA
  ATATTGTGGAGGATATGGAGCAAAGAGGCATTTGGAGCATTCATGTCTATTGTGTTGACAACATATTAGT
  AAAAGTGGCAGACCCACGGTTCATTGGATTTTGCATTCAGAAAGGAGCAGACTGTGGAGCAAAGGTGGTA
  GAGAAAACGAACCCTACAGAACCAGTTGGAGTGGTTTGCCGAGTGGATGGAGTTTACCAGGTGGTAGAAT
  ATAGTGAGATTTCCCTGGCAACAGCTCAAAAACGAAGCTCAGACGGACGACTGCTGTTCAATGCGGGGAA
  CATTGCCAACCATTTCTTCACTGTACCATTTCTGAGAGATGTTGTCAATGTTTATGAACCTCAGTTGCAG
  CACCATGTGGCTCAAAAGAAGATTCCTTATGTGGATACCCAAGGACAGTTAATTAAGCCAGACAAACCCA
  ATGGAATAAAGATGGAAAAATTTGTCTTTGACATCTTCCAGTTTGCAAAGAAGTTTGTGGTATATGAAGT
  ATTGCGAGAAGATGAGTTTTCCCCACTAAAGAATGCTGATAGTCAGAATGGGAAAGACAACCCTACTACT
  GCAAGGCATGCTTTGATGTCCCTTCATCATTGCTGGGTCCTCAATGCAGGGGGCCATTTCATAGATGAAA
  ATGGCTCTCGCCTTCCAGCAATTCCCCGCTTGAAGGATGCCAATGATGTACCAATCCAATGTGAAATCTC
  TCCTCTTATCTCCTATGCTGGAGAAGGATTAGAAAGTTATGTGGCAGATAAAGAATTCCATGCACCTCTA
  ATCATCGATGAGAATGGAGTTCATGAGCTGGTGAAAAATGGTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203494 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSYQKNVDARME
  PVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQA
  ERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIIL
  EEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVV
  EKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQ
  HHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTT
  ARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPL
  IIDENGVHELVKNGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003115
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003115.3, NP_003106.2
• RefSeq Size: 2344 bp
• RefSeq ORF: 1518 bp
• Locus ID: 6675
• UniProt ID: Q16222
• Cytogenetics: 1q23.3
• Domains: UDPGP
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Metabolic pathways
• MW: 57.1 kDa
• Gene Summary: Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.[UniProtKB/Swiss-Prot Function]